Diversity of rhizobia nodulating Lotus corniculatus grown in northern and southern regions of Uruguay

Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus

“…T was unable to reproduce nodules in Lotus corniculatus and we failed to amplify the nodC gene with the primers described by Laguerre et al (2001) that allowed the amplification of nodC genes of Mesorhizobium strains from Lotus corniculatus isolated in the same region as our strain (Sotelo et al, 2011).…”

“…The root nodules were washed several times with sterile distilled water and surfacesterilized for 1 min in 90 % (v/v) ethanol followed by 3 min in 4 % (v/v) NaClO, then washed three times in sterile distilled water, as previously described (Sotelo et al, 2011). The nodules were then crushed using a sterile glass : 10 g mannitol, 1 g yeast extract, 0.2 g K 2 HPO 4 , 0.2 g MgSO 4 .…”

“…Colonies were white, mucoid, translucent and convex on this medium. Reinfection experiments were performed in Lotus corniculatus, as previously described (Sotelo et al, 2011). Strain S658…”

“…Although the species originated in temperate areas of Europe and North Africa, it has spread throughout the world as a result of cultivation (Díaz et al, 2005). Nowadays Lotus corniculatus is the most widely used legume in sown pastures in Uruguay, where it establishes symbiosis with Mesorhizobium (Sotelo et al, 2011).…”

Diversity pattern of nitrogen fixing microbes in nodules of <i>Trifolium arvense</i> (L.) at different initial stages of ecosystem development

“…Furthermore, it has been often postulated that the inter-and intra-species diversity of rhizobia, which are able to form nodules with selected legumes, strongly depend on environmental conditions like soil pH and water content or biogeography. For example, it was shown that R. etli as microsymbiont of beans can be replaced by R. tropici under acidic conditions (Anyango et al, 1995;Sadowsky and Graham, 2006) and that diversity of rhizobia infecting chickpea (Alexandre et al, 2009) or Lotus corniculatus (Sotelo et al, 2011) depends on the investigated biogeographic region. Moreover, annual legumes like T. arvense face the problem that the symbiosis must be established each year again (Sessitsch et al, 2002).…”