DLP-based bioprinting of void-forming hydrogels for enhanced stem-cell-mediated bone regeneration

Tao, Jie; Zhu, Shunyao; Liao, Xueyuan; Wang, Yu; Zhou, Nazi; Li, Zhan; Wan, Heng; Tang, Yaping; Yang, Sen; Du, Ting; Yang, Yang; Song, Jinlin; Tao, Jie

doi:10.1016/j.mtbio.2022.100487

Cited by 20 publications

(8 citation statements)

References 43 publications

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“…It was also found that the CPP-L/GelMA hydrogel constructed by Sun et al 171 promoted bone regeneration through the Nrf2-BMAL1-autophagy pathway and was effective in scavenging ROS and regulating the bone microenvironment. Furthermore, the activation of signalling pathways such as YAP 172 , HIF1-α 173 and BMP 174 is also an important molecular mechanism for GelMA hydrogels to promote bone regeneration. Cartilage defects are a common pathogenesis of OA, and the treatment of OA is mostly based on anti-inflammatory and promotion of cartilage repair.…”

Section: Molecular Mechanism Of Gelma-based Materials For Musculoskel...mentioning

confidence: 99%

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Lv¹,

Li²,

Hu³

et al. 2023

Theranostics

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Increasing data reveals that gelatin that has been methacrylated is involved in a variety of physiologic processes that are important for therapeutic interventions. Gelatin methacryloyl (GelMA) hydrogel is a highly attractive hydrogels-based bioink because of its good biocompatibility, low cost, and photo-cross-linking structure that is useful for cell survivability and cell monitoring. Methacrylated gelatin (GelMA) has established itself as a typical hydrogel composition with extensive biomedical applications. Recent advances in GelMA have focused on integrating them with bioactive and functional nanomaterials, with the goal of improving GelMA's physical, chemical, and biological properties. GelMA's ability to modify characteristics due to the synthesis technique also makes it a good choice for soft and hard tissues. GelMA has been established to become an independent or supplementary technology for musculoskeletal problems. Here, we systematically review mechanism-of-action, therapeutic uses, and challenges and future direction of GelMA in musculoskeletal disorders. We give an overview of GelMA nanocomposite for different applications in musculoskeletal disorders, such as osteoarthritis, intervertebral disc degeneration, bone regeneration, tendon disorders and so on.

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Section: Molecular Mechanism Of Gelma-based Materials For Musculoskel...mentioning

confidence: 99%

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Lv¹,

Li²,

Hu³

et al. 2023

Theranostics

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“…DLP printing technology is widely used in bone tissue engineering owing to its good printing accuracy and gentle printing process, which requires bioink with photosensitive properties [ 37 , 38 , 39 ]. At this stage, the materials used mainly consist of natural materials (GelMA, HAMA, etc.…”

Section: Discussionmentioning

confidence: 99%

3D-Printed GelMA/PEGDA/F127DA Scaffolds for Bone Regeneration

Gao

Jin

et al. 2023

JFB

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Tissue-engineered scaffolds are an effective method for the treatment of bone defects, and their structure and function are essential for bone regeneration. Digital light processing (DLP) printing technology has been widely used in bone tissue engineering (BTE) due to its high printing resolution and gentle printing process. As commonly used bioinks, synthetic polymers such as polyethylene glycol diacrylate (PEGDA) and Pluronic F127 diacrylate (F127DA) have satisfactory printability and mechanical properties but usually lack sufficient adhesion to cells and tissues. Here, a compound BTE scaffold based on PEGDA, F127DA, and gelatin methacrylate (GelMA) was successfully prepared using DLP printing technology. The scaffold not only facilitated the adhesion and proliferation of cells, but also effectively promoted the osteogenic differentiation of mesenchymal stem cells in an osteoinductive environment. Moreover, the bone tissue volume/total tissue volume (BV/TV) of the GelMA/PEGDA/F127DA (GPF) scaffold in vivo was 49.75 ± 8.50%, higher than the value of 37.10 ± 7.27% for the PEGDA/F127DA (PF) scaffold and 20.43 ± 2.08% for the blank group. Therefore, the GPF scaffold prepared using DLP printing technology provides a new approach to the treatment of bone defects.

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“…In a similar study Tao et al formulated a void-forming bioink based on a BMSCs-loaded GelMA/dextran emulsion to enhance bone regeneration. 325 Dextran promoted spreading and proliferation of encapsulated BMSCs and enhanced the YAP signaling pathway for osteogenic differentiations of BMSCs by enhancing the void formation of DLP-bioprinted constructs.…”

Section: Ma Et Al Synthesized N-(2-aminoethyl)-4-(4-(hydroxymethyl)-mentioning

confidence: 98%

“…NIH3T3 fibroblast and HepG2 hepatocellular carcinoma cells were bioprinted along with the porogen-mixed GelMA ink, and a significantly high cell viability (>80%) was obtained. In a similar study Tao et al formulated a void-forming bioink based on a BMSCs-loaded GelMA/dextran emulsion to enhance bone regeneration . Dextran promoted spreading and proliferation of encapsulated BMSCs and enhanced the YAP signaling pathway for osteogenic differentiations of BMSCs by enhancing the void formation of DLP-bioprinted constructs.…”

Section: Modulating Scaffold Properties Through Gelma Ink Modificationsmentioning

confidence: 99%

Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

Das,

Jegadeesan,

Basu

2024

Biomacromolecules

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Tissue engineering for injured tissue replacement and regeneration has been a subject of investigation over the last 30 years, and there has been considerable interest in using additive manufacturing to achieve these goals. Despite such efforts, many key questions remain unanswered, particularly in the area of biomaterial selection for these applications as well as quantitative understanding of the process science. The strategic utilization of biological macromolecules provides a versatile approach to meet diverse requirements in 3D printing, such as printability, buildability, and biocompatibility. These molecules play a pivotal role in both physical and chemical cross-linking processes throughout the biofabrication, contributing significantly to the overall success of the 3D printing process. Among the several bioprintable materials, gelatin methacryloyl (GelMA) has been widely utilized for diverse tissue engineering applications, with some degree of success. In this context, this review will discuss the key bioengineering approaches to identify the gelation and cross-linking strategies that are appropriate to control the rheology, printability, and buildability of biomaterial inks. This review will focus on the GelMA as the structural (scaffold) biomaterial for different tissues and as a potential carrier vehicle for the transport of living cells as well as their maintenance and viability in the physiological system. Recognizing the importance of printability toward shape fidelity and biophysical properties, a major focus in this review has been to discuss the qualitative and quantitative impact of the key factors, including microrheological, viscoelastic, gelation, shear thinning properties of biomaterial inks, and printing parameters, in particular, reference to 3D extrusion printing of GelMA-based biomaterial inks. Specifically, we emphasize the different possibilities to regulate mechanical, swelling, biodegradation, and cellular functionalities of GelMA-based bio(material) inks, by hybridization techniques, including different synthetic and natural biopolymers, inorganic nanofillers, and microcarriers. At the close, the potential possibility of the integration of experimental data sets and artificial intelligence/machine learning approaches is emphasized to predict the printability, shape fidelity, or biophysical properties of GelMA bio(material) inks for clinically relevant tissues.

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DLP-based bioprinting of void-forming hydrogels for enhanced stem-cell-mediated bone regeneration

Cited by 20 publications

References 43 publications

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment

3D-Printed GelMA/PEGDA/F127DA Scaffolds for Bone Regeneration

Gelatin Methacryloyl (GelMA)-Based Biomaterial Inks: Process Science for 3D/4D Printing and Current Status

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