Diversity of Campylobacter jejuni and Campylobacter coli Genotypes from Human and Animal Sources from Rio de Janeiro, Brazil

Aquino, Maria Helena Cosendey de; Filgueiras, Ana Luzia Lauria; Matos, Rita; Santos, Kátia Regina Netto dos; Ferreira, Tiago Osório; Ferreira, Mariana; Teixeira, Lúcia Martins; Tibana, Anita

doi:10.1016/j.rvsc.2009.08.005

Cited by 18 publications

(15 citation statements)

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“…The genetic diversity observed by RAPD-PCR in C. jejuni and C. coli was also reported by Aquino et al (2010), Madden et al (2007), Ridley et al (2008) and Workman et al (2008) in Campylobacter isolates from chickens, pigs and medical patients in Brazil, United Kingdom and Barbados. This genotypic variation may be due to over exposure to a source of contamination during the production process of chickens, or genetic changes in bacterial population after colonization (Workman et al 2008).…”

Section: Resultssupporting

confidence: 67%

Campylobacter jejuni and Campylobacter coli originated from chicken carcasses modulate their transcriptome to translate virulence genes in human cells

et al. 2019

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The aim was to determine the spread of genetically similar profiles of Campylobacter in chicken carcasses and evaluate their ability to produce transcripts for ciaB, dnaJ, p19 and sodB genes, before and after cultivation in Caco-2 cells. The strains used were isolated from 420 samples of chicken carcasses chilled and frozen ready for marketing. The species were identified by PCR-multiplex, the phylogeny was determined by RAPD-PCR and the presence of transcripts was performed by RT-PCR. We identified 74 (17.6%) of Campylobacter strains, being 55 (74.3%) C. jejuni and 19 (25.7%) C. coli. The phylogenetic relationship demonstrated heterogeneity between isolates of the same species, with absence of clones, indicating the high level of diversity of circulating genotypes. The gene transcription showed conflicting results before and after the culture in Caco-2 cell, so that before cultivation isolates showed greater capacity to transcribe genes related to survival and after the interaction with human cells, the strains showed higher potential to transcribe genes associated with virulence. The result of this study contributes to the understanding of how these seemingly fragile microorganisms are the most prevalent bacterial agents in human gastroenteritis.

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Section: Resultssupporting

confidence: 67%

Campylobacter jejuni and Campylobacter coli originated from chicken carcasses modulate their transcriptome to translate virulence genes in human cells

et al. 2019

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“…O sucesso da implementação de um método de tipagem molecular depende do seu poder discriminatório, que mede a capacidade de um método em atribuir um padrão molecular específico para um isolado, a proporção de linhagens distintas designadas por um método e a reprodutibilidade do método (Hunter, 1990;Maslow et al, 1993 Aquino et al, 2010;Ahmed et al, 2012;Wieczorek et al, 2013). …”

Section: Métodos De Tipagem De Campylobacterunclassified

“…No Brasil, o isolamento e o estudo de C. coli não são muito frequentes, o que dificulta avaliar a dimensão do envolvimento dessa bactéria como causadora de doença nos seres humanos e em animais, bem como, determinar o impacto de sua presença em alimentos e no meio ambiente (Scarcelli et al, 2005;Franchin et al, 2007;Aquino et al, 2010;Biasi et al, 2011).…”

Section: Especificamente 1 a 25% De Todas As Doenças Causadas Por Baunclassified

“…(Gomes et al, 2006;Franchin et al, 2010). Assim, há uma escassez de estudos que utilizaram técnicas moleculares para tipar linhagens de C. coli em nosso país (Scarcelli et al, 2005;Aquino et al, 2010 …”

Section: Especificamente 1 a 25% De Todas As Doenças Causadas Por Baunclassified

“…Além do uso veterinário, alguns ecossistemas como efluentes de hospitais humanos e de fazendas, sofrem com a liberação constante de antimicrobianos, o que pode modificar a atividade metabólica da microbiota desses, sendo que alguns tipos de antimicrobianos não são biodegradáveis, permanecendo longo período no ambiente (Martinez, 2009 Aquino et al, 2010;Ahmed et al, 2012;Wieczorek et al, 2013). O PFGE é uma das metodologias disponíveis mais discriminatórias para a genotipagem de Campylobacter spp.…”

Section: I S C U S S ã O | 66unclassified

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Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

Gomes¹

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Este espaço é dedicado àqueles que, de alguma forma, contribuíram para que esta dissertação fosse realizada. Não sendo viável nomeá-los a todos, há no entanto alguns a quem não posso deixar de manifestar o meu apreço e agradecimento sincero.Á Deus pela benção da vida, proteção e força para superar os obstáculos ao longo da minha jornada.À Profª Drª Juliana Pfrimer Falcão, pela orientação, pelo carinhoso apoio, partilha do saber e as valiosas contribuições para a realização desse trabalho. Acima de tudo, obrigada por continuar a acompanhar-me nesta jornada e por estimular o meu interesse pelo conhecimento. Agradeço pela confiança em mim depositada, pelo empenho, paciência e o exemplo profissional e moral durante todo esse período. Minha profunda admiração. Muito Obrigada por tudo! Á minha querida e amada Mãe pelo amor sem reservas, pelos momentos de carinho e por tornar a minha vida mais colorida. Ao meu Pai pela figura firme e segura que me empurra nos momentos mais difíceis, por tornar possível os meus sonhos e conquistas. Não tenho palavras para descrever esse Amor! Aos meus queridos irmãos Gabriela, Marcos, Carina e ao meu sobrinho João Vitor pelo incentivo, apoio e carinho. Vocês são parte de mim! Á aquele que não leva jeito com as palavras, mas tem um abraço que me acalma, um olhar que me alegra, um sorriso que conforta e um amor que me transforma. Alessandro, "Não me lembro mais qual foi nosso começo. Sei que não começamos pelo começo. Já era amor antes de ser"! strains; the flhA gene was detected in 11 (17.5%) strains; the dnaJ gene was detected in 10 (15.9%) strains; the pldA gene was detected in 7 (11.1%) strains ; the iamA gene was detected in three (4.8%) strains; the cdtC and docA genes were found in two (3.2%) strains; the cdtA and crsA were found in one (1.6%) strain and the ciaB, wlaN, virB11 and racR genes were not detected. Among the 63 strains studied, 42 were susceptible to all antimicrobials tested. Of the 21 resistant strains, 10 (15.9%) were resistante to tetracycline and doxaciclyne, six (9.5%) showed resistance to ciprofloxacin, and one (1.6%) was resistant to erythromycin. Only four (6.3%) strains were simultaneously resistant to at least two different classes of the antibiotics tested. The dendrogram of genetic similarity of Pulsed field gel electrophoresis (PFGE) grouped the 63 strains studied into two groups namely PFGE-A and PFGE-B with a genomic similarity of 44.9% among them. However, some strains isolated from humans, animals, the environment and food presented a high genotypic similarity above 80% and were subdivided into seven groups designated as PFGE-A1 to PFGE-A7. The dendrogram of genetic similarity of the SRV-flaA gene sequences grouped the strains studied into two groups namely SVR-A and SVR-B, with similarity above 83.1% among them. Besides, the deposit of the SVR sequences of the flaA gene in the online database showed that the alleles 30 and 1647 were the iv most frequently found and allowed the comparison between the strains studied with the alleles described in the d...

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Comparative clustering and genotyping of Campylobacter jejuni strains isolated from broiler and turkey feces by using RAPD-PCR and ERIC-PCR analysis

2018

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Diversity of Campylobacter jejuni and Campylobacter coli Genotypes from Human and Animal Sources from Rio de Janeiro, Brazil

Cited by 18 publications

References 9 publications

Campylobacter jejuni and Campylobacter coli originated from chicken carcasses modulate their transcriptome to translate virulence genes in human cells

Campylobacter jejuni and Campylobacter coli originated from chicken carcasses modulate their transcriptome to translate virulence genes in human cells

Caracterização molecular de linhagens de Campylobacter coli isoladas de origens diversas

Comparative clustering and genotyping of Campylobacter jejuni strains isolated from broiler and turkey feces by using RAPD-PCR and ERIC-PCR analysis

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