Diversity resistance to Puccinia striiformis f. sp Tritici in rye chromosome arm 1RS expressed in wheat

Yang, Meiyin; Ren, Tao; Yan, Bin; Li, Z.; Ren, Zhenglong

doi:10.4238/2014.october.27.20

Cited by 16 publications

(18 citation statements)

References 34 publications

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“…These new T1RS.1BL translocation lines showed diversity-resistant patterns to rust stripe and powdery mildew. However, few new translocation lines showed both high resistance to these two diseases and could be used in the wheat breeding program (Ko et al, 2002; Yang et al, 2014; Li et al, 2016b; Qi et al, 2016). …”

Section: Discussionmentioning

confidence: 99%

“…Since the 1990s, due to its single origin, the 1RS chromosome arm derived from Petkus rye has not provided protection against the prevalence of virulent pathogens (Shi et al, 2001; Ren et al, 2009). So far, only a few other sources of 1RS were transferred into wheat (Ko et al, 2002; Mater et al, 2004; Ren et al, 2009, 2012; Molnár-Láng et al, 2010; Yang et al, 2014; Li et al, 2016a,b; Qi et al, 2016), and not every new translocation line could be used in wheat breeding programs (Ren et al, 2012). For more efficient use of the T1RS.1BL translocation in wheat breeding, Ren et al (2012) suggested introduce a large amount of new genetic variation from many different rye sources into wheat.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Cytogenetic Characterization of Novel Wheat-rye T1RS.1BL Translocation Lines with High Resistance to Diseases and Great Agronomic Traits

Ren

Tang

et al. 2017

Front. Plant Sci.

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Rye has been used worldwide as a source for the genetic improvement of wheat. In this study, two stable wheat-rye primary T1RS.1BL translocation lines were selected from the progeny of the crossing of the wheat cultivar Mianyang11-1 and a Chinese local rye variety, Weining. These two novel translocation lines were identified by molecular cytogenetic analysis. PCR results, multi-color fluorescence in situ hybridization (MC-FISH), and acid polyacrylamide gel electrophoresis (A-PAGE) indicated that both new translocation lines harbor a pair of T1RS.1BL translocation chromosomes, and have been named RT828-10 and RT828-11, respectively. The cytogenetic results also indicated that the pSc119.2 signals of 5AL were absent in both lines along with the pSc119.2 signals of 4AL of RT828-11. When inoculated with different stripe rust and powdery mildew isolates, both lines expressed high resistance to Puccinia striiformis f. sp. tritici and Blumeria graminis f. sp. tritici pathotypes, which are prevalent in China and are virulent on Yr9 and Pm8. The line RT828-11 also exhibited excellent agronomic traits in the field. The present study indicates that this rye variety may carry untapped variations that could potentially be used for wheat improvement.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Cytogenetic Characterization of Novel Wheat-rye T1RS.1BL Translocation Lines with High Resistance to Diseases and Great Agronomic Traits

Ren

Tang

et al. 2017

Front. Plant Sci.

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“…Therefore, the sources of the 1RS chromosome arm were limited, and there are very few genetic variations in this 1RS arm [ 14 , 15 ]. Thus far, only a few other sources of 1RS have been introduced into the wheat genome [ 5 , 15 , 31 , 19 , 47 , 48 , 49 , 50 ]. Of these translocation lines, however, far fewer have reached significant commercial production [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…AF245032). PrCEN-2 was used to produce a rye-specific centromeric sequence and to analyze the structure of the centromere of the 1RS.1BLchromosomes [ 31 ]. 8 SSR markers with clear bands which specific for 1RS arm were used to test the genetic diversity of 1RS chromosome arm of 4 different wheat lines ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Molecular Cytogenetic Characterization of New Wheat-Rye 1R(1B) Substitution and Translocation Lines from a Chinese Secale cereal L. Aigan with Resistance to Stripe Rust

Ren

Tan

et al. 2016

PLoS ONE

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Secale cereale L. has been used worldwide as a source of genes for agronomic and resistance improvement. In this study, a stable wheat-rye substitution line and 3 primary 1RS.1BL translocation lines were selected from the progeny of the crossing of the Chinese local rye Aigan variety and wheat cultivar Mianyang11. The substitution and translocation lines were identified by molecular cytogenetic analysis. PCR results, fluorescence in situ hybridization and acid polyacrylamide gel electrophoresis indicated that there were a pair of 1R chromosomes in the substitution line which have been named RS1200-3, and a pair of 1RS.1BL translocation chromosomes in the other 3 translocation lines, which have been named RT1163-4, RT1217-1, and RT1249. When inoculated with stripe rust isolates, these 4 lines expressed high resistance to several Puccinia striiformis f. sp Tritici pathotypes that are virulent on Yr9. Moreover, the different response pattern of resistance among them suggested that the diversity of resistance genes for wheat stripe rust exists in the rye. These 4 lines also showed better agronomic performances than their wheat parent. The GS indices also showed the genetic diversity of the 1RS which derived from same rye variety. The present study indicates that rye cultivars may carry untapped variations that could potentially be used for wheat improvement.

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“…Regarding rye genepool as resistance source for wheat improvement, Wang et al (2009) 59 transferred 1RS arm from the rye cultivar German White into the chinese wheat cultivar Xiaoyan 6, and www.nature.com/scientificreports www.nature.com/scientificreports/ produced a T2BL.1RS wheat-rye chromosome translocation line resistant to stripe rust and powdery mildew. Using Chinese wheat and rye cultivars, various T1BL.1RS translocation lines resistant to stripe rust 60 or both stripe rust and powdery mildew [61][62][63] were also developed. In case of wild relatives of rye, only two papers reporting on the transfer of stripe rust resistance from S. africanum have been published 64,65 , where the resistance genes were mapped on the chromosome arms 1RS 64 and 2RL 65 .…”

Section: Effects On Morphological and Quality Traits The Novel Genetmentioning

confidence: 99%

1RS arm of Secale cereanum ‘Kriszta’ confers resistance to stripe rust, improved yield components and high arabinoxylan content in wheat

Szakács

Szőke-Pázsi

Kalapos

et al. 2020

Sci Rep

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Wheat-rye T1BL.1RS translocation is widespread worldwide as the genes on 1RS arm have positive effect on stress resistance, grain yield and adaptation ability of wheat. Nowadays, the T1BL.1RS wheat cultivars have become susceptible to rust diseases because of the monophyletic ('Petkus') origin of 1RS. Here we report and discuss the production and detailed investigation of a new T1BL.1RS translocation line carrying 1RS with widened genetic base originating from Secale cereanum. Line '179' exhibited improved spike morphology traits, resistance against stripe rust and leaf rust, as well as higher tillering capacity, fertility and dietary fiber (arabynoxylan) content than the parental wheat genotype. Comparative analyses based on molecular cytogenetic methods and molecular (SSR and DArTseq) makers indicate that the 1RS arm of line '179' is a recombinant of S. cereale and S. strictum homologues, and approximately 16% of its loci were different from that of 'Petkus' origin. 162 (69.5%) 1RS-specific markers were associated with genes, including 10 markers with putative disease resistance functions and LRR domains found on the subtelomeric or pericentromeric regions of 1RS. Line '179' will facilitate the map-based cloning of the resistance genes, and it can contribute to healthy eating and a more cost-efficient wheat production. Interspecific hybridization is one of the most promising way to improve the genetic diversity of bread wheat 1. The most widely known wheat-alien introgression has been the spontaneous translocation T1BL.1RS that is traced back to the cross of wheat 'Criewener 104' × rye 'Petkus Roggen' made in Germany between 1920 and 1930 2. Its effect on morphology and baking quality of bread wheat has been investigated in numerous studies. The positive effect of this translocation on grain yield of hexaploid wheat 3,4 and durum wheat 5,6 is commonly accepted, though in some cases no such effect was shown 7,8. Association between the presence of 1RS and increase in root biomass, water use efficiency as well as harvest index was also found in glasshouse and field experiments 9,10. 1RS arm also contains genes (Pm8, Lr26, Sr31 and Yr9) providing resistance against powdery mildew (Erysiphe graminis f. sp. tritici), leaf rust (Puccinia triticina), stem rust (Puccinia graminis f. sp. tritici), and stripe or yellow rust (Puccinia striiformis f. sp. tritici), respectively 11,12 , a reason that led to the widespread utilization of the T1BL.1RS translocation in wheat improvement. However, the resistance genes Lr26, Yr9 and Pm8 are no longer effective against new virulent biotypes of the pathogens in Europe 13. Virulence to the Sr31 resistance gene has also been reported from Uganda 14 , and from Kenya, Ethiopia, Sudan, and Iran 15. Spread of the Sr31-virulent pathotype in countries where wheats carrying the T1BL.1RS chromosome are cultivated can cause serious problems for agriculture as the genetic vulnerability of T1BL.1RS cultivars is the consequence of the lack of allelic variation on the 'Petkus'-derived 1RS arm 4. ...

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Diversity resistance to Puccinia striiformis f. sp Tritici in rye chromosome arm 1RS expressed in wheat

Cited by 16 publications

References 34 publications

Molecular Cytogenetic Characterization of Novel Wheat-rye T1RS.1BL Translocation Lines with High Resistance to Diseases and Great Agronomic Traits

Molecular Cytogenetic Characterization of Novel Wheat-rye T1RS.1BL Translocation Lines with High Resistance to Diseases and Great Agronomic Traits

Molecular Cytogenetic Characterization of New Wheat-Rye 1R(1B) Substitution and Translocation Lines from a Chinese Secale cereal L. Aigan with Resistance to Stripe Rust

1RS arm of Secale cereanum ‘Kriszta’ confers resistance to stripe rust, improved yield components and high arabinoxylan content in wheat

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