Diversity and phylogeny of percolomonads based on newly discovered species from hypersaline and marine waters

Carduck, Sarah; Nitsche, Frank; Rybarski, Alexandra; Hohlfeld, Manon; Arndt, Hartmut

doi:10.1016/j.ejop.2021.125808

Cited by 7 publications

(17 citation statements)

References 26 publications

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“…Metabarcodes (unique V9 sequences) of the Tara Ocean project were used for analyzing distribution patterns of our strains in marine surface waters. We taxonomically assigned the metabarcodes of both datasets with VSEARCH's (Rognes et al, 2016) global pairwise alignment option, using a reference database consisting of the V9‐sequences of the seven newly isolated Percolomonas ‐like strains, seven additional strains from the HFCC culture collection (Carduck et al, 2021) and all available V9‐sequences of Percolomonadida from GenBank (Table 1). We analyzed the read abundance of metabarcodes assigned to the Percolomonas ‐like sequences with a sequence similarity of 100%.…”

Section: Methodsmentioning

confidence: 99%

“…Lula levis sp. nov. survives no changes of salinity in culture (Figure 5), whereas L. jakobsenorum is able to grow in salinities from 23 to 50 PSU (Carduck et al, 2021). The three short flagella are shorter than the protoplast, which was also observed for L. jakobsenorum .…”

Section: Taxonomic Summarymentioning

confidence: 99%

“…Cultures of B. pacifica sp. nov. are able to survive salinities of up to 50 PSU (Figure 5), while the other Barbelia species are only able to grow in salinities up to 36 PSU (Carduck et al, 2021). While all three Barbelia species were isolated form marine waters, B. abyssalis and B. atlantica derived from the North Atlantic Ocean, whereas B. pacifica sp.…”

Section: Taxonomic Summarymentioning

confidence: 99%

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Biogeography, autecology, and phylogeny of Percolomonads based on newly described species

Hohlfeld

Meyer

Schoenle

et al. 2022

J Eukaryotic Microbiology

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Percolomonads (Heterolobosea) are aquatic heterotrophic flagellates frequently found in saline waters up to hypersaline environments. We isolated and cultivated seven strains of percolomonad flagellates from marine waters and sediments as well as from a hypersaline inland lake in the Atacama Desert. Morphological characterizations, comprising light and scanning electron microscopy, revealed only slight differences between the strains mainly limited to the cell shape, length of flagella, and length of the ventral feeding groove. Phylogenetic analyses of the 18S and 28S rDNA genes showed the formation of three fully supported clades within the Percolomonadida: the Percolomonadidae, the Barbeliidae fam. nov. and the Lulaidae fam. nov. We describe two new families (Barbeliidae fam. nov., Lulaidae fam. nov.), a new genus (Nonamonas gen. nov.), and five new species (Percolomonas adaptabilis sp. nov., Lula levis sp. nov., Barbelia pacifica sp. nov., Nonamonas montiensis gen. et sp. nov., Nonamonas santamariensis gen. et sp. nov.). Salinity experiments showed that P. adaptabilis sp. nov. from the Atlantic was better adapted to high salinities than all other investigated strains. Moreover, comparisons of our cultivation‐based approach with environmental sequencing studies showed that P. adaptabilis sp. nov. seems to be globally distributed in marine surface waters while other species seem to be more locally restricted.

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Section: Methodsmentioning

confidence: 99%

Section: Taxonomic Summarymentioning

confidence: 99%

Section: Taxonomic Summarymentioning

confidence: 99%

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Biogeography, autecology, and phylogeny of Percolomonads based on newly described species

Hohlfeld

Meyer

Schoenle

et al. 2022

J Eukaryotic Microbiology

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“…The 18S rRNA gene sequences from 68 representative heterolobosean species, including isolate ES010, plus sequences from 16 representative non-heterolobosean species selected as outgroups, were used for phylogenetic analysis (the seed alignment originated from Tikhonenkov et al, 2019;Carduck et al, 2021). The dataset was aligned using the MAFFT program v.7 (Katoh and Standley, 2013) and masked by eye, with 1,348 sites retained.…”

Section: Molecular Sequencing and Phylogenymentioning

confidence: 99%

“…Heterolobosea is regarded as a crucial group to better understand adaptation in halophilic/ halotolerant eukaryotes because it includes several contrasting clades of these organisms (Kirby et al, 2015;Harding and Simpson, 2018;Jhin and Park, 2019). Recently, several studies have demonstrated that many halophilic/halotolerant eukaryotes (e.g., Aurem, Euplaesiobystra, Percolomonas, Pharyngomonas, Pleurostomum, Selenaion, and Tulameoba) are novel genera belonging to Heterolobosea (Park et al, 2007(Park et al, , 2009(Park et al, , 2012Park and Simpson, 2011;Jhin and Park, 2019;Tikhonenkov et al, 2019;Aucher et al, 2020;Carduck et al, 2021). In addition to expanding the known diversity of halophilic eukaryotes, these studies suggest that a more complex food web than previously thought may function in hypersaline environments (Lee et al, 2022a).…”

Section: Introductionmentioning

confidence: 99%

Accumulation patterns of intracellular salts in a new halophilic amoeboflagellate, Euplaesiobystra salpumilio sp. nov., (Heterolobosea; Discoba) under hypersaline conditions

2022

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Halophilic microbial eukaryotes are present in many eukaryotic lineages and major groups; however, our knowledge of their diversity is still limited. Furthermore, almost nothing is known about the intracellular accumulation of salts in most halophilic eukaryotes. Here, we isolate a novel halophilic microbial eukaryote from hypersaline water of 134 practical salinity units (PSU) in a solar saltern. This species is an amoeboflagellate (capable of the amoeba-flagellate-cyst transformation) in the heterolobosean group and belongs to the genus Euplaesiobystra based on morphological data and 18S rDNA sequences. However, the isolate is distinct from any of the described Euplaesiobystra species. Especially, it is the smallest Euplaesiobystra to date, has a distinct cytostome, and grows optimally at 75–100 PSU. Furthermore, the phylogenetic tree of the 18S rDNA sequences demonstrates that the isolate forms a strongly supported group, sister to Euplaesiobystra hypersalinica. Thus, we propose that the isolate, Euplaesiobystra salpumilio, is a novel species. E. salpumilio displays a significantly increased influx of the intracellular Na+ and K+ at 50, 100, and 150 PSU, compared to freshwater species. However, the intracellular retention of the Na+ and K+ at 150 PSU does not significantly differ from 100 PSU, suggesting that E. salpumilio can extrude the Na+ and K+ from cells under high-salinity conditions. Interestingly, actively growing E. salpumilio at 100 and 150 PSU may require more intracellular accumulation of Na+ than the no-growth but-viable state at 50 PSU. It seems that our isolate displays two salt metabolisms depending on the tested salinities. E. salpumilio shows a salt-in strategy for Na+ at lower salinity of 100 PSU, while it displays a salt-out strategy for Na+ at higher salinity of 150 PSU. Our results suggest that the novel halophilic E. salpumilio fundamentally uses a salt-out strategy at higher salinities, and the accumulation patterns of intracellular salts in this species are different from those in other halophilic microbial eukaryotes.

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Cafeteria in extreme environments: Investigations on C. burkhardae and three new species from the Atacama Desert and the deep ocean

Schoenle

Hohlfeld

Rybarski

et al. 2022

European Journal of Protistology

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Diversity and phylogeny of percolomonads based on newly discovered species from hypersaline and marine waters

Cited by 7 publications

References 26 publications

Biogeography, autecology, and phylogeny of Percolomonads based on newly described species

Biogeography, autecology, and phylogeny of Percolomonads based on newly described species

Accumulation patterns of intracellular salts in a new halophilic amoeboflagellate, Euplaesiobystra salpumilio sp. nov., (Heterolobosea; Discoba) under hypersaline conditions

Cafeteria in extreme environments: Investigations on C. burkhardae and three new species from the Atacama Desert and the deep ocean

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