Diversity of the FcR- and KIR-related genes in an amphibian Xenopus

Guselnikov, Sergey; Ramanayake, Thaminda; Robert, Jacques; Taranin, Alexander V.

doi:10.2741/3234

Cited by 12 publications

(9 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It will be interesting to determine whether in Xenopus, like their mammalian counterparts, there is a sub-population of these cell types that express invariant TCR α chains that interact with conserved moieties of cell surface MHC molecules (59). Additionally, the recent characterization of the arsenal of FcR- and KIR-like activating and inhibitory receptor genes in Xenopus laevis (reviewed by 60 in this special issue) provides more tools with which to study immune molecules expressed on lymphocytes and their possible contribution to anti-tumor immunity. Therefore, our model offers the opportunity to examine a heterogeneous pool of anti-tumor effector cells conserved for over 350 million years of evolution, and characterize the conditions under which they exert their functions.…”

Section: Discussionmentioning

confidence: 99%

Tumorigenesis and anti-tumor immune responses in Xenopus

Goyos

Robert²

2009

Front Biosci

View full text Add to dashboard Cite

Despite intense study, the role of the immune system in detecting (immunosurveillance), controlling and remodeling (immunoediting) neoplasia remains elusive. We present here a comparative view of the complex interactions between neoplasia and the host immune system. We provide evidence, in the amphibian Xenopus laevis, consistent with an evolutionarily conserved and crucial role of the immune system in controlling neoplasia, which involves a striking variety of anti-tumoral immune effectors including conventional CTLs, classical MHC class Ia unrestricted CTLs (CCU-CTLs) that interact with nonclassical MHC class Ib molecules, CD8 NKT-like cells and NK cells. We also review the tumors found in X. laevis with an emphasis on thymic lymphoid tumors and a rare ovarian dysgerminoma. Finally, we consider the use of X. laevis for in vivo study of tumorigenesis. Given our current knowledge, the experimental systems already established in X. laevis, and the rapid accumulation of genetic resources for the sister species Silurana (Xenopus) tropicalis, it is our conviction that these species provide an ideal alternative to the murine system for studying tumorigenesis and tumor immunity.

show abstract

Section: Discussionmentioning

confidence: 99%

Tumorigenesis and anti-tumor immune responses in Xenopus

Goyos

Robert²

2009

Front Biosci

View full text Add to dashboard Cite

show abstract

“…Most divergent Xenopus sequences are comparable with those between amphibian and mammalian sequences. This observation suggests that the diversification of the amphibian FcR‐related molecules started long ago (131).…”

Section: Itims In Amphibiansmentioning

confidence: 92%

“…External domain comparisons also conclude to independent evolutionary histories, even when domain homology has been assigned. To obtain more information about the evolutionary history and the origins of the FcR and KIR families, it will be necessary to identify and characterize members of these families in reptiles, amphibians, bony and cartilaginous fish (131).…”

Section: Itims In Birdsmentioning

confidence: 99%

See 1 more Smart Citation

Immunoreceptor tyrosine‐based inhibition motifs: a quest in the past and future

Daëron

Jaeger

Pasquier

et al. 2008

Immunological Reviews

321

284

View full text Add to dashboard Cite

Since an immunoreceptor tyrosine-based inhibition motif (ITIM) was first identified in the intracytoplasmic domain of Fc gammaRIIB, ITIMs have been found in a large number of inhibitory molecules that were shown to negatively regulate cell activation. Due to their wide tissue distribution and to the variety of their extracellular ligands, ITIM-containing molecules are involved in the control of a large spectrum of biological functions, mostly but not exclusively related to immunity. On the basis of sequence comparison, ITIMs were structurally defined as 6-amino acid sequences containing a tyrosine (Y) with loosely conserved N-terminal (Y-2) and C-terminal (Y+3) residues. Molecular analysis of signaling events demonstrated that when coaggregated with activating receptors, ITIMs are phosphorylated by Src-family tyrosine kinases, which enables them to recruit Src homology 2 domain-containing phosphatases that antagonize activation signals. Because ITIM-dependent negative regulation seems to be a fundamental regulatory mechanism, both in rodents and in humans, and because it can be used either as a target or as a powerful tool in various diseases, we undertook (i) a genome-wide search of potential novel ITIM-containing molecules in humans, mice, frogs, birds, and flies and (ii) a comparative analysis of potential ITIMs in major animal phyla, from mammals to protozoa. We found a surprisingly high number of potential ITIM-containing molecules, having a great diversity of extracellular domains, and being expressed by a variety of immune and non-immune cells. ITIMs could be traced back to the most primitive metazoa. The genes that encode ITIM-containing molecules that belong to the immunoglobulin superfamily or to the C-lectin family seem to derive from a common set of ancestor genes and to have dramatically expanded and diverged in Gnathostomata (from fish to mammals).

show abstract

“…The same Ig domain subtypes D1-D5 that are present in human FCRLs are also present in Xenopus XFL; in addition, some XFLs have a sixth Ig domain subtype that may be Xenopus specific. In XFLs, the D3 Ig domain is frequently found repeated and the longest XFL has an extracellular region consisting of D1-D2-D3-D1-D2, followed by six D3 domains (67,68).…”

Section: Discussionmentioning

confidence: 99%

A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus

Taylor

Moulana

Stuge

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

Channel catfish, Ictalurus punctatus, leukocyte immune type receptors (LITRs) represent a multigene family that encodes Ig superfamily proteins that mediate activating or inhibitory signaling. In this study, we demonstrate the use of mAb CC41 to monitor viral cytotoxic responses in catfish and determine that CC41 binds to a subset of LITRs on the surface of catfish clonal CTLs. Homozygous gynogenetic catfish were immunized with channel catfish virus (CCV)–infected MHC-matched clonal T cells (G14D-CCV), and PBL were collected at various times after immunization for flow cytometric analyses. The percentage of CC41+ cells was significantly increased 5 d after primary immunization with G14D-CCV and at 3 d after a booster immunization as compared with control fish only injected with G14D. Moreover, CC41+ cells magnetically isolated from the PBL specifically killed CCV-infected targets as measured by 51Cr release assays and expressed messages for CD3γδ, perforin, and at least one of the CD4-like receptors as analyzed by RNA flow cytometry. When MLC effector cells derived from a G14D-CCV–immunized fish were preincubated with CC41 mAb, killing of G14D-CCV targets was reduced by ∼40%, suggesting that at least some LITRs have a role in target cell recognition and/or cytotoxicity. The availability of a LITR-specific mAb has allowed, to our knowledge for the first time, functional characterization of LITRs in an autologous system. In addition, the identification of an LITR subset as a cytotoxic cell marker will allow for more effective monitoring of catfish immune responses to pathogens.

show abstract

Diversity of the FcR- and KIR-related genes in an amphibian Xenopus

Cited by 12 publications

References 61 publications

Tumorigenesis and anti-tumor immune responses in Xenopus

Tumorigenesis and anti-tumor immune responses in Xenopus

Immunoreceptor tyrosine‐based inhibition motifs: a quest in the past and future

A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus

Contact Info

Product

Resources

About