Diversity of endophytic fungi in Syzygium aqueum

Habisukan, Ummi Hiras; Elfita, Elfita; Widjajanti, Hary; Setiawan, Arum; Kurniawati, Alfia Rahma

doi:10.13057/biodiv/d220307

Cited by 15 publications

(20 citation statements)

References 29 publications

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“…The isolation process was carried out on a PDA medium because fungal cultures grew faster on this medium and several species might not have been isolated before. The diversity of endophytic fungi isolates was not only found in S. zeylanicum but was also found in the isolation of endophytic fungi from S. cumini (Hanin and Fitriasari 2019), S. aqeueum (Habisukan et al 2021), S. aromaticum (Shofiana et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Several genera of fungi isolated from the root bark of S. zeylanicum were found on other Syzygium plants, such as the genus Penicillium found on the leaves of Syzygium aqueum (Habisukan et al 2021). The genus Aspergillus is found in Syzygium aqueum (Habisukan et al 2021), Syzygium aromaticum (Shofiana et al 2015), and Syzygium paniculatum (Ploetz et al 2009). Penicillium brefeldianum has cytotoxic activity against HepG2 and MDA-MB-231 cells, this fungus had been isolated from the root of Pinellia ternate (Gao et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The endophytic fungi of Trichoderma species (SZR4 and SZR5 isolates) that are associated with plant roots can provide various benefits to their hosts such as cultural protection agents to control pathogens and promote plant growth (Cummings et al 2016) The root-associated Trichoderma endophytes have been found in several tropical plants (Cummings et al 2016) such as Syzygium aqueum (Habisukan et al 2021), Syzygium aromaticum (Mandla 2018). The endophytic fungus SZR7 identified as Chaetomium was also found in Syzygium aqueum (Habisukan et al 2021). Chaetomium is also an endophytic fungus from Curcuma wenyujin and produces chemical compounds such as chaetoglobosin, ergosterol, 2-methyl-3hydroxy indole, and chaetoglobosin X.…”

Section: Discussionmentioning

confidence: 99%

“…Purification was carried out by transferring the colonies to a new PDA medium with a single spore isolation method, then incubated at room temperature for 2 x 24 hours. The purified fungal colonies were re-cultured on PDA media as working culture (in Petri dishes) and stock cultures (in test tubes) (Fitriarni and Kasiamdari 2018;Hanin and Fitriasari 2019;Habisukan et al 2021).…”

Section: Isolation Of Endophytic Fungimentioning

confidence: 99%

“…The culture medium was extracted using ethyl acetate (1:1) three times. The ethyl acetate extract was separated from the culture medium by separating funnel and evaporated using a rotary evaporator (Deepika et al 2014;Habisukan et al 2021). The extract was concentrated using an oven at 40°C.…”

Section: Cultivation and Extractionmentioning

confidence: 99%

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Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite

et al. 2021

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Abstract. Syarifah, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite. Biodiversitas 22: 4572-4582. The decoction of the root bark of Syzygium zeylanicum has been used as traditional medicine, such as for treating pathogenic bacterial infections. Endophytic fungi that live in medicinal plant tissues have a high species diversity and biological activities correlate with their host. Therefore, this study aimed to explore the diversity of endophytic fungi from the root bark of S. zeylanicum and to determine the antibacterial activity of endophytic fungi and their secondary metabolites. In this study, we isolate and identify the endophytic fungi from the root bark of S. zeylanicum, continued by screening their antibacterial activity against two Gram-negative bacteria (Escherichia coli InaCCB5 and Salmonella thypi ATCC1048 and two Gram-positive bacteria (Staphylococcus aureus InaCCB4 and Bacillus subtilis InaCCB1204) by the Kirby-Bauer method. The fungal extract with the highest antibacterial activity proceeded with the isolation and determination of the structure of their bioactive compounds. The isolates were morphologically identified. Isolates that showed strong antibacterial activity were identified by molecular identification. Isolation of bioactive compounds was carried out by chromatographic techniques and the determination of the structure of pure chemical compounds was performed by the spectroscopic analysis. In total, there were 8 isolates of endophytic fungi were obtained from the root bark of S. zeylanicum, namely SZR1 – SZR8. SZR2 isolate has the highest antibacterial activity. Molecular identification through phylogenetic analysis showed that SZR2 isolate had high similarity with Penicillium brefeldianum. Isolation of bioactive compounds from SZR2 produced compound 1 in the form of light yellow crystals which showed strong antibacterial activity against S. typhi, E. coli, and B. subtilis with MIC values of 64 g/mL. Compound 1 was identified as p-hydroxybenzaldehyde, which was also obtained in its host. In conclusion, the endophytic fungus Penicillium brefeldianum produces similar secondary metabolites and antibacterial activity as its host plant.

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Section: Discussionmentioning

confidence: 99%