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Халдеева, Е. В.; Medyantseva, É. P.; Imanaeva, N. A.; Budnikov, H. C.

doi:10.1023/a:1021480500400

Journal of Analytical Chemistry

2002

DOI: 10.1023/a:1021480500400

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Е. В. Халдеева

É. P. Medyantseva

N. A. Imanaeva

et al.

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Cited by 10 publications

(5 citation statements)

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“…Today, kits for the Biacore sensor to test for sulfamethazine, sulfadiazine, streptomycin, and chloramphenicol are available. Besides SPR, a number of other biosensors for the rapid detection of antibiotics have been described. − The system Parallux, commercially available from Idexx Laboratories (Westbrook, MA), is a rapid test working with competitive solid-phase fluorescence immunoassays in four channels, making it possible to use four antibodies simultaneously within one test. , Although most of these sensors offer sufficient performance, most of them allow only the use of one antibody or receptor protein. Two systems enable the use of four antibodies in parallel, but the number of analytes still remains very limited.…”

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“…Besides SPR, a number of other biosensors for the rapid detection of antibiotics have been described. [21][22][23][24][25] The system Parallux, commercially available from Idexx Laboratories (Westbrook, MA), is a rapid test working with competitive solid-phase fluorescence immunoassays in four channels, making it possible to use four antibodies simultaneously within one test. 26,27 Although most of these sensors offer sufficient performance, most of them allow only the use of one antibody or receptor protein.…”

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Automated Microarray System for the Simultaneous Detection of Antibiotics in Milk

et al. 2003

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A parallel affinity sensor array (PASA) for the rapid automated analysis of 10 antibiotics in milk is presented, using multianalyte immunoassays with an indirect competitive ELISA format. Microscope glass slides modified with (3-glycidyloxypropyl)trimethoxysilane were used for the preparation of hapten microarrays. Protein conjugates of the haptens were immobilized as spots on disposable chips, which were processed in a flow cell. Monoclonal antibodies against penicillin G, cloxacillin, cephapirin, sulfadiazine, sulfamethazine, streptomycin, gentamicin, neomycin, erythromycin, and tylosin allowed the simultaneous detection of the respective analytes. Antibody binding was detected by a second antibody labeled with horseradish peroxidase generating enhanced chemiluminescence, which was recorded with a sensitive CCD camera. All liquid handling and sample processing was fully automated, and one analysis was carried out in milk within less than 5 min. The detection limits ranged from 0.12 (cephapirin) to 32 microg/L (neomycin). Penicillin G could be detected at the maximum residue limit (MRL); the detection limits for all other analytes were far below the respective MRLs. The PASA system proved to be the first immunochemical biosensor platform having the potential to test for numerous antibiotics in parallel, such being of considerable interest for the control of milk in the dairy industry.

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Automated Microarray System for the Simultaneous Detection of Antibiotics in Milk

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“…Microbiological [2], spectroscopic [3][4][5], and chromatographic [6,7] techniques have been used for determining aminoglycoside antibiotics, whereas electrochemical techniques have been rarely used [8].…”

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Rapid Potentiometric Determination of Aminoglycoside Antibiotics in Drug Dosage Forms and Biological Fluids Using Ion-Selective Electrodes

2005

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Aminoglycosides are broad-spectrum antibiotics; they exhibit specific ototoxicity and nephrotoxicity and can cause respiratory depression up to the development of muscular blockade. Therefore, therapeutic drug monitoring is required in the majority of cases [1].Practical demands for monitoring the main component concentration in pharmaceutical dosage forms and the necessity of determining aminoglycosides in biological fluids stimulate research into the development of rapid analytical methods. Microbiological [2], spectroscopic [3][4][5], and chromatographic [6,7] techniques have been used for determining aminoglycoside antibiotics, whereas electrochemical techniques have been rarely used [8].This work is devoted to a study of the electroanalytical properties of membranes sensitive to aminoglycoside antibiotics and the development of procedures for the rapid potentiometric determination of gentamycin and kanamycin in biological fluids and pharmaceuticals using ion-selective electrodes (ISEs). EXPERIMENTAL Gentamycin (Gen) sulfate (4% solution in ampoules) and kanamycin (Kan) sulfate (in vials) were used. Aqueous stock solutions (1.0 × 10 -2 M) were prepared using accurately weighed portions of the preparations. Solutions with 1.0 × 10 -2 -1.0 × 10 -5 M concentrations were prepared by successive dilution. Table 1 summarizes the names, formulas, and characteristics of the substances used in this study. Sodium tetraphenylborate (TPB) and the Acid Chrome Black Special (ABS) azo dye were used as precipitant anions for preparing the active components of membranes.The gentamycin-tetraphenylborate ionophore was prepared by mixing sodium tetraphenylborate and gentamycin sulfate solutions in equimolar amounts ( c Gen = c TPB = 1.0 × 10 -2 M; V Gen = V TPB = 20.0 mL) at room temperature. The Gen-ABS and Kan-ABS ion pairs were prepared by mixing solutions of the antibiotics ( c = 1.0 × 10 -2 ) with an alcoholic solution of ABS ( c = 2%) at the ratios Gen : ABS = 1 : 5 and Kan : ABS = 1 : 4. The precipitates were allowed to stand for a day, washed with distilled water by decantation, filtered off, and dried in a drying oven at 60-70 ° C.We studied liquid electrodes with homemade plasticized PVC membranes ( c ionophore = 0.01 mol/(kg dibutyl phthalate (DBP)); the PVC-DBP weight ratio was 1 : 3). The internal solution contained a 1 : 1 mixture of a 1.0 × 10 -3 M antibiotic solution and a 1.0 × 10 -3 M Na 2 SO 4 solution. The emf of the circuit Ag, AgCl | internal solution | membrane | test solution | KCl sat | AgCl, Ag was measured to within ± 0.001 V using an I-130 potentiometer; an EMV-1MZ silver-silver chloride electrode served as a reference electrode.The thermoanalytical study of the ionophores was performed on an OD-103 derivatograph, which allowed us to perform programmed heating from room temperature to 1000 ° C in air. Temperature was measured with a Pt/Pt-Rh thermocouple; calcined aluminum oxide served as a reference substance.Abstract -Ion-selective electrodes with plasticized membranes based on ion pairs of gentamyci...

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“…However, the relatively low therapeutic ratios of these drugs makes their successful administration possible only if the concentration of the active compound in the blood is thoroughly controlled. Only a few papers have been devoted to the development of electrochemical methods for the determination of antibiotics of the aminoglycoside group (see, e.g., [6]). Our analysis of the published data showed that the quantitative determination of aminoglycoside antibiotics is usually performed using microbiological [3], spectrophotometric [4], and chromatographic [5] techniques.…”

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Ionometric determination of Gentamicin and Kanamycin in biological fluids and ready-to-use medicinal forms

Kulapina¹,

Baraguzina²,

Kulapina³

2004

Pharm Chem J

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Aminoglycoside antibiotics occupy leading positions in the treatment of serious infectious-inflammatory disorders. However, the relatively low therapeutic ratios of these drugs makes their successful administration possible only if the concentration of the active compound in the blood is thoroughly controlled. Slow elimination of aminoglycosides may result in oto-and nephrotoxicity manifestations [1,2].The practical need for reliable and convenient methods for monitoring the level of antibiotics in the blood stimulates investigations devoted to the development of such methods for determining aminoglycosides. Our analysis of the published data showed that the quantitative determination of aminoglycoside antibiotics is usually performed using microbiological [3], spectrophotometric [4], and chromatographic [5] techniques. Only a few papers have been devoted to the development of electrochemical methods for the determination of antibiotics of the aminoglycoside group (see, e.g., [6]).The aim of this study was to develop an ionometric technique for determining gentamicin and kanamycin in various medicinal forms and biological fluids. EXPERIMENTAL PARTThe experiments were performed with commercial gentamicin sulfate (4% injection solution in ampules) and kanamycin sulfate (lyophilized powder in flasks). The initial stock (1´10 -2 M) solutions were prepared using accurately weighed amounts of the initial drugs, and the less concentrated solutions (from 1´10 -3 to 1´10 -5 M) were obtained by serial dilutions. The names, structural formulas, and molar weights of the studied and auxiliary compounds are given in Table 1.The electrode-active compound (EAC) was gentamicin tetraphenylborate (Gen-TPB) obtained by mixing equimolar amounts of sodium tetraphenylborate (Na-TPB) and gentamicin sulfate (C TPB =10 -2 M, V = 20 ml; C Gen =10 -2 M, V = 20 ml) at room temperature. The mixture was allowed to stand for three days for precipitation, after which the precipitate was washed by decantation with distilled water and dried in a thermal box at 60°C.The electrochemical measurements were performed using ion-selective electrodes (ISEs) based on Gen-TPB ion associates with poly(vinyl chloride) PVC membranes plasticized by dibutylphthalate (DBP) in the ratio PVC : DBP =1 : 3 (C EAC = 0.01 mole/kg DBP). The liquid-filled film electrodes had the form of PVC tubes with membrane disks of the same diameter glued to thoroughly lapped edge faces. The glue was prepared by dissolving 0.5 g PVC in 5 ml of tetrahydrofuran or cyclohexane. After drying of the glue, the electrode tube was filled with the standard solution comprising a mixture of the corresponding antibiotic solution (C =10 -3 M) and sodium sulfate (C =10 -3 M) in a 1 :1 ratio. Prior to measurements, the ISE was conditioned in a 10 -3 M solution of the corresponding antibiotic.The potentiometric procedure was performed in a cell with a silver chloride reference electrode (EVL-1M3) using an I-130 potentiometer; the error of emf measurements was ± 1 mV. The pH measurements were perform...

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Untitled

Cited by 10 publications

References 10 publications

Automated Microarray System for the Simultaneous Detection of Antibiotics in Milk

Automated Microarray System for the Simultaneous Detection of Antibiotics in Milk

Rapid Potentiometric Determination of Aminoglycoside Antibiotics in Drug Dosage Forms and Biological Fluids Using Ion-Selective Electrodes

Ionometric determination of Gentamicin and Kanamycin in biological fluids and ready-to-use medicinal forms

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