DNA-aptamer-nanographene oxide as a targeted bio-theragnostic system in antimicrobial photodynamic therapy against Porphyromonas gingivalis

Pourhajibagher, Maryam; Etemad‐Moghadam, Shahroo; Alaeddini, Mojgan; Mousavi, Rezvaneh Sadat Miri; Bahador, Abbas

doi:10.1038/s41598-022-16310-3

Cited by 19 publications

(12 citation statements)

References 67 publications

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“…Zinc oxide functionalized graphene oxide polyetheretherketone was reported as an antibacterial agent against periodontal pathogens and biofilms [ 101 , 102 ]. Another study demonstrated that DNA-aptamer-nanographene oxide was highly effective in reducing Porphyromonas gingivalis [ 103 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review

et al. 2022

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Graphene is a single-layer two-dimensional carbon-based nanomaterial. It presents as a thin and strong material that has attracted many researchers’ attention. This study provides a concise review of the potential application of graphene materials in caries and periodontal disease management. Pristine or functionalized graphene and its derivatives exhibit favorable physicochemical, mechanical, and morphological properties applicable to biomedical applications. They can be activated and functionalized with metal and metal nanoparticles, polymers, and other small molecules to exhibit multi-differentiation activities, antimicrobial activities, and biocompatibility. They were investigated in preventive dentistry and regenerative dentistry. Graphene materials such as graphene oxide inhibit cariogenic microbes such as Streptococcus mutans. They also inhibit periodontal pathogens that are responsible for periodontitis and root canal infection. Graphene-fluorine promotes enamel and dentin mineralization. These materials were also broadly studied in regenerative dental research, such as dental hard and soft tissue regeneration, as well as periodontal tissue and bone regeneration. Graphene oxide-based materials, such as graphene oxide-fibroin, were reported as promising in tissue engineering for their biocompatibility, bioactivity, and ability to enhance cell proliferation properties in periodontal ligament stem cells. Laboratory research showed that graphene can be used exclusively or by incorporating it into existing dental materials. The success of laboratory studies can translate the application of graphene into clinical use.

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Section: Resultsmentioning

confidence: 99%

In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review

et al. 2022

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“…The DNA-aptamer-NGO presents an intrinsic antibacterial activity and increases under irradiation. Their action mechanism reduces bacterial metabolic activity, as observed in Figure 3b, which leads to a higher rate of bacterial apoptosis as a function of concentration and irradiation (see Figure 3c) [71]. In APDT with GO, there is a dependence between the light dose and the loss of bacterial viability, for example, for the bacteria E. coli, its loss of viability is aggravated for 40-60 J⋅cm −2 at higher concentrations.…”

Section: Graphene Oxide and Reduced-graphene Oxide In Apdtmentioning

confidence: 93%

“…That is, there is a threshold of light dose and concentrations of the PS, where the photons are available to excite GO and generate 1 O 2 and the ROS is optimal for causing bacterial death and avoiding affecting healthy tissues. gingivalis with DNA-aptamer-NGO in APDT [71]. (c) The apoptosis rate of P. gingivalis with DNA-aptamer-NGO in APDT [71].…”

Section: Graphene Oxide and Reduced-graphene Oxide In Apdtmentioning

confidence: 99%

“…Figure 3. (a) Bacterial viability of P. gingivalis with DNA-aptamer-NGO in APDT[71]. (b) Metabolic activity of P. gingivalis with DNA-aptamer-NGO in APDT[71].…”

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confidence: 99%

“…(a) Bacterial viability of P. gingivalis with DNA-aptamer-NGO in APDT[71]. (b) Metabolic activity of P. gingivalis with DNA-aptamer-NGO in APDT[71]. (c) The apoptosis rate of P. gingivalis with DNA-aptamer-NGO in APDT[71].…”

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Antibacterial Strategies: Photodynamic and Photothermal Treatments Based on Carbon-Based Materials

Vélez¹,

Álvarez²,

Obando³

2023

Biomedical Engineering

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The problem of bacterial resistance is based on the abuse of antibiotics such as trimethoprim, fluoroquinolones, chloramphenicol, and some carbapenems. For this reason, conventional treatments to treat diseases caused by bacteria have become ineffective. Therefore, developing new therapies with multifunctional materials to combat bacteria is mandatory. In this context, photodynamic treatment (PDT) and photothermal treatment (PTT) have been proposed to combat bacteria. These light-stimulated treatments are minimally invasive and have a low incidence of side effects. In addition, they are simple, fast, and profitable. The antibacterial effect of PDT, PTT, or synchronic PDT/PTT arises from the generation of reactive oxygen species (ROS) and heat caused by a photoactivated specific photosensitizer (PS) and photothermal agents (PTAs), respectively. The effectiveness of photoinduced treatment depends, among other parameters, on the nature and concentration of the PS/PTAs, light dose, and irradiation wavelength. PS/PTAs based on carbon-based materials (CBMs), such as graphene oxide, reduced graphene oxide, carbon dots, and carbon nanotubes as antibacterial agents, will be discussed in this chapter. These CBMs have emerged as excellent antibacterial alternatives due to their excellent physicochemical properties, biocompatibility, low toxicity in the dark, specificity, and excellent response to light. Moreover, several composites and hybrids employing polymers, metal oxides, and metals have been tested to enhance the antibacterial activity of the CBMs.

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Screening and characterization of aptamers recognizing the periodontal pathogen Tannerella forsythia

Mizgalska,

Malicki,

Golda

et al. 2024

FEBS Open Bio

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Periodontal disease is one of the most common forms of inflammation. It is currently diagnosed by observing symptoms such as gingival bleeding and attachment loss. However, the detection of biomarkers that precede such symptoms would allow earlier diagnosis and prevention. Aptamers are short oligonucleotides or peptides that fold into three‐dimensional conformations conferring the ability to bind molecular targets with high affinity and specificity. Here we report the selection of aptamers that bind specifically to the bacterium Tannerella forsythia, a pathogen frequently associated with periodontal disease. Two aptamers with the highest affinity were examined in more detail, revealing that their binding is probably dependent on mirolysin, a surface‐associated protease secreted by the T. forsythia type‐9 secretion system. The aptamers showed minimal cross‐reactivity to other periodontopathogens and are therefore promising leads for the development of new tools to study the composition of the periodontitis‐associated dysbiotic bacteriome as well as inexpensive new diagnostic assays.

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DNA-aptamer-nanographene oxide as a targeted bio-theragnostic system in antimicrobial photodynamic therapy against Porphyromonas gingivalis

Cited by 19 publications

References 67 publications

In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review

In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review

Antibacterial Strategies: Photodynamic and Photothermal Treatments Based on Carbon-Based Materials

Screening and characterization of aptamers recognizing the periodontal pathogen Tannerella forsythia

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