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Surra is a non-cyclic parasitic disease caused by Trypanosoma evansi (T. evansi) and spread by biting flies. The disease has a severe impact on camel health, productivity, and market value, posing a significant threat to food safety and the economy. In a cross-sectional study, 370 blood samples were collected from camels in three Egyptian governorates. Samples were tested using parasitological (thin blood smear (TBS)), card agglutination test for T. evansi (CATT), and PCR to estimate the prevalence of T. evansi infection. Overall, the prevalence of T. evansi among examined camels was 17.3%, 18.9% and 22.7% using TBS, CATT and PCR methods, respectively. The risk of T. evansi infection in older camels (> 10 years) is higher than that in young ones (odds ratio (OR) = 9; 95% CI: 3.5–23.1), particularly during spring (OR = 2.5; 95% CI: 1.1–5.7). Furthermore, females and poor conditioned camels were 2.6 and four times more likely to get infection than males and good conditioned camels, respectively. The level of agreement between diagnostics tests were perfect kappa (> 0.83). Moreover, CATT showed higher sensitivity (0.83; 95% CI: 0.74–0.91) than TBS (0.76; 95% CI: 0.66–0.85) and both had perfect specificity (100%). In conclusion, our findings revealed a high rate of T. evansi infection in camels from the three Egyptian governorates. The CATT is a good test for routine use in control program of trypanosomiasis in camels.

Within their natural habitat, plants are subjected to abiotic stresses that include heat stress. In the current study, the effect of 4 h, 24 h, and 48 h of heat stress on Tetraena propinqua ssp. migahidii seedling’s protein profile and proteomic analyses were investigated. Total soluble protein SDS-PAGE (Sodium dodecyl sulfate–polyacrylamide gel electrophoresis) profile showed 18-protein bands, the newly synthesized protein band (with molecular weights 86.5, 30.2 and 31.4 KD) at 24 h of heat stress and 48 of normal conditions. Proteomic analysis showed that 81 and 930 targets are involved in gene and protein expression respectively. At 4 h, 57 genes and 110 proteins in C4 reached 56 genes and 173 proteins in T4. At 24 h, 63 genes and 180 proteins in C24 decreased to 54 genes and 151 protein in T24. After 48 h, 56 genes and 136 proteins in C48 increased to 64 genes and 180 proteins in T48. The genes and proteins involved in transcription, translation, photosynthesis, transport, and other unknown metabolic processes, were differentially expressed under treatments of heat stress. These findings provide insights into the molecular mechanisms related to heat stress, in addition to its influence on the physiological traits of T. propinqua seedlings. Heat stress-mediated differential regulation genes indicate a role in the development and stress response of T. propinqua . The candidate dual-specificity genes and proteins identified in this study paves way for more molecular analysis of up-and-down-regulation.

Introduction Beta-thalassemias are a group of inherited blood disorders caused by reduced or absent synthesis of beta chain of hemoglobin resulting in variable phenotypes ranging from clinically asymptomatic individuals to severe anemia symptoms. The objective of this study is to screen for the whole beta gene globulin and the LCR region and its clinical relevance in β-Thalassemia patients. Methods In this study, we collected 140 blood patients' samples with beta-thalassemia from different areas of Saudi Arabia. DNA was then extracted then the molecular scanning for the whole β-globin gene and the Locus control region (β-LCR) for patients' samples, was run using PCR. Results Sixty one mutations found in this study, including 22 new mutations not recorded in the database before. These deletions including: (*C-1960-1961 ca/-- del in hbb5) and (*c-519C A het in hbb9). Interestingly, the highest percentage in gene deletion occurred in exon 03A by ∼33% of the samples, while the highest percentage in gene addition of the gene occurred in exon 03B by ∼25%. Conclusion This study was unique to show several new mutations that would help in diagnosis and treatment. These results should be taken further to set up better management strategies to improve outcomes.