Heat-Induced Protein and Superoxide Dismutase Changes in Wild Tetraena propinqua ssp. Migahidii Seedlings

Differential expression gene/protein contribute to heat stress-responsive in Tetraena propinqua in Saudi Arabia

“…Seeds of three plants were used for three germination experiments. Fifty (50) seeds were germinated by placing in a Petri dish, moistened with 5 mL of distilled water, and incubated at 25 °C (C; control) and 40 °C (T; heat stress) for 4, 24, and 48 h ( Alafari and Abd-Elgawad, 2021 ). The samples were collected for extraction.…”

“…Protein was extracted by phosphate buffer ( Alafari and Abd-Elgawad, 2021 ). Supernatants were used for total soluble proteins SDS-PAGE profile and proteomics analysis.…”

“…Protein extract samples were mixed with loading buffer and 10 µl samples were loaded onto a gel in a Bio-Rad apparatus according to Laemmli ( Alafari and Abd-Elgawad, 2021 , Laemmli, 1957 ). The gel was removed from the apparatus gently and placed in a plastic tank, and covered with a fixing solution of 30% ethanol, 10% acetic acid overnight, followed by rinsing briefly in 5% acetic acid, then treated with a sensitizer working solution of 25 µl composed of 12.5 mL Sensitizer and 12.5 mL water and stained using “Pierce Silver Stain Kit” (Catalog number: 24612), using silver stain enhancer (0.125 mL of enhancer and 12.5 mL stain).…”

“…The plant's response to these stresses are combinations of changes in various processes ( Cramer et al, 2011 ). Among the common abiotic stresses, high temperature is considered a major factor that substantially affects plant growth and developmental processes ( Alafari and Abd-Elgawad, 2021 , Rodríguez Graña and del Soengas Fernández, 2015 ).…”

“…In the previous study, the effect of heat stress on three populations of Tetraena propinqua ssp. Migahidii caused protein degradation and conjointly induced expression of new synthesized HSPs throughout heat acclimatization in total soluble protein and superoxide dismutase may be related to heat injury and the improved thermotolerance in early hours of germination ( Alafari and Abd-Elgawad, 2021 ). In the present study, we examine the influence of heat stress on protein expression using proteomic methods in T. propinqua seedlings exposed to different durations of high-temperature stress, to identify proteins and genes closely related to heat stress, which enables T. propinqua to adapt to heat tolerance in Saudi Arabia.…”