Diversity of Plant Actin–Myosin Systems

Haraguchi, Takeshi; Duan, Zhongrui; Tamanaha, Masanori; Ito, Kodai; Tominaga, Motoki

doi:10.1007/978-3-030-33528-1_4

Cited by 7 publications

(7 citation statements)

References 46 publications

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“…Although acto-myosin-mediated vesicle transport has been described and quantified for many cell types, the mechanisms underlying the specificity of the vesicular transport in plants have remained largely unknown. Haraguchi et al 24 proposed an Long-distance organelle transport in plant cells relies mostly on actin-myosin-based mechanisms and occurs typically on bundles of actin filaments. Some organelles may be shuttled via a kinesinmicrotubule-based mechanism.…”

Section: Cytoskeletal Coordination Of Cell Wall Assemblymentioning

confidence: 99%

Cytoskeletal regulation of primary plant cell wall assembly

et al. 2021

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Section: Cytoskeletal Coordination Of Cell Wall Assemblymentioning

confidence: 99%

Cytoskeletal regulation of primary plant cell wall assembly

et al. 2021

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“…As key transcription factors, CAMTAs appear to be near the top of the signaling hierarchy in abiotic and biotic stress-response pathways as well as playing key roles in development (Finkler et al ., 2007; Kim et al ., 2009; Iqbal et al ., 2020). Myosins are essential motor proteins that function in concert with the actin cytoskeleton to control cell and nuclear shape, cargo transport, cell division, and overall growth (Golomb et al ., 2008; Buschmann, 2018; Haraguchi et al ., 2019; Brueggeman et al ., 2022). We recently provided evidence that CML13 and CML14 function as novel myosin light chains (Symonds et al ., 2023), consistent with reports showing in vivo association of CML13 and CML14 with myosins (Van Leene et al ., 2022; Teresinski et al ., 2023).…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis CML13 and CML14 Have Essential And Overlapping Roles In Plant Development

Symonds,

Teresinski,

Hau

et al. 2023

Preprint

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Calmodulin-like proteins (CaM-like; CML) are the largest family of calcium-binding proteins in plants, yet the functions of most CMLs are unknown. Arabidopsis CML13 and CML14 are closely related paralogs that interact with the isoleucine-glutamine (IQ) domains of myosins, IQ-domain (IQD) proteins, and CaM-activated transcription factors (CAMTAs). Here, we explored the physiological roles of CML13 and CML14 during development by using dexamethasone-inducible RNA silencing to suppress either CML13 or CML14 transcript levels. In the absence of inducible suppression, CML13- and CML14-RNA-interference lines were indistinguishable from WT plants throughout development. In contrast, induction of silencing treatment led to rapid increases in RNA-hairpin production that correlated with a targeted reduction in CML13 or CML14 transcript levels and a range of developmental and morphological effects. RNA suppression treatment did not impair the germination of CML13- or 14-RNA-interference lines, but these seedlings were chlorotic, displayed high mortality, and failed to achieve seedling establishment. Induced RNA suppression of mature plants led to reduced silique length, shorter roots, and rapid leaf senescence in CML13- and 14-RNA-interference plants. Plants induced for RNA suppression at 2 weeks post-germination exhibited a much stronger phenotype than treatment of 3-, 4-, or 5-week-old plants. Collectively, our data indicate that both CML13 and CML14 are essential for normal development and function across a broad range of tissues and developmental stages.

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“…Myosin have structurally variable features, such as sequence divergence, gene duplications, length and domain architecture that may explain the ongoing need for a comprehensive phylogenetic analysis of such a prolific family ( Allaby and Woodwark, 2004 ; De Souza et al., 2018 ). Myosin is usually classified based on their motor domain sequences ( Haraguchi et al., 2019 ; Kijima et al., 2021 ). As it is well-documented that strongly supported phylogenetic clustering analysis and conserved domain organizations define new classes, a concise nomenclature of multiple members within these classes has not yet been developed.…”

Section: Discussionmentioning

confidence: 99%