Diversity of culturable bacteria and occurrence of phytopathogenic species in bean seeds (Phaseolus vulgaris L.) preserved in a germplasm bank

Trapiello, Estefanía; González, Ana J.

doi:10.1007/s10722-012-9902-y

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…tessellarius causing leaf freckles and leaf spots in wheat ( Triticum aestivum ) (Carlson & Vidaver, 1982a, b). Recently, a new subspecies was described (González & Trapiello, 2012, 2014), Clavibacter michiganensis subsp. phaseoli , that infects bean ( Phaseolus vulgaris L.) and causes bacterial bean leaf yellowing.…”

mentioning

confidence: 99%

Seed-associated subspecies of the genus Clavibacter are clearly distinguishable from Clavibacter michiganensis subsp. michiganensis

Yasuhara-Bell

Alvarez

2015

International Journal of Systematic and Evolutionary Microbiology

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The genus Clavibacter contains one recognized species, Clavibacter michiganensis. Clavibacter michiganensis is subdivided into subspecies based on host specificity and bacteriological characteristics, with Clavibacter michiganensis subsp. michiganensis causing bacterial canker of tomato. Clavibacter michiganensis subsp. michiganensis is often spread through contaminated seed leading to outbreaks of bacterial canker in tomato production areas worldwide. The frequent occurrence of non-pathogenic Clavibacter michiganensis subsp. michiganensis-like bacteria (CMB) is a concern for seed producers because Clavibacter michiganensis subsp. michiganensis is a quarantine organism and detection of a nonpathogenic variant may result in destruction of an otherwise healthy seed lot. A thorough biological and genetic characterization of these seed-associated CMB strains was performed using standard biochemical tests, cell wall analyses, metabolic profiling using Biolog, and singlegene and multilocus sequence analyses. Combined, these tests revealed two distinct populations of seed-associated members of the genus Clavibacter that differed from each other, as well as from all other described subspecies of Clavibacter michiganensis. DNA-DNA hybridization values are 70 % or higher, justifying placement into the single recognized species, C. michiganensis, but other analyses justify separate subspecies designations. Additionally, strains belonging to the genus Clavibacter isolated from pepper also represent a distinct population and warrant separate subspecies designation. On the basis of these data we propose subspecies designations for separate non-pathogenic subpopulations of Clavibacter michiganensis: Clavibacter michiganensis subsp. californiensis subsp. nov. and Clavibacter michiganensis subsp. chilensis subsp. nov. for seed-associated strains represented by C55 T (5ATCC BAA-2691 T 5CFBP 8216 T) and ZUM3936 T (5ATCC BAA-2690 T 5CFBP 8217 T), respectively. Recognition of separate subspecies is essential for improved international seed testing operations.

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mentioning

confidence: 99%

Seed-associated subspecies of the genus Clavibacter are clearly distinguishable from Clavibacter michiganensis subsp. michiganensis

Yasuhara-Bell

Alvarez

2015

International Journal of Systematic and Evolutionary Microbiology

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“…Nevertheless, the other reports from Spain and Germany suggest that introduction could be linked to the import of infected seeds. Although the origin of these seeds was not reported, this hypothesis is supported by the observation that just one or two cultivars of the host were found to be affected (Sammer and Reiher, 2012;Trapiello and Gonz alez, 2012).…”

Section: Entrymentioning

confidence: 86%

“…The pathogen was first isolated in Spain from bean seeds in 2001, and subsequently found in 2005 in a bean field (cultivar Donna) in South Eastern Spain (Gonz alez et al, 2005). Seed lots of some local bean varieties preserved in a germplasm bank were also found to be infected, 10 years after their production in north-western Spain (Galicia), which the authors suggested to be probably due to contaminated foreign seeds (Trapiello and Gonz alez, 2012). Is the pest present in the EU territory?…”

Section: Pest Distribution In the Eumentioning

confidence: 99%

Pest categorisation of Curtobacterium flaccumfaciens pv. flaccumfaciens

Jeger¹,

Bragard²,

Candresse³

et al. 2018

EFS2

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Following a request from the European Commission, the EFSA Panel on Plant Health performed a pest categorisation of the seed-borne bacterium Curtobacterium flaccumfaciens pv. flaccumfaciens. The pest is regulated in Council Directive 2000/29/EC (Annex IIB) as a harmful organism whose introduction into, and spread within, the protected zones (PZ) of Greece, Portugal and Spain shall be banned if present on seeds of Phaseolus vulgaris and of Dolichos. The bacterium is widely distributed outside the EU and causes a systemic vascular disease (bacterial wilt of bean) as well as bacterial tan spot disease on soybean. The pest was sporadically recorded in several EU Member States in the past, but is currently not known to occur in the EU. The identity of the bacterium is well established and identification methods are available. The major host is common bean (Phaseolus vulgaris), but other crops and weeds are, or may be, hosts or play a role as reservoirs, with uncertainties. Seed transmission remains uncertain for minor and alternative host species. The main pathway for entry is seed. The role of other pathways (e.g. irrigation water and infected residues) is uncertain. Should the bacterium enter the EU (including the PZ), it may establish, spread and have an impact on its host crops. The use of healthy seeds is the most effective control measure. Curtobacterium flaccumfaciens pv. flaccumfaciens fits all the criteria assessed by EFSA to be regarded as a Union quarantine pest.

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