Diversity of fungal species in ancient parchments collections of the Archive of the University of Coimbra

Carvalho, H. Paiva de; Mesquita, Nuno; Trovão, João; Silva, J. Peixoto da; Rosa, Bárbara Santa; Albuquerque-Martins, Rui; Bandeira, Ana Maria Leitão; Portugal, António

doi:10.1016/j.ibiod.2015.12.001

Cited by 23 publications

(8 citation statements)

References 37 publications

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“…Although the isolates Zbf-S36, Zbf-T6, Zbf-S13, Zbf-S10, Zbf-L2, and Zbf-S15 were not accurately correlated to their corresponding species ( Figure 1 A), this is not surprising because Gibberella is the teleomorph of Fusarium . Besides, the anamorph phases of Bionectria , Diaporthe , and Botryosphaeria are Clonostachys , Phomopsis , and Dothiorella , respectively [ 20 , 21 , 22 ]. Based on a genetic standpoint, fungi with different generic names for the teleomorph and anamorph are actually the same fungus.…”

Section: Resultsmentioning

confidence: 99%

Biodiversity, Phylogeny, and Antifungal Functions of Endophytic Fungi Associated with Zanthoxylum bungeanum

Liu

et al. 2016

IJMS

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This study investigated the biodiversity, phylogeny, and antifungal activity of endophytic fungi isolated from Zanthoxylum bungeanum. A total of 940 isolates obtained were grouped into 93 morphotypes, 43 species, and 23 genera, which were authenticated by molecular identification based on rDNA internal transcribed spacer (ITS) sequence analysis. A high diversity of endophytic fungi from Z. bungeanum are observed with high species richness S (43), Margalef index D′ (6.1351), Shannon–Wiener index H′ (3.2743), Simpson diversity index Ds (0.9476), PIE index (0.9486), and evenness Pielou index J (0.8705) but a low dominant index λ (0.0524). Significant tissue specificity of the endophytic fungi was observed in Z. bungeanum, and the highest species richness and diversity indexes were obtained in the stem. Phylogenetic analyses of the 93 endophytic isolates were carried out by the neighbor-joining (NJ) method to demonstrate their evolutionary processes. Antifungal activities of endophytic fungi were assayed and eight endophytic isolates showed strong and long-lasting inhibition against host pathogenic fungi Fusarium sambucinum and Pseudocercospora zanthoxyli. Here, for the first time, we systematically demonstrate the biodiversity, phylogeny, and antifungal activity of endophytic fungi associated with Z. bungeanum and reveal the value of sampling different tissues of a given plant to obtain the greatest endophyte species diversity, which might offer a framework for further investigation and utilization of endophytic fungi as aunique source of interesting and useful bioactive compounds.

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Section: Resultsmentioning

confidence: 99%

Biodiversity, Phylogeny, and Antifungal Functions of Endophytic Fungi Associated with Zanthoxylum bungeanum

Liu

et al. 2016

IJMS

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“…(2016) P. collarifera Decaying Betula papyrifera log Canada: Quebec DAOMC:250755T MT026372 MT026480 MT018381 MT039019 MT009498 This study P. compacta Living bark of Alnus glutinosa Germany CBS:507.94T MH862480 MT026498 MT018411 MT039037 MT009516 Vu et al. (2019) , this study P. dimorphospora Parchment Portugal a209 KT898775 — — — — de Carvalho et al. (2016) Pear tree Greece AXL1SP1 KX881592 — — — — Markakis et al.…”

Section: Methodsmentioning

confidence: 97%

Mollisiaceae: An overlooked lineage of diverse endophytes

Tanney

Seifert

2020

Studies in Mycology

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Mollisia is a taxonomically neglected discomycete genus ( Helotiales, Leotiomycetes ) of commonly encountered saprotrophs on decaying plant tissues throughout temperate regions. The combination of indistinct morphological characters, more than 700 names in the literature, and lack of reference DNA sequences presents a major challenge when working with Mollisia . Unidentified endophytes, including strains that produced antifungal or antiinsectan secondary metabolites, were isolated from conifer needles in New Brunswick and placed with uncertainty in Phialocephala and Mollisia , necessitating a more comprehensive treatment of these genera. In this study, morphology and multigene phylogenetic analyses were used to explore the taxonomy of Mollisiaceae , including Mollisia , Phialocephala , and related genera, using new field collections, herbarium specimens, and accessioned cultures and sequences. The phylogeny of Mollisiaceae was reconstructed and compared using the nuc internal transcribed spacer rDNA (ITS) barcode and partial sequences of the 28S nuc rDNA (LSU) gene, largest subunit of RNA polymerase II ( RPB1 ), DNA topoisomerase I ( TOP1 ), and the hypothetical protein Lipin/Ned1/Smp2 ( LNS2 ). The results show that endophytism is common throughout the Mollisiaceae lineage in a diverse range of hosts but is infrequently attributed to Mollisia because of a paucity of reference sequences. Generic boundaries within Mollisiaceae are poorly resolved and based on phylogenetic evidence the family included species placed in Acephala , Acidomelania , Barrenia , Bispora , Cheirospora , Cystodendron , Fuscosclera , Hysteronaevia, Loramyces, Mollisia, Neopyrenopeziza , Obtectodiscus, Ombrophila, Patellariopsis , Phialocephala , Pulvinata , Tapesia (= Mollisia ), and Trimmatostroma. Taxonomic novelties included the description of five novel Mollisia species and five novel Phialocephala species and the synonymy of Fuscosclera with Phialocephala , Acidomelania with Mollisia , and Loramycetaceae with Mollisiaceae .

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“…Aureobasidium, Cladosporium, and Penicillium-were frequently isolated from historical parchment documents (Kraková et al, 2012;Gutarowska, 2016;Lech, 2016;Paiva de Carvalho et al, 2016). Apart from parchment-specific microorganisms, fungi of the genus Paecilomyces usually occur on surfaces of historical objects made of leather (Strzelczyk, 2004).…”

Section: Discussionmentioning

confidence: 99%

Application of a Medical Diode Laser (810 nm) for Disinfecting Small Microbiologically Contaminated Spots on Degraded Collagenous Materials for Improved Biosafety in Objects of Exceptional Historical Value From the Auschwitz-Birkenau State Museum and Protection of Human Health

2020

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The research aim was to optimize the operating parameters of a diode laser irradiation for the effective disinfection of degraded collagenous materials. Historical leather shoes stored at the Auschwitz-Birkenau State Museum in Oświęcim (Poland) were the main study objects. Surfaces of contaminated small spots occurring on the degraded materials were sampled with moistened swabs and microbiologically examined using the molecular techniques MALDI-TOF MS, 16S rRNA, and NGS sequencing. The surfaces were colonized by bacteria with 106 CFU/100 cm2 and 104 CFU/100 cm2 by fungi, on average. Microorganisms of the genera Bacillus and Penicillium were predominant. The effectiveness of the laser treatment was assessed for the new and degraded collagenous materials against isolated environmental strains using four variants of exposure time and number of repetitions. 0.3 W/CW 2 × 2 min variant was the most effective and also did not noticeably change the color of the treated samples. The variant caused a reduction in the numbers of microorganisms by 96–100%. After 1 month, four types of leather were subjected to comprehensive physico-chemical analyses. SEM and FTIR techniques confirmed that laser irradiation in the selected optimal variant did not affect the surface morphology and collagen structure, while XPS technique enabled detection of subtle changes in non-historical protective coatings on the surfaces of tested degraded historical materials.

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Diversity of fungal species in ancient parchments collections of the Archive of the University of Coimbra

Cited by 23 publications

References 37 publications

Biodiversity, Phylogeny, and Antifungal Functions of Endophytic Fungi Associated with Zanthoxylum bungeanum

Biodiversity, Phylogeny, and Antifungal Functions of Endophytic Fungi Associated with Zanthoxylum bungeanum

Mollisiaceae: An overlooked lineage of diverse endophytes

Application of a Medical Diode Laser (810 nm) for Disinfecting Small Microbiologically Contaminated Spots on Degraded Collagenous Materials for Improved Biosafety in Objects of Exceptional Historical Value From the Auschwitz-Birkenau State Museum and Protection of Human Health

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