Diversity of endophytic bacterial communities in poplar grown under field conditions

Abstract

Bacterial endophytes may be important for plant health and other ecologically relevant functions of poplar trees. The composition of endophytic bacteria colonizing the aerial parts of poplar was studied using a multiphasic approach. The terminal restriction fragment length polymorphism analysis of 16S rRNA genes demonstrated the impact of different hybrid poplar clones on the endophytic community structure. Detailed analysis of endophytic bacteria using cultivation methods in combination with cloning of 16S rRNA genes amplified from plant tissue revealed a high phylogenetic diversity of endophytic bacteria with a total of 53 taxa at the genus level that included Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes. The community structure displayed clear differences in terms of the presence and relative proportions of bacterial taxa between the four poplar clones studied. The results showed that the genetic background of the hybrid poplar clones corresponded well with the endophytic community structure. Out of the 513 isolates and 209 clones identified, Actinobacteria, in particular the family Microbacteriaceae, made up the largest fraction of the isolates, whereas the clone library was dominated by Alpha- and Betaproteobacteria. The most abundant genera among the isolates were Pseudomonas and Curtobacterium, while Sphingomonas prevailed among the clones.

Diversity of endophytic bacteria in Caragana microphylla grown in the desert grassland of the Ningxia Hui Autonomous Region of China

“…Endophytic bacteria of plants colonize specific ecological niches in different tissues, which might be a reason for the diversity observed within the bacterial community in this study. This finding is consistent with those from other studies (Hallmann et al, 1997;Ulrich et al, 2008).…”

Impact of metagenomic DNA extraction procedures on the identifiable endophytic bacterial diversity in Sorghum bicolor (L. Moench)

“…These genera have previously been detected/isolated from tissues of other graminaceous and non-graminaceous plants including maize, sugarcane, rice, poplar, grapevine and sunflower (Pereira et al, 2011;Magnani et al, 2010;Sun et al, 2008;Ulrich et al, 2008;Compant et al, 2011;Ambrosini et al, 2012), thus indicating that these genera are probably ubiquitous in endophytic environments. This study also shows that the structures of sorghum stem and root communities are significantly different, and this could be explained by the differences in the selectivity of recruitment processes of microbes into each niche environment (Hardoim et al, 2008).…”

Characterization of endophytic bacteria associated with rose plant (Rosa damascena trigintipeta) during flowering stage and their plant growth promoting traits

“…Endophytic bacteria have been found in virtually every plant studied, however, these studies mainly focused on agricultural crops, economically important crops, and some trees (McInroy and Kloepper 1994; Ulrich et al 2008;Jha and Kumar 2009;Naik et al 2009). Of these, cotton, potato, rice, wheat, and sweet corn were extensively studied, whereas plant species growing in harsh conditions and having important officinal and industrial values such as Rose plant, which is growing on the crags of highaltitude mountains, have not been investigated with respect to their associated microbial community.…”