Diversity and expression of P450 genes from Dendroctonus valens LeConte (Curculionidae: Scolytinae) in response to different kairomones

López, María Fernanda; Cano-Ramírez, Claudia; Cesar-Ayala, Ana K.; Ruíz, Enrico A.; Zúñiga, Gerardo

doi:10.1016/j.ibmb.2013.02.004

Cited by 50 publications

(54 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, changes in transcripts annotated as cytochrome P450s in the closely related Dendroctonus spp. midguts, fatbodies, and antennae were shown to increase in response to common conifer host monoterpenes [31]–[32]. Our data showed a large significant female-specific increase in CYP6DH1.…”

Section: Discussionmentioning

confidence: 49%

Disentangling Detoxification: Gene Expression Analysis of Feeding Mountain Pine Beetle Illuminates Molecular-Level Host Chemical Defense Detoxification Mechanisms

et al. 2013

View full text Add to dashboard Cite

The mountain pine beetle, Dendroctonus ponderosae, is a native species of bark beetle (Coleoptera: Curculionidae) that caused unprecedented damage to the pine forests of British Columbia and other parts of western North America and is currently expanding its range into the boreal forests of central and eastern Canada and the USA. We conducted a large-scale gene expression analysis (RNA-seq) of mountain pine beetle male and female adults either starved or fed in male-female pairs for 24 hours on lodgepole pine host tree tissues. Our aim was to uncover transcripts involved in coniferophagous mountain pine beetle detoxification systems during early host colonization. Transcripts of members from several gene families significantly increased in insects fed on host tissue including: cytochromes P450, glucosyl transferases and glutathione S-transferases, esterases, and one ABC transporter. Other significantly increasing transcripts with potential roles in detoxification of host defenses included alcohol dehydrogenases and a group of unexpected transcripts whose products may play an, as yet, undiscovered role in host colonization by mountain pine beetle.

show abstract

Section: Discussionmentioning

confidence: 49%

Disentangling Detoxification: Gene Expression Analysis of Feeding Mountain Pine Beetle Illuminates Molecular-Level Host Chemical Defense Detoxification Mechanisms

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In addition, the expression level of antennal P450s was also influenced by the host volatile in the red turpentine beetle, De. valens (López et al ., ), and the pine beetle, De. rhizophagus (Cano‐Ramírez et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…High expression of NADPH‐P450 oxidoreductases and cytochrome P450 in the antennae reflect their putative function in insect olfaction (Hovemann et al ., ). Four P450s and a P450 reductase were reported in the antennae of the cabbage armyworm, Mamestra brassicae (Maïbèche‐Coisne et al ., ), three P450s in the pale‐brown chafer, Phyllopertha diversa (Maïbèche‐Coisne et al ., ), 10 P450s (five CYP4, four CYP6 and one CYP9) in the pine beetle, Dendroctonus rhizophagus (Cano‐Ramírez et al ., ) and 11 P450s (five CYP4, four CYP6 and two CYP9) in the red turpentine beetle, Dendroctonus valens (López et al ., ). Moreover, in P. diversa , it was reported that PdivCYP4AW1, which has antenna‐specific expression, has a pheromone‐degrading function and is used to quickly inactivate sex pheromones (Maïbèche‐Coisne et al ., ).…”

Section: Introductionmentioning

confidence: 99%

A cytochrome P450 gene plays a role in the recognition of sex pheromones in the tobacco cutworm, Spodoptera litura

Feng

Zheng

et al. 2017

Insect Molecular Biology

View full text Add to dashboard Cite

Cytochrome P450 (P450 or CYP) genes are involved in fundamental physiological functions, and might be also associated with the olfactory recognition of sex pheromones in beetles and moths. A P450 gene, Spodoptera litura CYP4L4 (SlituCYP4L4), was cloned for the first time from the antennae of S. litura. SlituCYP4L4 was almost exclusively expressed in the adult stage and predominantly expressed in the adult antennae. In situ hybridization showed that SlituCYP4L4 localized mainly at the base of the long sensilla trichoidea, which responds to sex pheromone components. Pretreatment with an S. litura sex pheromone significantly reduced the expression levels of SlituCYP4L4, consistent with other genes involved in sex pheromone recognition. The expression level of SlituCYP4L4 was different in moths collected with different ratios of sex pheromone lures and collected in different geographical locations. After gene knockdown of SlituCYP4L4 in the antennae, the electroantennogram (EAG) responses of male and female moths to (9Z,11E)-tetradecadienyl acetate or (9Z,12E)-tetradecadienyl acetate were significantly decreased. In contrast, EAG responses to plant volatiles and sex pheromones of other moth species were not significantly influenced in these moths. SlituCYP4L4 was also expressed in the gustatory tissues and sensilla, which suggests that SlituCYP4L4 may have other functions in the chemosensory system. Our results have shown for the first time the function of a CYP gene with appendage-specific expression in insect sex pheromone recognition, especially in adult moths.

show abstract

“…Interactions between conifer resistance and bark beetle colonization efforts were also associated with allelochemicals (Raffa & Berryman, 1983). In the field, D. valens was observed to attack large pine trees (Liu et al, 2011) as well as fresh stumps (Owen et al, 2010;L opez et al, 2013). Do trees containing more monoterpenes better resist attacking beetles?…”

Section: Discussionmentioning

confidence: 99%

The effects of α‐pinene on the feeding performance and pheromone production of Dendroctonus valens

Liu

Sun

2014

Entomologia Exp Applicata

View full text Add to dashboard Cite

Herbivorous insects exploit multiple plant cues to detect and orient toward suitable hosts and, accordingly, hosts have evolved complex constitutive and inducible defenses in response. In China, the red turpentine beetle, Dendroctonus valens LeConte (Coleoptera: Curculionidae: Scolytinae), an invasive bark beetle from North America, attacks mainly Pinus tabuliformis Carri ere (Pinaceae), which contains many monoterpenes. In this study, we explored how the monoterpene a-pinene affects the feeding performance and pheromone production of D. valens. First, the composition and quantities of monoterpenes of both P. tabuliformis healthy trees and fresh stumps were determined and the infestation of D. valens in healthy trees and fresh stumps was investigated, linking the amount of monoterpenes and D. valens infestation. Gas chromatography-mass spectrometry (GC-MS) analysis showed that P. tabuliformis mainly contained a-pinene, with concentrations of 0.1 and 0.5 mg g À1 in healthy pine phloem and stump phloem, respectively. Second, the monoterpene's influence on feeding performance was tested using phloem media with a-pinene concentrations ranging from 0 to 30 mg g À1 . The results showed that the percentages of beetles boring and the gallery lengths of both adult females and larvae were negatively correlated with the a-pinene concentration although body weight changes did not correlate with a-pinene concentration. Finally, pheromone analysis showed that the production of all pheromones increased with increasing a-pinene concentrations. This study showed the dual effects of a-pinene on D. valens: a-pinene inhibited the bark beetle's feeding activities and in turn the bark beetle made use of it to produce pheromones. Our study indicated the importance of promptly removing fresh stumps in the field for the management of the bark beetle.

show abstract

Diversity and expression of P450 genes from Dendroctonus valens LeConte (Curculionidae: Scolytinae) in response to different kairomones

Cited by 50 publications

References 64 publications

Disentangling Detoxification: Gene Expression Analysis of Feeding Mountain Pine Beetle Illuminates Molecular-Level Host Chemical Defense Detoxification Mechanisms

Disentangling Detoxification: Gene Expression Analysis of Feeding Mountain Pine Beetle Illuminates Molecular-Level Host Chemical Defense Detoxification Mechanisms

A cytochrome P450 gene plays a role in the recognition of sex pheromones in the tobacco cutworm, Spodoptera litura

The effects of α‐pinene on the feeding performance and pheromone production of Dendroctonus valens

Contact Info

Product

Resources

About