DlS80 allele frequencies in Hasidic and non-Hasidic New York City Jewish populations

Medintz, Igor L.; Kingston, Charles; Levine, Louis; Fogarty, Patrick F.; Mar, Eng-Chun; McCurdy, Leslie D.; Kobilinsky, Lawrence

doi:10.1007/s004140050168

Cited by 3 publications

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“…The routine genetic characterization of 96 samples from the New York City Ashkenazi Jewish population at the D1S80 locus (Medintz et al, 1998) revealed the presence of an extra amplicon that corresponded in size to a D1S80 allele consisting of 15 repeat units. This allele was found in eight samples.…”

Section: Resultsmentioning

confidence: 99%

“…All samples were collected with informed consent that they be used anonymously for other research purposes. The origin and ethnicity of these samples have been described previously (Medintz et al, 1997(Medintz et al, , 1998. Ninety-six samples were obtained and typed for this study.…”

Section: Samplesmentioning

confidence: 99%

“…The routine genetic characterization of the New York City Ashkenazi Jewish population at the D1S80 locus (Medintz et al, 1998) revealed the presence of a third band corresponding in position to a D1S80 15 allele in seven samples. An additional sample appeared to be homozygous for the #15 allele.…”

Section: Introduction O Ne Of the Most Widely Used Marker Systems In mentioning

confidence: 99%

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Characterization of a Novel D1S80 Pseudoallele

et al. 2000

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During a D1S80 population study conducted for databasing purposes in the New York City Ashkenazi Jewish population, eight out of 96 samples were typed with a band corresponding to the position of a #15 allele. In seven of the eight samples, three bands appeared. Further investigation was needed to explain the high frequency of an allele considered so rare that it is not included in the commercially provided allelic ladder. After extraction of the putative D1S80 15-repeat amplicon band from the 6% polyacrylamide genotyping gel, the amplicon bands were reamplified with D1S80 primers. After retyping as putative 15 alleles, these samples underwent Southern hybridization with a D1S80 locus-specific probe followed by DNA sequence analysis. Sequence analysis revealed that these bands did not arise from true D1S80 15 alleles. However, the PCR product was of a size that fell within the allelic ladder region corresponding to the 15 band and contained end sequences with strong homology to the D1S80 primers. An alignment search of the sequenced product revealed that a portion of the amplicons contained 72% identity to a known gene. These results emphasize the importance of sequencing analysis when questions arise about the authenticity of an allele.

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Section: Resultsmentioning

confidence: 99%

Section: Samplesmentioning

confidence: 99%

Section: Introduction O Ne Of the Most Widely Used Marker Systems In mentioning

confidence: 99%

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