DNA Barcoding and Phylogenetic Placement of the Genus Euphorbia L. (Euphorbiaceae) in Egypt

El-Banhawy, Ahmed; Uluer, Deniz Aygören; Fayed, Abdel Aziz; Mohamed, Mona H.; Faried, Ahmed

doi:10.3390/iecps2020-08620

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…Similarly, the sequence match with the MatK loci was from 96.25 to 100%. These results are in contrast with the phylogenetic study conducted by El-Banhawy, 2020 in the genus Euphorbia . It was reported that the rbcl was the least successful and matK genes were not significantly different in identifying the Egyptian Euphorbia.…”

Section: Discussioncontrasting

confidence: 86%

“…These results are in contrast with the phylogenetic study conducted by El-Banhawy, 2020 in the genus Euphorbia. 37 It was reported that the rbcl was the least successful and matK genes were not significantly different in identifying the Egyptian Euphorbia. However, the results of this study reveal a good identity score of 99.84 and 100 for Euphorbia plant grown in India.…”

Section: Discussionmentioning

confidence: 97%

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Standardization of Euphorbia tithymaloides (L.) Poit. (Root) by Conventional and DNA Barcoding Methods

Patil,

Imran,

Jaquline

et al. 2023

ACS Omega

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Adulteration and substitution of medicinal plants have become a matter of great concern in recent years. Euphorbia tithymaloides is one such medicinal plant that has gained importance but is often confused with other plants of the same species. In order to address this issue, this study aimed to conduct a conventional and molecular pharmacognostic study for the identification of the root of E. tithymaloides. The root of the plant was studied for the macroscopic observations, and then, the root was ground into coarse powder for microscopic studies and to determine the physiochemical properties. The powder was subjected to extraction with solvents such as ethanol, ethanol/water (1:1), hexane, and ethyl acetate. The extracts were then used for qualitative and quantitative (phenol, alkaloids, and flavonoids) phytochemical analysis. The molecular study was performed with the DNA barcoding technique. The DNA was extracted from the root of the plant, and its purity was examined by gel electrophoresis (1% w/v). The DNA was then amplified using an Applied Biosystems 2720 thermal cycler for the rbcL, matK, and ITS primers. The amplified primers were sequenced with a 3130 Genetic Analyzer, and the generated sequences were searched for similarity in the GenBank Database using the nucleotide BLAST analysis. The micro- and macroscopic studies revealed the morphological and organoleptic characters as well as the presence of medullary rays, fiber, cork, sclereids, parenchymal cells, and scalariform vessels. The physiochemical properties were found within the limit. The phytochemical analysis revealed the presence of terpenoids, flavonoids, saponins, and alkaloids. In addition, the alkaloidal content was high in the ethanol extract (63.04 ± 3.08 mg At E/g), while the phenol content was high in the hexane extract (10.26667 ± 1.77 mg At E/g), and the flavonoid content was high in the ethyl acetate extract (41.458 ± 1.33 mg At E/g). After the BLAST analysis from the GenBank database, the rbcL, ITS, and matK primers showed a similarity percentage of 99.83, 99.84, and 100. The phylogenetic tree for the species closest to each primer was generated using the MEGA 6 software. The matK loci had the highest percentage similar to the rbcL and ITS loci, indicating that the matK loci can be used to identify the root of E. tithymaloides as a standalone. The results from this study can be used to establish a quality standard for E. tithymaloides that will ensure its quality and purity.

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Section: Discussioncontrasting

confidence: 86%

Section: Discussionmentioning

confidence: 97%

Standardization of Euphorbia tithymaloides (L.) Poit. (Root) by Conventional and DNA Barcoding Methods

Patil,

Imran,

Jaquline

et al. 2023

ACS Omega

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“…Furthermore, Al-Nafie [49] mentioned that the existence of a certain number of species corresponding to different taxa is a salient characteristic of this region, showing high plant diversity. Members of the recognized families are also well known for their higher economic and medicinal potentiality [50][51][52][53][54][55][56][57][58]. Anthemis scrobicularis was the only endemic species recorded in the surveyed area [59].…”

Section: Discussionmentioning

confidence: 99%

Floristic Diversity of Jabal Al-Ward, Southwest Tabuk Region, Kingdom of Saudi Arabia

et al. 2022

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Jabal Al-Ward is one of the Hijazi mountains situated between Al-Ulā and Al-Wajh, southwest Tabuk Province, Saudi Arabia’s northwesterly border region. It is considered the highest mountain in this area and is enriched in wildlife. For the first time, the present research aimed to investigate the floristic composition, phytogeographical distribution, and plant diversity in Jabal Al-Ward. One hundred ninety-eight species representing 47 plant families have been identified. The Asteraceae, Poaceae, and Fabaceae represented more than a third of the region’s floristic composition. The perennial species (53.5%) were dominant over the annuals (46.46%). This is a prominent feature in Jabal Al-Ward, where the perennial species may be more tolerant of climatic changeability than the annuals. Seven life form categories were found; therophytes (46.46%) showed to be the most common life form. In addition, there were four main phytogeographical groups: Mono-regional, Bi-regional, Pluri-regional, and Worldwide. The Mono-regional and Bi-regional categories had the highest participation, with 38.5% and 37.4%, respectively. Thirty-six species (18.2%) were found to be native to the Saharo-Arabian region. The Saharo-Arabian region was combined with eight more regions, including Saharo-Arabian/Sudano-Zambesian (12.6%), Irano-Turanian/Saharo-Arabian (9.1%), Mediterranean/Saharo-Arabian (5.6%), Irano-Turanian/Mediterranean/Saharo-Arabian (4.5%), Irano-Turanian/Saharo-Arabian/Sudano-Zambesian (2%), Euro-Siberian/Irano-Turanian/Mediterranean/Saharo-Arabian and Saharo-Arabian/Sudanian (1% each), Mediterranean/Saharo-Arabian/Sudano-Zambesian, and Irano-Turanian/Mediterranean/Saharo-Arabian/Saharo-Zambesian (0.5% each). The current study demonstrated the highest species richness compared to earlier research on various locations in Tabuk Province. In future work, the species and endemic richness along elevation gradients should be studied in Jabal Al-Ward. As well as the IUCN status of each taxon, the DNA barcoding of endangered species will be of great significance if applied in the surveyed area.

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“…Moreover, leaf indumentum should be a helpful tool for the same purpose [16][17][18][19][20][21]. Recently, DNA barcoding has been used extensively to resolve the classification of many proplomatic taxa [22][23][24]. DNA barcoding facilitates fast species identification based on standardized and short DNA sequences from organelle genomes or interspacer ribosomal genes [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Anatomical and Molecular Identification of Ornamental Plant Ficus L. Species

Binnoubah¹,

Hamdy²,

Ragab³

et al. 2023

Phyton

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This present study includes twelve species that represent the Ficus genus, namely; aspera, carica, tinctoria subsp. gibbosa, hirta, hispida, neriifolia, palmata, pumila, racemosa, septica, sur, and sycomorus, belonging to the Moraceae family. The species samples were collected from various locations in Egypt. The study focused on the anatomical and molecular characteristics of mature foliage leaves. Since the identification and classification of taxa are highly dependent on the anatomical features of leaves, the anatomical characteristics were recorded in the form of a comparison between the examined plants in the data matrix. This study aims to contribute to the identification of the studied species based on the anatomical details of the matured leaves. Anatomical characterization includes the variations in upper and lower epidermal layers that are covered by a thin or thick cuticle; the number of palisade and spongy layers; crystals; secretory elements; lithocysts; the midrib zone has parenchyma associated with mechanical tissue, vascular system, and investigation of trichomes; on the other hand, in the current study, the phylogenetic analysis was conducted by using the ITS and 5.8 S sequences. From the analysis of all the available data, it could be stated that there is an overall agreement with the anatomical character dendrogram.

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DNA Barcoding and Phylogenetic Placement of the Genus Euphorbia L. (Euphorbiaceae) in Egypt

Cited by 3 publications

References 31 publications

Standardization of Euphorbia tithymaloides (L.) Poit. (Root) by Conventional and DNA Barcoding Methods

Standardization of Euphorbia tithymaloides (L.) Poit. (Root) by Conventional and DNA Barcoding Methods

Floristic Diversity of Jabal Al-Ward, Southwest Tabuk Region, Kingdom of Saudi Arabia

Anatomical and Molecular Identification of Ornamental Plant Ficus L. Species

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