Diversity and potential application of endophytic bacteria in ginger

Abstract

ABSTRACT. Here, 248 endophytic bacterial strains were isolated to assess the distribution and population diversity of endophytic bacteria in ginger plants. A total of 10.4 x 10 4 to 20.2 x 10 4 CFU/g fresh weight endophytic bacteria of different growth stages were isolated. Maximum bacterium numbers were obtained at the seedling stage. A total of 107 functional strains were screened, including 17 antibacterial strains and 90 indole acetic acid-producing strains. Based on 16S rDNA sequence restriction fragment length polymorphism and 16S rDNA sequences, these 107 strains were mapped and grouped into 16 genera. Bacillus and Pseudomonas were the dominant genera; however, the bacteria belonged to a tremendous range of genera, with the highest species richness being observed at the seedling stage. Sixteen strains exhibited antimicrobial activity against Pythium myriotylum Drechsler, while 7 strains exhibited antimicrobial activity against Phyllosticta zingiberi Hori. Bacillus was the dominant antibacterial strain. Pseudomonas fluorescens, B. megaterium, and Enterobacter ludwigii produced remarkably high levels of IAA. Only a few endophytic bacterial Diversity and potential application of endophytic bacteria strains were inhibited in fresh ginger juice. Most of these strains were present during seedling stage, including Roseateles depolymerans, Chryseobacterium taiwanense, E. ludwigii, Agrobacterium larrymoorei, P. fluorescens, and Bacillus amyloliquefaciens. This study indicates that the community of endophytic bacteria in ginger changes with the synthesis of antibacterial substances.

High-throughput sequencing-based analysis of the composition and diversity of endophytic bacterial community in seeds of upland rice

“…Once they enter the root, endophytes can infect seeds have also changed. In the past reports, the researchers used the methods of conventional culture-dependent method (Liu et al 2009;Jiang et al 2013), 16S rDNA clone library technique ( Liu et al 2012;Zou et al 2012;Chen et al 2014;Haimin et al 2014) and deformable gradient gel electrophoresis (DGGE) to study endophytes (Hardoim et al 2012). In recent years, with the rise of high-throughput sequencing technology, it has been widely used in plant seed endophyte community structure and diversity analysis.…”

Genotyping and identification of broad spectrum antimicrobial volatiles in black pepper root endophytic biocontrol agent, Bacillus megaterium BP17

“…Aravind et al (2012) reported Bacillus megaterium based pre-planting bacterization technology for production of healthy black pepper rooted cuttings. Other reports of plant endophytic B. megaterium are from cotton, ginger and ginseng (McInroy and Kloepper, 1995;Vendan et al, 2010;Chen et al, 2014).…”

Molecular phylogeny and biotechnological potential of bacterial endophytes associated with Malpighia emarginata

“…Endophytic bacteria of the genus Pseudomonas have already been isolated from several plant species, including Eucalyptus sp, sugar cane, and ginger (Procópio et al, 2009;Magnani et al, 2013;Chen et al, 2014). Kumar et al (2014) isolated endophytic bacteria from Cassia tora roots and identified the species Pseudomonas putida among the isolates.…”