Diversity in Indian Equine Rotaviruses: Identification of Genotype G10,P6[1] and G1 Strains and a New VP7 Genotype (G16) Strain in Specimens from Diarrheic Foals in India

Abstract

Rotaviruses causing severe diarrhea in foals in two organized farms in northern India, during the period from 2003 to 2005, were characterized by electropherotyping, serotyping, and sequence analysis of the genes encoding the outer capsid proteins. Of 137 specimens, 47 (34.31%) were positive for rotavirus and exhibited at least five different electropherotypes (E), E1 to E5. Strains belonging to different electropherotypes exhibited either a different serotype/genotype specificity or a lack of reactivity to typing monoclonal antibodies (MAbs) used in this study. Strains belonging to E1, E2, and E5 exhibited genotype G10,P6[1], G3, and G1 specificities and accounted for 19.0, 42.9, and 9.5% of the isolates, respectively. Though they possessed G10-type VP7, the E1 strains exhibited high reactivity with the G6-specific MAb, suggesting that the uncommon combination of the outer capsid proteins altered the specificity of the conformation-dependent antigenic epitopes on VP7. E3 and E4 strains accounted for 28.6% of the isolates and were untypeable. Sequence analysis of VP7 from E4 strains (Erv92 and Erv99) revealed that they represent a new VP7 genotype, G16. The detection of unexpected bovine rotavirus-derived G10,P6[1] reassortants, G1 serotype strains, and a new genotype (G16) strain in two distant farms reveals an interesting epidemiological situation and diversity of equine rotaviruses in India.

Full Genome-Based Classification of Rotaviruses Reveals a Common Origin between Human Wa-Like and Porcine Rotavirus Strains and Human DS-1-Like and Bovine Rotavirus Strains

“…More than 1,000 VP7-encoding nucleotide and deduced amino acid sequences, representing the 15 established G genotypes, were used for the analysis. The recently described equine strain ERV99, identified as a new G genotype (21), was revealed to be a G6 rotavirus strain with a high level of identity to NCDV (data not shown). The nucleotide-and amino acidbased phylogenetic dendrograms are shown in Fig.…”

Epidemiological Surveillance of P and G Genotypes of Group A Rotavirus Detected from Diarrheic Patients in Daejeon Region

“…Group A는 glycoprotein인 VP7과 proteasesensitive protein인 VP4의 항원적 성상에 의해 각각 G 아형와 P 아형으로 다시 분류된다. VP7의 중화항체반응 에 따라 지금까지 16개의 G형 (G1~G16)이 알려져 있으 며, VP7은 outer capsid의 대부분을 차지하고 있기 때문에 VP4에 비해 항원성이 높아 G 혈청형은 G 유전자형과 잘 일치된다고 보고되고 있다 (11,16,19). 그러나 VP4는 중화반응능이 약하며 특이적인 항체를 이용해서 검색하 여야 하기 때문에 중화반응에 의한 혈청학적 분류상 문 제가 많아 유전자 염기서열 분석에 의한 분류법을 많이 이용하고 있다 (11).…”

Genome Sequence of an Unusual Human G10P[8] Rotavirus Detected in Vietnam

“…The G10 rotavirus primarily causes acute gastroenteritis in cows, buffalo calves, and foals (3,4,6). The detection of G10 rotavirus from human feces recently was reported mainly in Africa and India (1,10).…”