Diversity of Gastrointestinal Nematodes in Domestic Ruminants of Uzbekistan

Abramatov, Makhamadi

doi:10.17582/journal.pjz/20210629120602

PJZ

2022

DOI: 10.17582/journal.pjz/20210629120602

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Diversity of Gastrointestinal Nematodes in Domestic Ruminants of Uzbekistan

Makhamadi Abramatov¹

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2023

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Cited by 1 publication

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Identification of nematodes of the genus Teladorsagia parasites of ruminants with the help of species-specific markers based on ITS2 rDNA

Ibrokhimov,

Kuchboev,

Amirov

et al. 2023

E3S Web Conf.

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The present study delves into a methodological framework aimed at establishing species-specific markers via the utilization of sequences derived from the internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA. This method, in conjunction with polymerase chain reaction (PCR) testing, serves as a diagnostic tool for discerning species belonging to the genus Teladorsagia Andreeva et Satubaldin, 1954. These species, constituents of the subfamily Ostertagiinae (Nematoda: Trichostrongylidae), exhibit wide distribution within the gastrointestinal tracts of ruminants across the geographic expanse of Uzbekistan. The heart of this endeavor is the development of species-specific primers, a pioneering creation in its own right. These primers are crafted using sequences emanating from the ITS2 region of the ribosomal DNA, an innovative approach that facilitates the precise identification of morphospecies within the Teladorsagia genus. Notably, the primers exhibit a nucleotide length of 153 base pairs, an attribute instrumental in their capacity to accurately distinguish and diagnose eggs and larvae of three distinct morphspecies: T. circumcincta, T. trifurcata, and T. davtiani. The potential implications of this method are significant, with ramifications reverberating across the field of veterinary diagnostics. Through the application of these primers, practitioners and researchers alike can effectively ascertain the presence of specific Teladorsagia morphospecies in ruminant animals. This holds the promise of not only enhancing diagnostic precision but also contributing to the broader comprehension of the prevalence and distribution of these nematode species within the local ruminant populations.

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Identification of nematodes of the genus Teladorsagia parasites of ruminants with the help of species-specific markers based on ITS2 rDNA

Ibrokhimov,

Kuchboev,

Amirov

et al. 2023

E3S Web Conf.

Self Cite

View full text Add to dashboard Cite

show abstract

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Diversity of Gastrointestinal Nematodes in Domestic Ruminants of Uzbekistan

Cited by 1 publication

References 27 publications

Identification of nematodes of the genus Teladorsagia parasites of ruminants with the help of species-specific markers based on ITS2 rDNA

Identification of nematodes of the genus Teladorsagia parasites of ruminants with the help of species-specific markers based on ITS2 rDNA

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