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Bach, Inga C.; Olesen, Annette; Simon, Philipp W.

doi:10.1023/a:1020314802236

Cited by 33 publications

(3 citation statements)

References 30 publications

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“…As in other angiosperm mitochondrial genomes, most of the known genes encode for proteins of the electron transport chain, with 9 subunits of complex I ( nad1 2 3 4 4 L 5 6 7 and 9 ), one subunit of complex III ( cob ), three subunits of complex IV ( cox1 2 and 3 ), five subunits of complex V ( atp1 4 6 8 and 9 ) and four genes involved in the biogenesis of cytochrome c ( ccmB ccmC ccmFc and ccmFn ). Truncated copies of atp1 and atp9 were detected, confirming observations previously reported by [29,30]. In addition, two conserved ORFs, coding for a maturase ( matR ) mapped within the nad1 intron and another ORF named mttB coding for a transporter protein, were detected.…”

Section: Resultssupporting

confidence: 89%

De novo assembly of the carrot mitochondrial genome using next generation sequencing of whole genomic DNA provides first evidence of DNA transfer into an angiosperm plastid genome

et al. 2012

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BackgroundSequence analysis of organelle genomes has revealed important aspects of plant cell evolution. The scope of this study was to develop an approach for de novo assembly of the carrot mitochondrial genome using next generation sequence data from total genomic DNA.ResultsSequencing data from a carrot 454 whole genome library were used to develop a de novo assembly of the mitochondrial genome. Development of a new bioinformatic tool allowed visualizing contig connections and elucidation of the de novo assembly. Southern hybridization demonstrated recombination across two large repeats. Genome annotation allowed identification of 44 protein coding genes, three rRNA and 17 tRNA. Identification of the plastid genome sequence allowed organelle genome comparison. Mitochondrial intergenic sequence analysis allowed detection of a fragment of DNA specific to the carrot plastid genome. PCR amplification and sequence analysis across different Apiaceae species revealed consistent conservation of this fragment in the mitochondrial genomes and an insertion in Daucus plastid genomes, giving evidence of a mitochondrial to plastid transfer of DNA. Sequence similarity with a retrotransposon element suggests a possibility that a transposon-like event transferred this sequence into the plastid genome.ConclusionsThis study confirmed that whole genome sequencing is a practical approach for de novo assembly of higher plant mitochondrial genomes. In addition, a new aspect of intercompartmental genome interaction was reported providing the first evidence for DNA transfer into an angiosperm plastid genome. The approach used here could be used more broadly to sequence and assemble mitochondrial genomes of diverse species. This information will allow us to better understand intercompartmental interactions and cell evolution.

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Section: Resultssupporting

confidence: 89%

De novo assembly of the carrot mitochondrial genome using next generation sequencing of whole genomic DNA provides first evidence of DNA transfer into an angiosperm plastid genome

et al. 2012

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“…Molecular genetic analysis was then carried out to differentiate plants according to the "petaloid" and normal types of cytoplasm. The polymerase chain reaction was performed according to the protocol by BACH et al [2002].…”

Section: Methodsmentioning

confidence: 99%

Journal of Water and Land Development

Voronina,

Vishnyakova,

Monakhos

et al. 2022

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Using doubled haploid technologies inbreeding can significantly reduce the time to obtain homozygous parental lines required for the production of F1-hybrid of vegetable crops. This study aims to investigate the influence of factors on the efficiency of carrot embryogenesis in isolated microspore culture to optimise the elements of protocol for producing doubled haploids. Microspores were isolated from inflorescences of 21 genotypes and incubated in NLN13 medium supplemented with 0.1 mg•dm -3 2,4-dichlorophenoxyacetic acids, 0.1 mg•dm -3 1-naphthyl acetic acids, 130 g•dm -3 sucrose, and 400 mg•dm -3 casein hydrolysate and its modifications. Embryoids and their groups were formed after 2-6 months, in some cases after 12 months of cultivation. Depending on the variant, the embryogenesis efficiency averaged from 0 to 4.9 embryoids or groups of embryoids per Petri dish (10 cm 3 ). Embryoids within the group were formed from different microspores. No significant effects of inflorescence position on the plant (branching order), sucrose, and casein hydrolysate concentration in the medium were observed. Significant advantages (p ≥ 0.05) for some genotypes were shown: 1) microspore suspension density 4•10 4 cells•cm -3 (5.0 embryoids per Petri dish were formed at a microspore suspension density of 4•10 4 cells•cm -3 , 0.0 embryoids per Petri dish at a density of 8•10 4 cells•cm -3 ); 2) cultivating microspores of tetrad and early mononuclear stage (4.9 ±3.1 embryoids per Petri dish were obtained by culturing tetrads and early mononuclear microspores, while 0.6 ±0.7 embryoids per Petri dish were obtained by culturing of later developmental stages); 3) high-temperature treatment duration of five days (4.9 ±2.1 embryoids per Petri dish were obtained after five days of high-temperature treatment, 2.7 ±2.6 embryoids per Petri dish formed after two days of high-temperature treatment; 9.8 ±4.7, 10.1 ±6.1, 0.0 ±0.0 embryoids per Petri dish formed after two, five and eight days of high-temperature treatment respectively); 4) adding colchicine 0.5 mg•dm -3 to the nutrient medium for two days of high-temperature treatment, followed by medium replacement (3.3 ±2.6 embryoids per Petri dish were obtained by using a nutrient medium with colchicine, while 1.7 ±1.5 embryoids per Petri dish were obtained by culturing in the reference variant).

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“…Male sterility plays an important role not only in genetic breeding but also in effective utilization of heterosis. At present, cytoplasmic male sterile lines have been used in many plants, such as cotton (Gossypium hirsutum L.) [6], soybean (Glycine max L.) [7], carrot (Daucus carota L.) [8], corn (Zea mays L.) [9], onion (Allium cepa L.) [10], Brassica napus L. [11], rice (Oryza sativa L.) [4], sunflower (Helianthus annuus L.) and wheat (Triticum aestivum L.) [12,13].…”

Section: Introductionmentioning

confidence: 99%

Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil the Msβ-GAL and MsJMT as Key Candidate Genes for Cytoplasmic Male Sterility in Alfalfa (Medicago sativa L.)

Zhou

Wang

et al. 2022

IJMS

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Knowing the molecular mechanism of male sterility in alfalfa is important to utilize the heterosis more effectively. However, the molecular mechanisms of male sterility in alfalfa are still unclear. In this study, the bulked segregant analysis (BSA) and bulked segregant RNA-seq (BSR) were performed with F2 separation progeny to study the molecular mechanism of male sterility in alfalfa. The BSA-seq analysis was located in a candidate region on chromosome 5 containing 626 candidate genes which were associated with male sterility in alfalfa, while the BSR-seq analysis filtered seven candidate DEGs related to male sterility, and these candidate genes including EF-Tu, β-GAL, CESA, PHGDH, and JMT. The conjunctive analyses of BSR and BSA methods revealed that the genes of Msβ-GAL and MsJMT are the common detected candidate genes involved in male sterility in alfalfa. Our research provides a theory basis for further study of the molecular mechanism of male sterility in alfalfa and significant information for the genetic breeding of Medicago sativa.

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Cited by 33 publications

References 30 publications

De novo assembly of the carrot mitochondrial genome using next generation sequencing of whole genomic DNA provides first evidence of DNA transfer into an angiosperm plastid genome

De novo assembly of the carrot mitochondrial genome using next generation sequencing of whole genomic DNA provides first evidence of DNA transfer into an angiosperm plastid genome

Journal of Water and Land Development

Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil the Msβ-GAL and MsJMT as Key Candidate Genes for Cytoplasmic Male Sterility in Alfalfa (Medicago sativa L.)

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