DNA barcodes confirm the presence of a single member of the Anopheles maculipennis group in Morocco and Algeria: An. sicaulti is conspecific with An. labranchiae

Laboudi, Majda; Faraj, Chafika; Sadak, Abderrahim; Harrat, Zoubir; Boubidi, S. C.; Harbach, Ralph E.; Aouad, Rajae El; Linton, Yvonne‐Marie

doi:10.1016/j.actatropica.2010.12.006

Cited by 24 publications

(15 citation statements)

References 30 publications

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“…The discovery of cryptic species in Culicidae also entails serious public health implications as it directly impacts the design of vector control and management programs (Bickford et al, 2007). The correct identification of the species is essential for the development of programs for the control and prevention of diseases transmitted by mosquitoes, as it allows focusing only on the control of those species that transmit certain diseases (Azari-Hamidian et al, 2010;Bueno-Marí, Corella-López, & Jiménez-Peydró, 2010;Erlank, Koekemoer, & Coetzee, 2018;Laboudi et al, 2011).…”

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confidence: 99%

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Chan-Chablé

Martínez‐Arce

Mis-Ávila³

et al. 2019

Ecology and Evolution

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Culicidae mosquitoes are potential vectors of pathogens that affect human health. The correct species identification, as well as the discovery and description of cryptic species, is important in public health for the control and management of specific vectors. In the present study, the diversity of anthropophagous mosquitoes in Quintana Roo, at the border between Mexico and Belize, was evaluated using morphological and molecular data (COI‐DNA Barcoding). A total of 1,413 adult female specimens were collected, belonging to eight genera and 31 morphospecies. Most species formed well‐supported clades. Intraspecific Kimura 2 parameters (K2P) distance average was 0.75%, and a maximum distance of 4.40% was observed for Anopheles crucians s.l. ABGD method identified 28 entities, while 32 entities were identified with the BIN system. In Culex interrogator and Culex nigripalpus a low interspecific genetic distance of 0.1% was observed. One undescribed species belonging to the genus Aedes ( Aedes n. sp.) was discovered, but no clear genetic divergence was found between this species and the closely related species Aedes angustivittatus . An intraspecific K2P distance greater than 2.7% was observed in Aedes serratus (3.9%), Anopheles crucians s.l. (4.4%), Culex taeniopus (3.7%), Haemagogus equinus (3.9%), Culex erraticus (5.0%), Psorophora ferox (4.5%), and in Anopheles apicimacula (8.10%); therefore, evidences of cryptic diversity are shown in these species. This study showed that DNA barcodes offer a reliable framework for mosquito species identification in Quintana Roo, except for some closely related species for which it is recommended to use additional nuclear genetic markers such as ITS2, in order to resolve these small discrepancies.

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confidence: 99%

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Chan-Chablé

Martínez‐Arce

Mis-Ávila³

et al. 2019

Ecology and Evolution

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“…labranchiae is somewhat unusual, in that it is believed to be the only African member of the Maculipennis complex. It is highly abundant and widespread in the Maghreb countries: Morocco, 22,[36][37][38][39][40] Algeria, 22,41,42 and Tunisia. 10,35 It is assumed that An.…”

Section: Discussionmentioning

confidence: 99%

“…15,16 Recent studies based on the analysis of DNA sequences have leveraged part of the problem by providing a straight-forward and reliable polymerase chain reaction (PCR)-based molecular identification tool that allows species discrimination within the Maculipennis group in many countries, including Italy, Romania, Iran, England, Algeria, Morocco, and Greece. 11,13,14,[17][18][19][20][21][22][23][24][25][26][27][28] These studies have retained An. labranchiae as the only member of the Maculipennis complex found in Morocco and Algeria, 22 whereas species composition within the Maculipennis complex is still pending for Tunisia.…”

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confidence: 99%

Larval Habitats Characterization and Species Composition of Anopheles Mosquitoes in Tunisia, with Particular Attention to Anopheles maculipennis Complex

Tabbabi¹,

Boussès²,

Rhim³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…Both molecular markers have been employed to test hypotheses of molecular taxonomy and systematics in malaria vectors using phylogenetic analysis (Wilkerson et al, 2005; Marrelli et al, 2006; Li and Wilkerson, 2007; Paredes-Esquivel et al, 2009; Bourke et al, 2010; Ruiz-Lopez et al, 2012), to investigate patterns of population structure, and to validate species distribution records (González et al, 2010; Loaiza et al, 2010b; 2012; Laboudi et al, 2011). The ITS2 is recognized as the gold standard for species identification in most Anopheles sibling complexes; length differences and fixed substitutional changes between ITS2 sequences are taken as proof of lineage splitting especially if the lineages are geographically co-distributed (Walton et al, 2007a,b).…”

Section: Introductionmentioning

confidence: 99%

Novel genetic diversity within Anopheles punctimacula s.l.: Phylogenetic discrepancy between the Barcode cytochrome c oxidase I (COI) gene and the rDNA second internal transcribed spacer (ITS2)

et al. 2013

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Anopheles punctimacula s.l. is a regional malaria vector in parts of Central America, but its role in transmission is controversial due to its unresolved taxonomic status. Two cryptic species, An. malefactor and An. calderoni, have been previously confused with this taxon, and evidence for further genetic differentiation has been proposed. In the present study we collected and morphologically identified adult female mosquitoes of An. punctimacula s.l. from 10 localities across Panama and one in Costa Rica. DNA sequences from three molecular regions, the three prime end of the mitochondrial cytochrome c oxidase I gene (3´ COI), the Barcode region in the five prime end of the COI (5´ COI), and the rDNA second internal transcribed spacer (ITS2) were used to test the hypothesis of new molecular lineages within An. punctimacula s.l. Phylogenetic analyses using the 3´ COI depicted six highly supported molecular lineages (A–F), none of which was An. malefactor. In contrast, phylogenetic inference with the 5´ COI demonstrated paraphyly. Tree topologies based on the combined COI regions and ITS2 sequence data supported the same six lineages as the 3´ COI alone. As a whole this evidence suggests that An. punctimacula s.l. comprises two geographically isolated lineages, but it is not clear whether these are true species. The phylogenetic structure of the An. punctimacula cluster as well as that of other unknown lineages (C type I vs C type II; D vs E) appears to be driven by geographic partition, because members of these assemblages did not overlap spatially. We report An. malefactor for the first time in Costa Rica, but our data do not support the presence of An. calderoni in Panama.

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DNA barcodes confirm the presence of a single member of the Anopheles maculipennis group in Morocco and Algeria: An. sicaulti is conspecific with An. labranchiae

Cited by 24 publications

References 30 publications

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Larval Habitats Characterization and Species Composition of Anopheles Mosquitoes in Tunisia, with Particular Attention to Anopheles maculipennis Complex

Novel genetic diversity within Anopheles punctimacula s.l.: Phylogenetic discrepancy between the Barcode cytochrome c oxidase I (COI) gene and the rDNA second internal transcribed spacer (ITS2)

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