Diversity of extremely halophilic cultivable prokaryotes in Mediterranean, Atlantic and Pacific solar salterns: Evidence that unexplored sites constitute sources of cultivable novelty

Abstract

26The culturable fraction of aerobic, heterotrophic and extremely halophilic microbiota retrieved 27 from sediment and brine samples of eight sampling sites in the Mediterranean, Canary Islands

Moderate halophilic bacteria colonizing the phylloplane of halophytes of the subfamily Salicornioideae (Amaranthaceae)

“…Randomly selected colonies were analyzed by MALDI-TOF/MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry), as previously reported [65]. Groups of spectra clustering in the dendrograms were considered as operational taxonomic units (OTUs; see below).…”

“…OTUs selected from the P and N dendrograms were defined with a cut-off at the 720 and 750 distance levels, respectively. Several representatives of each OTU were selected for their DNA extraction, 16S rRNA gene amplification and sequencing, and phylogenetic reconstruction, as previously reported [65]. The sequences have been deposited at the EMBL repository under the accession numbers (LN651124-LN651155).…”

“…The sequences have been deposited at the EMBL repository under the accession numbers (LN651124-LN651155). OPUs (operational phylogenetic units; see below) were circumscribed by manually inspecting the resulting final tree [65].…”

“…PCR amplification was performed using bacterial primers GM3 and S (Supplementary Table S1), as previously reported [65]. A second short PCR (five cycles) was performed in a final volume of 25 L in triplicate in order to incorporate tags and a linker into the amplicon using a 1:25 dilution of the original products as templates, using the same conditions as the first PCR.…”

Halobium palmae gen. nov., sp. nov., an extremely halophilic archaeon isolated from a solar saltern

“…They are widely distributed in various hypersaline environments, from which they have been isolated. Solar salterns are one of the hypersaline environments inhabited by halophilic archaea and remain one of the primary sources of novel strains (Henriet et al, 2014;Viver et al, 2015). There are many solar salterns worldwide and natural salt is produced by slightly different methods at each site on a large or small scale.…”