Diversity of endophytic and rhizosphere bacteria from pineapple (Ananas comosus) plant in semi-arid ecosystem

Putrie, Rahayu Fitriani Wangsa; Aryantha, I Nyoman Pugeg; Iriawati, Iriawati; Antonius, Sarjiya

doi:10.13057/biodiv/d210728

Cited by 7 publications

(5 citation statements)

References 44 publications

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“…All the isolates were maintained and analyzed for antifungal and antibacterial potentialities. Putrie et al (2020) found that the major microorganisms in the rhizospheric soil are actinobacteria. Also, Khamna et al (2009) demonstrated that microorganisms from the rhizosphere are most ideal for controlling plant pathogens that's why we have explored rhizospheric soil.…”

Section: Screening Of Antagonistic Isolatesmentioning

confidence: 99%

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

2020

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Abstract. Aouar L, Boukelloul I, Benadjila A. 2020. Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties. Biodiversitas 21: 5672-5683. To produce new bioactive substances of agricultural interest, extreme ecosystems can be a source of unexplored microorganisms. Accordingly, in this study, twenty-two actinobacteria strains were obtained from rhizospheric arid soils of palm groves collected from Biskra and El Oued in the Algerian Sahara. All isolates were examined for the in vitro antifungal potential towards phytopathogenic fungi: Aspergillus flavus, Verticillium dahlia, Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum as well as for their antibacterial property toward phytopathogenic bacteria: Streptomyces scabiei, Pectobacterium carotovorum and Agrobacterium tumefaciens. The three isolates (13%) that inhibited at least five pathogens were then selected, identified and assessed for their attributes to produce indole-3-acetic acid (IAA) and siderophores, to solubilize phosphate, and to antagonize Streptomyces scabiei in vivo. According to phylogenetic analysis performed with 16S rDNA sequence, chemotaxonomy and phenotypic characteristics, the strain SO1, which inhibited all tested pathogens, was assigned to Streptomyces flaveus. While, strains SO2 and SB1 were affiliated to Streptomyces enissocaesilis and Streptomyces albidoflavus, respectively. All strains produced IAA but only SO1 and SB1 were able to elaborate siderophores catecholate-type. Two strains SO1 and SO2 exhibited a capacity to solubilize phosphate and SO1 was able to suppress the pathogenic effect of Streptomyces scabiei on radish seedlings. The findings indicate that SO1 strain may reveal the potential for use as a biocontrol agent and plant growth promoter.

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Section: Screening Of Antagonistic Isolatesmentioning

confidence: 99%

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

2020

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“…However, the relationship between endophytes and rhizosphere microorganisms is still unclear. Current studies have shown that the microbiome of cotton changes with space and time and affects availability of soil nitrogen, thereby affecting the growth and reproduction of plant ( Zhang et al, 2011 ; Tian and Zhang, 2017 ; Moronta-Barrios et al, 2018 ; Lu et al, 2020 ; Putrie et al, 2020 ). Therefore, the changes in microbiome may affect healthy of cotton.…”

Section: Introductionmentioning

confidence: 99%

Differentiation and Variability in the Rhizosphere and Endosphere Microbiomes of Healthy and Diseased Cotton (Gossypium sp.)

Shi

Niu²,

Wang

et al. 2021

Front. Microbiol.

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The plant microbiome is a key determinant of health and productivity. However, it is still difficult to understand the structural composition of the bacterial and fungal microbiomes of diseased and healthy plants, especially the spatial dynamics and phylogenies of endophytic and rhizosphere microbial communities. We studied the differentiation and variability in the rhizosphere and endosphere microbiomes of healthy and diseased cotton from north and south of the Tianshan Mountains using the methods of PCR-based high-throughput sequencing and real-time quantitative PCR. The endophytic and rhizosphere bacterial abundances in the diseased plants were greater than those of healthy plants. The numbers of endophytic and rhizosphere fungi associated with diseased plants were greater than those associated healthy plants (p < 0.05). Endophytic and rhizosphere bacteria did not share common OTUs. The dominant rhizosphere bacteria were Proteobacteria (29.70%), Acidobacteria (23.14%), Gemmatimonadetes (15.17%), Actinobacteria (8.31%), Chloroflexi (7.99%), and Bacteroidetes (5.15%). The dominant rhizosphere fungi were Ascomycota (83.52%), Mortierellomycota (7.67%), Basidiomycota (2.13%), Chytridiomycota (0.39%), and Olpidiomycota (0.08%). The distribution of dominant bacteria in different cotton rhizosphere soils and roots differed, with the dominant bacteria Pseudomonas (15.54%) and Pantoea (9.19%), and the dominant fungi Alternaria (16.15%) and Cephalotrichum (9.10%) being present in the greatest numbers. At sampling points in different ecological regions, the total numbers of cotton endophytic and rhizosphere microbiome OTUs from southern to northern Xinjiang showed an increasing trend. There were significant differences in the composition and diversity of rhizosphere microbes and endophytes during the entire cotton growth period and in representative ecological regions (p < 0.01), whereas rhizosphere microbes and endophytes showed no significant differences among the four growth periods and in representative ecological regions. RB41, H16, Nitrospira, and Sphingomonas play important roles in the microbial ecology of cotton rhizosphere soil. Pseudomonas accounted for a large proportion of the microbes in the cotton rhizosphere soil. This study provides an in-depth understanding of the complex microbial composition and diversity associated with cotton north and south of the Tianshan Mountains.

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“…The major influences that the rhizospheric microorganisms have, nowadays, become an important tool to preserve plant health by an eco-friendly approach. It has been established that actinobacteria are the major microorganism in the rhizosphere and are the most ideal for controlling plant pathogens (Putrie et al, 2020). For these reasons, rhizospheric actinomycetes have been chosen to be explored for antagonists screening.…”

Section: In Vivo Bioassay Against S Scabies On Radish Seedlingmentioning

confidence: 99%

Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

et al. 2023

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Biocontrol is considered as an effective alternative to the application of agrochemicals, which are harmful to the environment, human, and animal health. In this study, twenty-six strains of actinomycetes were isolated from rhizospheric arid soil of the halophyte Atriplex halimus L. ῾Guettaf’ in Biskra province, Algeria. The six isolates that have inhibited at least three phytopathogenic fungi among the five tested (Fusarium oxysporum, Alternaria alternata, Fusarium solani, Aspergillus flavus and Botrytis cinerea) were selected, and have been tested in vitro against phytopathogenic bacteria (Pectobacterium carotovorum and Streptomyces scabies). They were also evaluated for their ability to hydrolyze phosphate, elaborate siderophores, produce indole-3-acetic acid (IAA), and to antagonize S. scabies in vivo (on radish seedlings). Based on the physicochemical analyses, soil samples were categorized as alkaline and extremely-saline. The antagonism results revealed varying antifungal potential among the selected isolates (Act11, Act16, Act17, Act18, Act23 and Act24), about 50% were able to inhibit the growth of F. solani and A. flavus, followed by 33.33% of those having antagonized F. oxysporum, while A. alternata was found to be the most sensitive. Only Act18 has antagonized S. scabies in vitro with an inhibition diameter zone of 19 ± 0.41 mm. However, in vivo trials showed that four isolates have counteracted S. scabies. Among them, Act18 and Act24 have significantly and positively affected the root surface (P = 0.0062) and prevented common scab. IAA was detected in all selected isolates with Act24 being the highest producer (77.45 μg mL−1). Additionally, degradation ability revealed that four isolates were able to hydrolyze phosphate while three exhibited the capacity of elaborating siderophores. The six isolates were assigned to Streptomyces genius according to their morphological, physiological and chemotaxonomical traits. Based on this study, Streptomyces sp. Act18 and Streptomyces sp. Act24 that tolerate 7.5% NaCl concentration, prevent common scab and exhibit some plant growth attributes, may be considered as promising biocontrol agents to be applied in arid and saline soils.

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Diversity of endophytic and rhizosphere bacteria from pineapple (Ananas comosus) plant in semi-arid ecosystem

Cited by 7 publications

References 44 publications

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

Differentiation and Variability in the Rhizosphere and Endosphere Microbiomes of Healthy and Diseased Cotton (Gossypium sp.)

Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

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