DNA-arrays with electrical detection: A label-free low cost technology for routine use in life sciences and diagnostics

Liepold, Petra; Wieder, H.; Hillebrandt, Heiko; Friebel, A.; Hartwich, Gerhard

doi:10.1016/j.bioelechem.2004.08.004

Cited by 40 publications

(20 citation statements)

References 44 publications

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“…Some of the common methods for modifying individual electrodes within an array include spotting, inkjet deposition, and photo directed synthesis processes (Liepold et al, 2005). The novelty of using an electrically addressable deposition procedure of proteins for multianalyte detection in an array format is demonstrated.…”

Section: Multi-cytokine Detection On An Electrode Arraymentioning

confidence: 99%

Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications

Polsky

Harper

Wheeler

et al. 2008

Biosensors and Bioelectronics

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Section: Multi-cytokine Detection On An Electrode Arraymentioning

confidence: 99%

Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications

Polsky

Harper

Wheeler

et al. 2008

Biosensors and Bioelectronics

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“…This sensitivity is largely linked to the enzymes used for molecular detection and future developments in molecular virus diagnostics may therefore increasingly feature enzyme-free technologies (Liepold et al, 2005). and host.…”

Section: Future Perspectivesmentioning

confidence: 99%

Standard methods for virus research inApis mellifera

Miranda

Bailey

Ball

et al. 2013

Journal of Apicultural Research

272

229

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SummaryHoney bee virus research is an enormously broad area, ranging from subcellular molecular biology through physiology and behaviour, to individual and colony-level symptoms, transmission and epidemiology. The research methods used in virology are therefore equally diverse.This article covers those methods that are very particular to virological research in bees, with numerous cross-referrals to other BEEBOOK papers on more general methods, used in virology as well as other research. At the root of these methods is the realization that viruses at their most primary level inhabit a molecular, subcellular world, which they manipulate and interact with, to produce all higher order phenomena associated with virus infection and disease. Secondly, that viruses operate in an exponential world, while the host operates in a linear world and that much of the understanding and management of viruses hinges on reconciling these fundamental mathematical differences between virus and host. The article concentrates heavily on virus propagation and methods for detection, with minor excursions into surveying, sampling management and background information on the many viruses found in bees. Métodos estándar para la investigación de virus en Apis mellifera ResumenLa investigación de los virus de la abeja de la miel es un área sumamente amplia, que abarca desde la biología molecular subcelular hasta la fisiología y el comportamiento, desde síntomas al nivel de individuo hasta al nivel de la colmena, transmisión y epidemiología. Los métodos de investigación en virología son, por tanto, diversos. Este artículo incluye aquellos métodos específicos de la investigación virológica en las abejas, con numerosas referencias cruzadas con otros artículos del BEEBOOK y otros más generales, usados tanto en virología como en otras disciplinas. La base de estos métodos es la comprensión de los virus en su nivel primario de hábitat molecular, ambiente subcelular, que manipulan y con el que interactúan, para producir otros fenómenos de orden superior asociados a la infección del virus y la enfermedad. En segundo lugar, estos virus actúan en un mundo exponencial, mientras que los hospedadores actúan en un mundo lineal y gran parte del entendimiento y manejo de los virus depende de los fundamentos matemáticos de las diferencias entre el virus y el hospedador. El artículo se centra principalmente en la propagación de virus y en los métodos para su detección, con inclusiones menores en su estudio, el manejo del muestreo y la información general sobre los numerosos virus que se encuentran en las abejas.

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“…This approach eliminates the need for additional sample separation processes or reporting molecules and allows for DNA levels to be monitored real-time within biological systems. Label-free detection can be carried out without any sort of labeling (9, 13, 49), or can incorporate a labeled reporter in conjunction with non-labeled targets (10, 50). …”

Section: Electrochemical Dna Sensor Typesmentioning

confidence: 99%

“…While offering simplicity in operation and sample manipulation, the contemporary electrochemical biosensor also provides highly sensitive and specific measurements for a broad spectrum of biomolecules (13–17). The sample size required for current EC sensors is small, ranging from several microliters to hundreds of nanoliters, which includes the sample pretreatment reagents.…”

Section: Introductionmentioning

confidence: 99%

DNA Diagnostics: Nanotechnology-Enhanced Electrochemical Detection of Nucleic Acids

2010

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ABSTRACT:The detection of mismatched base pairs in DNA plays a crucial role in the diagnosis of genetic-related diseases and conditions, especially for early stage treatment. Among the various biosensors that have been used for DNA detection, EC sensors show great promise because they are capable of precise DNA recognition and efficient signal transduction. Advancements in micro-and nanotechnologies, specifically fabrication techniques and new nanomaterials, have enabled for the development of highly sensitive, highly specific sensors making them attractive for the detection of small sequence variations. Furthermore, the integration of sensors with sample preparation and fluidic processes enables for rapid, multiplexed DNA detection essential for POC clinical diagnostics. T he recent discovery and sequencing of the human genome has provided valuable insight into understanding how genetic factors contribute to the development of disease. Specifically, the detection of DNA sequence variations plays an important role in the diagnosis of genetic-related diseases and conditions, especially for early stage treatment and monitoring. Among the different types of diseases caused by DNA alterations, sequence-specific mismatch has the most importance, yet is extremely difficult to detect (1), especially for single-nucleotide polymorphism (SNP). Furthermore, sequence-specific detection has great importance in various medical and scientific applications such as the diagnosis of inherited diseases and the study of pathogen response and bacterial/viral detection.Because of the complex nature of DNA, the detection of single or small numbers of base mismatches requires high sensitivity and specificity (2-4). Current detection methods rely on sample amplification combined with meticulous experimental stringency control (5). For example, polymerase chain reaction (PCR) requires careful primer design and accurate temperature control to obtain sensitivities in the fM range with single-base mismatch specificity (1,3,4). Although these conventional technologies provide the golden standard for laboratory-based DNA diagnostics, they cannot meet the requirements of POC clinical diagnostics (4).EC sensors, initially developed to detect biomolecules in a laboratory setting, have recently found extensive applications for on-site biosensing and detection (6,7), especially for medical and clinical diagnostics (8 -12). While offering simplicity in operation and sample manipulation, the contemporary EC biosensor also provides highly sensitive and specific measurements for a broad spectrum of biomolecules (13-17). The sample size required for current EC sensors is small, ranging from several microliters to hundreds of nanoliters, which includes the sample pretreatment reagents. Additionally, the detection time is relatively fast, varying from a few minutes to tens of seconds. However, the most important feature of EC sensors is their potential to be easily transformed from a laboratory-based instrument to a commercializable POC device. Because o...

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DNA-arrays with electrical detection: A label-free low cost technology for routine use in life sciences and diagnostics

Cited by 40 publications

References 44 publications

Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications

Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications

Standard methods for virus research inApis mellifera

DNA Diagnostics: Nanotechnology-Enhanced Electrochemical Detection of Nucleic Acids

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