Diversity of marine yeasts with high protein content and evaluation of their nutritive compositions

Abstract

A total of 327 yeast strains from seawater, sediments, mud of salterns, guts of the marine fish and marine algae were obtained. After crude protein of the yeasts was estimated by the method of Kjehldahl, we found that eight strains of the marine yeasts grown in the medium with 20 g l−1 glucose contained more than 30.4 g protein per 100 g of cell dry weight. The results of routine identification and molecular methods show that they belong to Metschnikowa reukaui, Cryptococcus aureus, Aureobasidium pullulan, Yarrowia lipolytica and Hanseniaspora uvarum, respectively. With the exception of Aureobasidium pullulans 4#2 with nucleic acid of 7.7% (w/w), all other yeast strains contained less than 5.0% (w/w) of nucleic acid. Analysis of fatty acids shows that all the yeast strains tested had a large amount of C18:0 and C18:1 fatty acids while analysis of amino acids indicates that the yeast strains tested had a large amount of essential amino acids, especially lysine and leucine which are very important nutritive components for marine animals.

The Multiple and Versatile Roles of Aureobasidium pullulans in the Vitivinicultural Sector

“…pullulans has not been explored for its use as a source of SCP, but fungi proteins have more advantages than those obtained from bacteria and algae [89]. Work from Chi et al [90] showed that A. pullulans isolates had high levels of nucleic acid components and essential amino acids. Such properties could be helpful in wine fermentation if these components can be used for feeding the alcohol-and aroma-producing yeasts.…”

Su Ürünleri̇ İşleme Sanayi̇ Atiklarindan Üreti̇len Si̇lajin Ki̇myasal Ve Mi̇krobi̇yoloji̇k Kompozi̇syonunun Araştirilmasi Üzeri̇ne Bi̇r Çalişma

“…When the state of the herring meal and the processing wastes silage in the 30th day are compared in terms of amino acid composition and the content of essential amino acids, valine, methionine and phenylalaline were found to be much lower when compared to those in the fish meal, threonine and lysine were high, while histidine, isoleucine and leucine were found to be similar (Table 3). As the amino acid index in the study was above 0.95 for both of the silages (Table 1 and 2), it was concluded that the silages contain high quality protein [25]. It is believed that the silage will be a raw material with high quality protein that could be an alternative to the fish meal when the amount of protein deficit of the silage is supplemented by enrichers.…”

Nutritional quality of amino acid in farmed, farm‐aggregated and wild Axillary seabream ( Pagellus acarne ) with implications to Human Health

“…Samples were placed in the flask of the rotary evaporator, and after the water bath was adjusted to 65°C, the hydrochloric acid was evaporated. Then, the remaining samples were taken up in dilution solution with citrate-sodium citrate buffer (0.1 M, pH 2.2) and became ready for amino acid analysis (Chi et al, 2008;Srivastava, Hamre, Stoss, Chakrabarti, & Tonheim, 2006).…”