Diversity of floricolous yeasts and filamentous fungi of some ornamental and edible fruit plants in Assiut area, Egypt

Abstract

The floricolous yeasts and filamentous fungi of 44 samples of flowers from both ornamental (20 samples) and edible fruit plants (24 samples) were evaluated. The general isolation medium DRBC supported more fungal species diversity in both flowers, than the xerophilic media (DG18 and MY50G). The highest numbers of fungal propagules were recovered on DG18 from flowers of ornamental plants, while the lowest on MY50G from flowers of edible plants. Yeasts constituted small proportion of propagules from the two flower types on the three media. Yeasts were represented by 18 genera and 26 species. Metschnikowia (3 species, from which M. reukaufii and M. viticola), Candida (4 species, from which C. riodocensis and C. vaccinia), Cryptococcus (C. albidus var. kuetzingii), Meyerozyma (M. guilliermondii), Naganishia (N. diffluens), Rhodotorula (2 spp., R. mucilaginosa), and Vishniacozyma (2 spp., V. carnescens) were infrequently encountered on the three media, beside Filobasidium (2 species), Galactomyces (G. candidus), Papiliotrema (P. flavescens), Pichia (P. kluyveri), and Sporidiobolus (S. metaroseus) which were recorded on two media. Some other yeast species were recovered only from one flower type but not from the other. Cladosporium (10 species) was the most common genus (100 % of samples from both types of flowers), accounting from 66.45 % to 87.25 % of total fungi. C. herbarum, C. cladosporioides, C. oxysporum, and C. sphaerospermum were recovered in high frequency from both types of flowers, but C. herbarum yielded the major proportion (61.23 % to 75.77 % of total fungi). Other filamentous fungi e.g. Alternaria (19 species, from which A. alternata and A. chlamydospora), Aspergillus (47 species, from which A. flavus, A. niger, and A. terreus), Penicillium (29 spp., P. chrysogenum and P. olsonii), Fusarium (12 spp., F. incarnatum, F. solani, and F. verticillioides), and Stemphylium (3 species, S. botryosum and S. sarrciniforme) were found contaminating all flowers on almost all isolation media.

A Snapshot Picture of the Fungal Composition of Bee Bread in Four Locations in Bulgaria, Differing in Anthropogenic Influence

“…Until now, Toxicocladosporium genus has never been reported to be associated with bee products and bees. Members of the genus were found in many different environments such as soils [56], marine waters [57], household air conditioners [58], bronchoalveolar lavage fluid [59], different plants [60,61], and even in insects-in the planthopper gut [62] and the Thitarodes larvae hemolymph [63]. However, members of this genus have never been established as strict pathogens of the plant and animal species where they thrive.…”

Fermentative Microbes of Khadi, a Traditional Alcoholic Beverage of Botswana

“…have been reported to be among the main non-conventional yeasts known to be involved in spontaneous alcoholic fermentations of wine, beer, tequila, mezcal and cachaça [25,26]. Although basidiomycetous yeasts have been isolated from fruits and fermented products [2,27], their role in khadi fermentation remains unknown. S. cerevisiae, a well-known fermenting yeast inhabiting sugar rich environment such as wild fruits, was only isolated from Letlhakane samples.…”

Mycotoxins in khadi, A Traditional Non-Cereal Based Alcoholic Beverage of Botswana

“…Other isolates of interest that were identified were C. sphaerospermum and C. cladosporioides , which are filamentous fungi previously isolated from flowers of edible fruit plants as well as being isolated from honey, dairy products and grapes prior to harvesting. 8 , 25 - 27 The Cladosporium species have been described in the past as mycotoxin-producing and possibly pathogenic fungi that are frequently found in outdoor settings. 28 The subsequent mycotoxin studies were necessary because the filamentous fungi were deemed to be a potential signal of mycotoxin presence in the khadi samples.…”