Diversity and structural differences of bacterial microbial communities in rhizocompartments of desert leguminous plants

Abstract

By assessing diversity variations of bacterial communities under different rhizocompartment types (i.e., roots, rhizosphere soil, root zone soil, and inter-shrub bulk soil), we explore the structural difference of bacterial communities in different root microenvironments under desert leguminous plant shrubs. Results will enable the influence of niche differentiation of plant roots and root soil on the structural stability of bacterial communities under three desert leguminous plant shrubs to be examined. High-throughput 16S rRNA genome sequencing was used to characterize diversity and structural differences of bacterial microbes in the rhizocompartments of three xeric leguminous plants. Results from this study confirm previous findings relating to niche differentiation in rhizocompartments under related shrubs, and they demonstrate that diversity and structural composition of bacterial communities have significant hierarchical differences across four rhizocompartment types under leguminous plant shrubs. Desert leguminous plants showed significant hierarchical filtration and enrichment of the specific bacterial microbiome across different rhizocompartments (P < 0.05). The dominant bacterial microbiome responsible for the differences in microbial community structure and composition across different niches of desert leguminous plants mainly consisted of Proteobacteria, Actinobacteria, and Bacteroidetes. All soil factors of rhizosphere and root zone soils, except for NO3—N and TP under C. microphylla and the two Hedysarum spp., recorded significant differences (P < 0.05). Moreover, soil physicochemical factors have a significant impact on driving the differentiation of bacterial communities under desert leguminous plant shrubs. By investigating the influence of niches on the structural difference of soil bacterial communities with the differentiation of rhizocompartments under desert leguminous plant shrubs, we provide data support for the identification of dominant bacteria and future preparation of inocula, and provide a foundation for further study of the host plants-microbial interactions.

The Macleaya cordata Symbiont: Revealing the Effects of Plant Niches and Alkaloids on the Bacterial Community

“…They migrated and colonized the area around the root of plants under the influence of the roots’ secretions ( Tahtamouni et al, 2015 ). Rhizospheric microbial community composition was significantly influenced by the physical and chemical parameters in soil ( Winston et al, 2017 ; Hartman et al, 2018 ; Ziyuan et al, 2020 ), which also affected the plant compartments in part ( Mighell et al, 2019 ). However, Two studies on Arabidopsis showed that the endophytic microbial community composition in the Arabidopsis plants growing on four different soils was similar ( Davide et al, 2012 ; Lundberg et al, 2012 ), indicating the existence of host mediated control mechanisms.…”

Microbial Assemblages Associated with the Soil-Root Continuum of an Endangered Plant, Helianthemum songaricum Schrenk

“…So there are a number of soil factors (e.g., nutrient availability, phosphorus, and pH) that affect the root-soil-associated microbiomes. The differences in these key soil factors reflected the differences in the responses of the microbiome communities of shrub plants under arid conditions ( 64 ).…”

Strong homogenization effects of shrubs on nematode communities across large spatial scales on the Qinghai-Tibet Plateau

“…Moreover, the rate of shrub encroachment (2.1%) is high when compared with elsewhere (0.1-2.3%) during the same period, and at least 39% of grassland areas are encroached, which will further affect soil nematode communities on the Qinghai-Tibet Plateau (Brandtet al , 2013). However, the current research results of shrub effects on soil nematode communities are not consistent (Barber & Marquis, 2011;Zhang et al, 2020;Zhou et al, 2020), and there is also a lack of research about the large-scale processes that are involved in responses to amplifying precipitation and temperature. In order to take large-scale processes into account, we examined nematode communities, plant communities, and soil properties across a 1200 km x 1200 km area with widespread shrub on the Qinghai-Tibet Plateau.…”

“…In order to take large-scale processes into account, we examined nematode communities, plant communities, and soil properties across a 1200 km x 1200 km area with widespread shrub on the Qinghai-Tibet Plateau. We tested the following hypotheses: 1) shrubs decrease the nematode beta diversity and the nestedness component (Trentanoviet al , 2013;Dar & Reshi, 2014;Zhang et al , 2019); 2) leguminous shrub plays a stronger role in decreasing the nematode beta diversity compared with non-leguminous shrubs due to their nitrogen fixation capacity (Zhou et al , 2020); 3) shrub effects on nematode beta diversity increases with MAT and MAP, because shrubs are more stable in response to climate change than open space (Hao et al , 2021); 4) shrub affects beta diversity of nematode communities directly and indirectly through changes in beta diversity of plant communities and soil heterogeneity (Zhang et al , 2019).…”