DNA barcode assessment of Mediterranean mayflies (Ephemeroptera), benchmark data for a regional reference library for rapid biomonitoring of freshwaters

The imagos of some enigmatic members of the Hermanella complex (Ephemeroptera, Leptophlebiidae)

“…Sequence differences smaller than 3% are frequently observed in intraspecific distances of DNA barcodes (Ferguson 2002; Hebert et al 2003; Hebert et al 2004; Ball et al 2005; Cardoni et al 2015; Gattolliat et al 2015; Angeli et al 2016). Whereas the examined specimens of Hydromastodon sallesi for the barcode analysis were from the type-locality and those of Leentvaaria palpalis were from an area relatively close to its type-locality, the specimens of Hydrosmilodon gilliesae were from southeastern Brazil.…”

Baetis (Baetis) cypronyx sp. n., a new species of the Baetis alpinus species-group (Insecta, Ephemeroptera, Baetidae) from Cyprus, with annotated checklist of Baetidae in the Mediterranean islands

“…So far no morphological differences have been determined for these putative species. The high intra-specific genetic distance in Baetis cyrneus recently detected by Cardoni et al (2015) for populations from Corsica and Sardinia also point to cryptic variation.3Recorded by Belfiore and D’Antonio (1991) and Belfiore et al (1991) for the first time.4Recorded by Belfiore and Gaino (1988: 77) for the first time in Sardinia and later also listed from Sicily (Belfiore and D’Antonio 1991; Buffagni et al 2003); for Corsica based on OPIE-benthos data.5So far known only from type locality and a single additional locality in Rhodos (Soldán and Godunko 2009: 7−8), considered endemic to the island.6The record from Kos by Belfiore (1990: 266) probably belongs to or other, still undescribed species of the Baetis lutheri species-group.7Considered as probably East Mediterranean (Pontomediterranean) species by Bauernfeind and Soldán (2012: 124); so far known from three localities in Cyprus and from a single locality in Rhodos (Soldán and Godunko 2008: 95).8Recorded by Belfiore (1983) for the first time; recent data on distribution summarized by Belfiore and D’Antonio (1991: 260), Belfiore et al (1991: 32) and Buffagni et al (2003: 281).9Most probably missing on Mediterranean islands (Bauernfeind and Soldán 2012: 136). The record for Corsica by Sartori and Thomas (1989: 131) based on earlier data by Verrier (1954: 282 [sub.…”

“…For Sicily and Sardinia see summarized data in Belfiore (1983), Belfiore and D’Antonio (1991), Belfiore et al (1991) and Buffagni et al (2003). Recent data on DNA barcoding by Cardoni et al (2015) based on Sardinian material.11The species can be considered endemic to Corsica. This conclusion is confirmed by recent DNA barcoding (Gattolliat et al 2015; Cardoni et al 2015).…”

“…Cloeon gr. simile] needs to be clarified. Recently DNA barcoded by Cardoni et al (2015).28So far known from several localities in Sicily and Sardinia (Belfiore and D’Antonio 1991; Buffagni et al 2003; Bauernfeind and Soldán 2012). Reported from Elba and Sardinia by Cardoni et al (2015), with remarks on its possible presence in the Corse-Sardinian biogeographic region; based on DNA barcoding the specimens from Elba and Sardinia may however represent a cryptic endemic species as they differ significantly from specimens of Continental Europe (Cardoni et al 2015).…”

Development of genomic resources for four potential environmental bioindicator species:Isoperla grammatica,Amphinemura sulcicollis,Oniscus asellus and Baetis rhodani

“…Macroinvertebrates are widespread, often dominant and functionally important members of their environment that, coupled with their relative ease of sampling make them ideally suited for use as indicator species for biomonitoring and conservation assessment (Pfrender et al 2010; Buss et al 2015;Cardoni et al 2015), and are recognised as such by the Water Framework Directive (2000/60/CE) (European Commission 2000). However, their use in genetic approaches is still limited, often being neglected from studies because of the lack of data (Cardoso et al 2011).…”