Diversity of food-borne <i>Bacillus</i>
 volatile compounds and influence on fungal growth

Chaves‐López, Clemencia; Serio, Annalisa; Gianotti, Andrea; Sacchetti, Giampiero; Ndagijimana, Maurice; Ciccarone, Claudio; Stellarini, A.; Corsetti, Aldo; Paparella, Antonello

doi:10.1111/jam.12847

Cited by 96 publications

(75 citation statements)

References 32 publications

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“…Some of the detected compounds have been reported previously in microbial fermentation broths, including those from actinomycetes and Streptomyces sp. For instance, 2(5H)-furanone (1) (Braun et al, 1995), 2-ethyl hexan-1-ol (2) (Sunesson et al, 1997), 1-octanol (3) (Gu et al, 2007; Chaves-López et al, 2015), 1-undecanol (4) (Liu et al, 2015), 2-octanol (5) (Spreafico et al, 1997), phenol, 2,5- bis (1,1-dimethylethyl)- (6) (Zhang et al, 2013; Nandhini, 2015), ethyl 4-ethoxybenzoate (7) (Sharma et al, 2010), 1-tetradecanol (8) (Nhi-Cong et al, 2010), cyclo( L -Pro- D -Ala) (9) , cyclo(Gly-Pro) (10) (Ser et al, 2015b), cyclo(Leu-Pro) (11) , cyclo(Pro-Phe) (12) (Jog et al, 2014; Sharma et al, 2016) and phenol, 2,2′-methylene bis [6-(1,1-dimethylethyl)-4-methyl-] (13) (Tan et al, 2015b). …”

Section: Discussionmentioning

confidence: 99%

Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties

et al. 2017

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Reactive oxygen species and other radicals potentially cause oxidative damage to proteins, lipids, and DNA which may ultimately lead to various complications including mutations, carcinogenesis, neurodegeneration, cardiovascular disease, aging, and inflammatory disease. Recent reports demonstrate that Streptomyces bacteria produce metabolites with potent antioxidant activity that may be developed into therapeutic drugs to combat oxidative stress. This study shows that Streptomyces sp. MUM212 which was isolated from mangrove soil in Kuala Selangor, Malaysia, could be a potential source of antioxidants. Strain MUM212 was characterized and determined as belonging to the genus Streptomyces using 16S rRNA gene phylogenetic analysis. The MUM212 extract demonstrated significant antioxidant activity through DPPH, ABTS and superoxide radical scavenging assays and also metal-chelating activity of 22.03 ± 3.01%, 61.52 ± 3.13%, 37.47 ± 1.79%, and 41.98 ± 0.73% at 4 mg/mL, respectively. Moreover, MUM212 extract was demonstrated to inhibit lipid peroxidation up to 16.72 ± 2.64% at 4 mg/mL and restore survival of Vero cells from H2O2-induced oxidative damages. The antioxidant activities from the MUM212 extract correlated well with its total phenolic contents; and this in turn was in keeping with the gas chromatography–mass spectrometry analysis which revealed the presence of phenolic compounds that could be responsible for the antioxidant properties of the extract. Other chemical constituents detected included hydrocarbons, alcohols and cyclic dipeptides which may have contributed to the overall antioxidant capacity of MUM212 extract. As a whole, strain MUM212 seems to have potential as a promising source of novel molecules for future development of antioxidative therapeutic agents against oxidative stress-related diseases.

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Section: Discussionmentioning

confidence: 99%

Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties

et al. 2017

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“…Many signaling molecules used for communication between microorganisms are VOCs (Fernando et al, 2005; Chaves-Lopez et al, 2015; Giorgio et al, 2015). However, our results strongly suggest that overproduction of fengycins when B. amyloliquefaciens S499 is co-cultured with R. variabilis is not related to VOCs emitted by the fungus nor to the detection of molecular patterns harbored at the cell surface since the two partners were not in physical contact.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of fungal VOCs on CLPs production was evaluated on MRE medium using two-compartments Petri plates (Fernando et al, 2005; Chaves-Lopez et al, 2015). In the first compartment, the bacterium was inoculated by depositing a 10 μl drop of a cell suspension adjusted to an OD of 0.1, whereas in the second compartment, a mycelial disk of freshly pre-cultured R. variabilis was deposited.…”

Section: Methodsmentioning

confidence: 99%

Stimulation of Fengycin-Type Antifungal Lipopeptides in Bacillus amyloliquefaciens in the Presence of the Maize Fungal Pathogen Rhizomucor variabilis

et al. 2017

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Most isolates belonging to the Bacillus amyloliquefaciens subsp. plantarum clade retain the potential to produce a vast array of structurally diverse antimicrobial compounds that largely contribute to their efficacy as biocontrol agents against numerous plant fungal pathogens. In that context, the role of cyclic lipopeptides (CLPs) has been well-documented but still little is known about the impact of interactions with other soil-inhabiting microbes on the expression of these molecules. In this work, we wanted to investigate the antagonistic activity developed by this bacterium against Rhizomucor variabilis, a pathogen isolated from diseased maize cobs in Democratic Republic of Congo. Our data show that fengycins are the major compounds involved in the inhibitory activity but also that production of this type of CLP is significantly upregulated when co-cultured with the fungus compared to pure cultures. B. amyloliquefaciens is thus able to perceive fungal molecules that are emitted and, as a response, up-regulates the biosynthesis of some specific components of its antimicrobial arsenal.

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“…Other research groups only occasionally identified single volatile compounds using authentic standards, while the remaining compounds were identified only by comparison with mass spectral databases (Tahir et al, 2017). In some cases it is not recognizable if volatiles were identified using authentic standards and/or libraries (Chaves-López et al, 2015). A clear assignment of an authentic standard confirmation was unfortunately missing.…”

Section: Identification Of Volatile Secondary Metabolitesmentioning

confidence: 99%

Diversity and Distribution of Volatile Secondary Metabolites Throughout Bacillus subtilis Isolates

Kai

2020

Front. Microbiol.

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Bacillus subtilis releases a broad range of volatile secondary metabolites, which are considered as long-and short distance infochemical signals mediating inter-and intraspecific processes. In addition, they often show antimicrobial or antifungal activities. This review attempts to summarize yet known volatile secondary metabolites produced and emitted by Bacillus subtilis isolates focusing on the structural diversity and distribution patterns. Using in vitro volatile-collection systems, 26 strains of B. subtilis isolated from different habitats were found to produce in total 231 volatile secondary metabolites. These volatile secondary metabolites comprised mainly hydrocarbons, ketones, alcohols, aldehydes, ester, acids, aromatics, sulfur-and nitrogen-containing compounds. Reviewed data revealed to a great extent isolate-specific emission patterns. The production and release of several volatile bioactive compounds was retained in isolates of the species B. subtilis, while volatiles without a described function seemed to be isolate-specifically produced. Detailed analysis, however, also indicated that the original data were strongly influenced by insufficient descriptions of the bacterial isolates, heterogeneous and poorly documented culture conditions as well as sampling techniques and inadequate compound identification. In order to get deeper insight into the nature, diversity, and ecological function of volatile secondary metabolites produced by B. subtilis, it will be necessary to follow well-documented workflows and fulfill state-of-the-art standards to unambiguously identify the volatile metabolites. Future research should consider the dynamic of a bacterial culture leading to differences in cell morphology and cell development. Single cell investigations could help to attribute certain volatile metabolites to defined cell forms and developmental stages.

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Diversity of food-borne Bacillus volatile compounds and influence on fungal growth

Cited by 96 publications

References 32 publications

Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties

Streptomyces sp. MUM212 as a Source of Antioxidants with Radical Scavenging and Metal Chelating Properties

Stimulation of Fengycin-Type Antifungal Lipopeptides in Bacillus amyloliquefaciens in the Presence of the Maize Fungal Pathogen Rhizomucor variabilis

Diversity and Distribution of Volatile Secondary Metabolites Throughout Bacillus subtilis Isolates

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