Diversity of the Swine Leukocyte Antigen Class I and II in Commercial Pig Populations

Techakriengkrai, Navapon; Nedumpun, Teerawut; Golde, William T.; Suradhat, Sanipa

doi:10.3389/fvets.2021.637682

Cited by 11 publications

(12 citation statements)

References 40 publications

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“…For SLA class II, the most frequent genotype was GT-0.5 with a frequency of 32.05% and GT-0.6 with a frequency of 27.75%, respectively. Comparing these results with published data from Techakriengkrai and co-workers, we can speculate that the unknown haplotype SLA-1*Blank/SLA-3*04XX/SLA-2*11XX might be the same as Lr-55.0mod found in the present study ( 33 ). As this haplotype occurred in only one animal, this needs to be further investigated.…”

Section: Discussionsupporting

confidence: 89%

“…However, among the studied cohorts of Göttingen Minipigs, a couple of potential private haplotypes were found that may be specific only to these Minipigs. Commercial pigs exhibit a high SLA diversity being resembled in 91 SLA class I and 47 SLA class II haplotypes that have been so far identified in various pig lines ( 27 – 29 , 32 , 33 , 36 , 37 ). This assumption is supported by the restricted occurrence of the SLA class I haplotype Lr-Hp 10.0 and the SLA class II haplotypes Lr-Hp 0.3, Lr-Hp 0.17 and Lr-Hp 0.31mod being exclusively found in Göttingen Minipigs.…”

Section: Discussionmentioning

confidence: 99%

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Comparative analysis of swine leukocyte antigen gene diversity in Göttingen Minipigs

Hammer,

Duckova,

Gociman

et al. 2024

Front. Immunol.

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Worldwide, pigs represent economically important farm animals, also representing a preferred preclinical large animal model for biomedical studies. The need for swine leukocyte antigen (SLA) typing is increasing with the expanded use of pigs in translational research, infection studies, and for veterinary vaccine design. Göttingen Minipigs (GMP) attract increasing attention as valuable model for pharmacological studies and transplantation research. This study represents a first-time assessment of the SLA gene diversity in Göttingen Minipigs in combination with a comparative metadata analysis with commercial pig lines. As Göttingen Minipigs could harbor private as well as potential novel SLA allele combinations, future research projects would benefit from the characterization of their SLA background. In 209 Göttingen Minipigs, SLA class I (SLA-1, SLA-2, SLA-3) and class II (DRB1, DQB1, DQA) genes were characterized by PCR-based low-resolution (Lr) haplotyping. Criteria and nomenclature used for SLA haplotyping were proposed by the ISAG/IUIS-VIC SLA Nomenclature Committee. Haplotypes were assigned based on the comparison with already known breed or farm-specific allele group combinations. In total, 14 SLA class I and five SLA class II haplotypes were identified in the studied cohort, to manifest in 26 SLA class I but only seven SLA class II genotypes. The most common SLA class I haplotypes Lr-24.0 (SLA-1*15XX or Blank-SLA-3*04:04-SLA-2*06:01~02) and Lr-GMP-3.0 (SLA-1*16:02-SLA-3*03:04-SLA-2*17:01) occurred at frequencies of 23.44 and 18.66%, respectively. For SLA class II, the most prevalent haplotypes Lr-0.21 (DRB1*01XX-DQB1*05XX-DQA*04XX) and Lr-0.03 (DRB1*03:02-DQB1*03:01-DQA*01XX) occurred at frequencies of 38.28 and 30.38%. The comparative metadata analysis revealed that Göttingen Minipigs only share six SLA class I and two SLA class II haplotypes with commercial pig lines. More importantly, despite the limited number of SLA class I haplotypes, the high genotype diversity being observed necessitates pre-experimental SLA background assessment of Göttingen Minipigs in regenerative medicine, allo-transplantation, and xenograft research.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Comparative analysis of swine leukocyte antigen gene diversity in Göttingen Minipigs

Hammer,

Duckova,

Gociman

et al. 2024

Front. Immunol.

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“…SLA haplotype identi cation can be utilized alongside immunohematological tests, evaluating the biomodels' histocompatibility. The porcine leukocyte antigen (SLA) has a similar structure to human leukocyte antigen (HLA), which is a signi cant variable for determining the longevity of an allograft (55,56). These speci c haplotypes have a high degree of polymorphism and immunoreactivity, being essential for determining compatibility between donor and recipient.…”

Section: Surgical Protocolmentioning

confidence: 99%

“…Within this scale, the biopsy corresponds to Grade 0 rejection, where in ammatory in ltrate is rarely observed, and the epithelium is not involved (50). Thus, even with blood and/or histological incompatibility, and in the absence of immunosuppression, no hyperacute rejection was evident, which should usually occur from minutes to hours after transplantation (55).…”

Section: Surgical Protocolmentioning

confidence: 99%

An innovative Ear Transplantation for Vascularized Composite Allotransplantation Research in Porcine Model

Holguín-Ruíz,

Rodríguez-Galviz,

Galvao

et al. 2024

Preprint

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Vascularized composite allotransplantation (VCA) represents a clinical challenge for transplant therapy, as it involves different tissues with unique immunogenicity. Even when receiving immunosuppressive therapy, they are more vulnerable to severe hypoxia, microvascular damage, and ultimately the rejection or chronic graft dysfunction after transplantation. This study aimed to develop a surgical protocol for VCA of the ear in a porcine biomodel in the absence of immunosuppression, maintaining the in vitro co-culture of the allograft and assessing their relationship with allograft survival. We employed five crossbred pigs and three outbred mini pig biomodels (Sus scrofa), as donors and recipients, to perform four VCAs. Blood samples were taken from each biomodel for crossmatch testing and SLA haplotype identification. Bone marrow samples were taken from each recipient for subsequent co-culture. Temperature variations between culture and co-culture period were assessed. Histological analysis was performed on the allografts that survived beyond eight days, using hematoxylin and eosin staining. A surgical protocol for Vascularized Composite Allograft (VCA) ear transplantation in a porcine biomodel was developed, including the skin. The presence of SLA-DRB1*01:02 and SLA-DRB1*06:01 haplotypes in the recipient and donor, respectively, showed concordance with positive crossmatch tests. No histological signs of hyperacute rejection were found ten days after transplantation. The results obtained from this protocol can provide valuable recommendations for translational applications in face transplantation, extremity transplantation, and regenerative medicine.

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“…Regarding veterinary studies, a study has explored the MHC in different species. Allele frequency and diversity in pigs has been analysed in wild and production species which are of interest, due to their biological characteristics, their use in biomedical studies, transplants, regenerative medicine, and vaccine design [53,55,56]. Hammer et al (2021), for example, used low-resolution (Lr) SLA haplotyping for characterising European SLA-I (SLA-1, SLA-2, and SLA-3) and SLA-II genes (DRB1, DQB1, DQA) in 549 pigs representing nine commercial lines from various production systems; they identified 50 class I haplotypes and 37 class II ones.…”

Section: Genetic Variability and Immune Responsementioning

confidence: 99%

Nanovaccines against Animal Pathogens: The Latest Findings

et al. 2021

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Nowadays, safe and efficacious vaccines represent powerful and cost-effective tools for global health and economic growth. In the veterinary field, these are undoubtedly key tools for improving productivity and fighting zoonoses. However, cases of persistent infections, rapidly evolving pathogens having high variability or emerging/re-emerging pathogens for which no effective vaccines have been developed point out the continuing need for new vaccine alternatives to control outbreaks. Most licensed vaccines have been successfully used for many years now; however, they have intrinsic limitations, such as variable efficacy, adverse effects, and some shortcomings. More effective adjuvants and novel delivery systems may foster real vaccine effectiveness and timely implementation. Emerging vaccine technologies involving nanoparticles such as self-assembling proteins, virus-like particles, liposomes, virosomes, and polymeric nanoparticles offer novel, safe, and high-potential approaches to address many vaccine development-related challenges. Nanotechnology is accelerating the evolution of vaccines because nanomaterials having encapsulation ability and very advantageous properties due to their size and surface area serve as effective vehicles for antigen delivery and immunostimulatory agents. This review discusses the requirements for an effective, broad-coverage-elicited immune response, the main nanoplatforms for producing it, and the latest nanovaccine applications for fighting animal pathogens.

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Diversity of the Swine Leukocyte Antigen Class I and II in Commercial Pig Populations

Cited by 11 publications

References 40 publications

Comparative analysis of swine leukocyte antigen gene diversity in Göttingen Minipigs

Comparative analysis of swine leukocyte antigen gene diversity in Göttingen Minipigs

An innovative Ear Transplantation for Vascularized Composite Allotransplantation Research in Porcine Model

Nanovaccines against Animal Pathogens: The Latest Findings

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