Diversity, rearrangement, and expression of murine T cell gamma genes

Garman, Richard D.; Doherty, Philip J.; Raulet, David H.

doi:10.1016/0092-8674(86)90787-7

Cited by 392 publications

(256 citation statements)

References 36 publications

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“…The Vy2, Vy4, and Vy1 .2 probes were those described in Garman et al . (10). The Cy4 probe is a 1.6 kb Eco RI-Bam HI genomic fragment that includes the first exon of Cy4 and -700 by of DNA in the Jy4-Cy4 intron .…”

Section: Resultsmentioning

confidence: 99%

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Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

Korman

Marusić-Galesić

Spencer

et al. 1988

The Journal of Experimental Medicine

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Most T lymphocytes express a clonally distributed cell surface antigen receptor composed of disulfide-linked a and (3 subunits, noncovalently associated with several subunits called CD3 proteins . T cells that express the a/(3 form of the T cell receptor (TCR) recognize antigens in the context of cell surface proteins encoded by class I and class II genes of the major histocompatibility complex (MHC). A small distinct subset of peripheral T lymphocytes (

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Section: Resultsmentioning

confidence: 99%

“…4 A) (9)(10)(11)(12)(13) . Therefore, the potential V-region diversity of the y chain is limited compared with that of the immunoglobulin heavy or light chains, and the a and ß subunits of the a/ß TCR .…”

mentioning

confidence: 99%

Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

Korman

Marusić-Galesić

Spencer

et al. 1988

The Journal of Experimental Medicine

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“…Results and Discussion Six TCR-7/6-expressing hybridomas (1C6.F11 and 1H11 .B9 of C3H/HeN origin and DN12 .1, DN7.1, DN7 .3, and DN2 .3 of C57BL/6 origin) were used in this study. Earlier studies (2,6,12,13) have indicated that all these hybridomas express glycosylated y chains that are not derived from Vy-Cy4 transcripts since the antiserum used to detect these y chains does not react with Cy4 chains (nomenclature according to Garman et al, [10]). Furthermore, since the Cy3 locus is a pseudogene (8) and, at least for the C57BL/6 mice, V71 .2-C"y2 transcripts would result in nonglycosylated y chains (19), these hybridomas most likely express y chains derived from Vy-CyI transcripts.…”

Section: Methodsmentioning

confidence: 99%

“…The y chain gene family is known to encode four C regions (1 pseudogene) and seven V regions which can give rise to at least five y chain mRNA transcripts (8)(9)(10)(11)(12)(13) . It is unknown if all these transcripts give rise to cell surface-expressed proteins.…”

mentioning

confidence: 99%

T cell receptor gamma/delta chain diversity.

Koning

Kruisbeek

Maloy

et al. 1988

The Journal of Experimental Medicine

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The TCR-gamma and -delta chains of six murine hybridomas were compared by one-dimensional SDS-PAGE and two-dimensional NEPHGE/SDS-PAGE analysis. This allowed the identification of three distinct gamma chains (gamma a, gamma b, and gamma c) and three distinct delta chains (delta a, delta b, and delta c). Four gamma/delta chain combinations (gamma a delta a, gamma b delta b, gamma b delta c, and gamma c delta a) were observed. These results indicate that multiple forms of the delta chain are expressed and suggest that the delta chains are encoded for by an Ig-like rearranging gene. This delta chain polymorphism significantly enhances the potential diversity of TCR-gamma/delta, which may be of importance for a better understanding of the putative ligand(s) recognized by this receptor.

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“…iIELs were isolated from the small intestine according to published procedures (14). Spleens and thymuses were mechanically disrupted, followed for the splenocytes by lysis of the erythrocytes with 0.17 M NH 4 Cl.…”

Section: Methodsmentioning

confidence: 99%

DifferentialLy49eExpression Pathways in Resting versus TCR-Activated Intraepithelial γδ T Cells

Broeck

Ammel

Delforche

et al. 2013

The Journal of Immunology

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The Ly49 NK receptor family in mice is composed of several members that recognize MHC class I (MHC-I) or MHC-I–related molecules. We and others have shown before that Ly49E is a unique member, with a different expression pattern on NK cells and being triggered by the non–MHC-I–related protein urokinase plasminogen activator. Among the entire Ly49 receptor family, Ly49E is the only Ly49 member expressed by epidermal-localized γδ T cells and their fetal thymic TCRγδ precursors, and it is the most abundantly expressed member on intestinal intraepithelial γδ T cell lymphocytes. In this study, we provide mechanistic insights into the regulation of Ly49e expression in γδ T cells. First, we demonstrate that TCR-mediated activation of intraepithelial γδ T cells significantly increases Ly49E expression. This results from de novo Ly49E expression and is highly selective, because no other Ly49 family members are induced. TCR-mediated Ly49E induction is a conserved feature of skin- and gut-residing intraepithelial-localized γδ T cell subsets, whereas it is not observed in spleen γδ T cells. By investigating Ly49e promoter activities and lymphotoxin (LT) αβ dependency in resting versus TCR-activated intraepithelial γδ T cells, we reveal two separate regulatory pathways for Ly49E expression, as follows: a LTαβ-dependent pathway leading to basal Ly49E expression in resting cells that is induced by Pro2-mediated Ly49e transcription, and a LTαβ-independent pathway leading to elevated, Pro3-driven Ly49E expression in TCR-stimulated cells.

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Diversity, rearrangement, and expression of murine T cell gamma genes

Cited by 392 publications

References 36 publications

Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

T cell receptor gamma/delta chain diversity.

DifferentialLy49eExpression Pathways in Resting versus TCR-Activated Intraepithelial γδ T Cells

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