DNA barcoding application of garfish (Hemirhampus sp.) in North Maluku Sea

Achmad, M. Janib; Djamhur, Martini; Fabanyo, M. Abjan; Akbar, Nebuchadnezzar

doi:10.32491/jii.v19i3.506

Cited by 3 publications

(6 citation statements)

References 6 publications

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“…However, the diversity and number of Hemiramphus species living in this area are still unknown. The presence of Hemiramphus in Indonesia has been reported by several studies (Kawimbang et al 2012;Suryawan et al 2016;Ahmad et al 2019;Natan et al 2019). Previously, fish identification was conducted mainly by a morphological approach based on morphometric and meristic characteristics.…”

Section: Introductionmentioning

confidence: 79%

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Record of Hemiramphus lutkei Valenciennes, 1847 from Seram Sea, Maluku, Indonesia with its molecular characterization

Talakua

Suadi²,

Djumanto³

et al. 2022

Biodiversitas

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Abstract. Talakua M, Suadi, Djumanto, Setyobudi E. 2022. Record of Hemiramphus lutkei Valenciennes, 1847 from Seram Sea, Maluku, Indonesia with its molecular characterization. Biodiversitas 23: 1560-1566. Hemiramphus sp. is a fish species widely distributed throughout the world. However, the area distribution of each species differs between regions. Hemiramphus archipelagicus could be found in Seram Sea, Maluku and Ekas Bay, Lombok, Hemiramphus sp. from North Maluku Sea, and H. brasiliensis from the North Minahasa Sulawesi. The identification of Hemiramphus in Indonesia uses mainly the morphological approach. This study aims to determine the morphometric and meristic character and identify the halfbeak collected from the Seram Sea, Maluku, using a molecular approach. A total of 214 fish samples were used to determine the morphometric and meristic characters of halfbeak, following the measurement method that has been developed previously. All samples of halfbeak showed similar morphological characteristics. Identification by direct sequencing of the cytochrome c oxidase subunit I (COI) gene was conducted from selected samples. The halfbeak caught in the Seram Sea had an elongated flat body shape, the dorsal part of the body is blue-green, the tip of the lower beak is sharp, enlarged towards the base in the oral cavity; the lower beak is reddish-orange and longer than the upper beak. The fin formula of halfbeak from the Seram Sea is D.13-15, P.9-12, V.5-6, A.9-12, C.7/8, and the number of scales at the lateral line is 52-58. Based on molecular identification, the halfbeak species from the Seram Sea was confirmed as Hemiramphus lutkei with a genetic distance of 0.0055 compared to H. lutkei from India, and a genetic distance of 0.0540 with H. balao.

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Section: Introductionmentioning

confidence: 79%

“…Ballyhoo halfbeak is commonly caught in the coastal waters of Rio Grande do Norte Brazil (Oliveira et al 2015). This fish is also an important fish commodity in the Maluku Islands (Ahmad et al 2019;Natan et al 2019), mainly caught using small circular nets (mini purse seines) called "soma roa and giop".…”

Section: Introductionmentioning

confidence: 99%

Record of Hemiramphus lutkei Valenciennes, 1847 from Seram Sea, Maluku, Indonesia with its molecular characterization

Talakua

Suadi²,

Djumanto³

et al. 2022

Biodiversitas

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“…Akbar and Aris (2018) genetic connectivity show the closeness between populations. The low genetic distance value indicates the closeness of the kinship relationship (Achmad et al, 2019). It can be said that the sequence has a low level of diversity.…”

Section: Phylogenetic Tree Analysis and Genetic Distancementioning

confidence: 99%

“…The purpose of this identification is to obtain the genetic quality, characteristics, or character of a species, and to support taxonomic identification, so that it can be used for breeding and conservation purposes (Ragan et al, 1994;Rohani-Ghadikolaei et al, 2011;Djakatara et al, 2018;Annisaqois et al, 2018). Genetic information can be used as a conservation database to determine policy strategies and the existence of a biota (Akbar et al, 2014;Achmad et al, 2019;.…”

Section: Introductionmentioning

confidence: 99%

DNA Barcoding of Red Algae (Rhodophyta) in Ternate Island Sea, North Maluku, Indonesia

Achmad,

Akbar,

Ismail

et al. 2023

JIPK

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Ternate Island is located on the North Maluku Sea. The North Maluku Sea region includes the Wallacea area and the world’s coral triangle. One of the organisms commonly found in this sea is red algae. Study aimed to determine the red algae species by phylogenetic tree analysis based on the rbcL gene as a DNA marker. The preserved red algae tissue samples were extracted with the Geneaid GP100 DNA Extraction Kit Plant. The DNA sample was amplified and then visualized by 1% agarose gel electrophoresis. The amplicon products were sequenced and then aligned with the rbcL gene database that was available at the NCBI gene bank. The phylogenetic tree was constructed using the UPGMA method. The results showed that red algae were identified into four species: namely Gibsmithia hawaiiensis (98.65%), C_rbcL sample was identical to Amansieae sp. (91.50%), D_rbcL sample was identical to Peyssonnelia sp. (95.54%), and G_rbcL sample was similar to Portieria hornemanniI (96.15%). Based on phylogenetic tree analysis, Gibsmithia hawaiiensis from North Maluku is closely related to species from Raja Ampat, West Papua, Indonesia, followed by the Philippines species. Special findings were found to carry out phylogenetic reconstructions that can answer inter-species kinship. The general finding is that the markers used can be used for phylogenetic construction. Phylogenetic construction of Peyssonnelia sp. in North Maluku is related to species from South Africa. North Maluku's Portieria hornemannii is closely related to a species from Korea. Highlight Research This study provides initial information about the DNA barcoding of red algae. Important for management purposes and determining the status of red algae in the future. This scientific information also provides an understanding of the relationships among red algae species in the world's oceans. Global warming has changed the temperature of seawater and affected the population of marine organisms.

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“…The identification of four lineages in mackerel (R. kanagurta) in Banda Aceh and Trang (Andaman Sea) and Can Tho (South China Sea) [29] . Previous studies discussing phylogenetic pelagic fish have been reported such as yellowfin tuna [5,19] , tuna and mackarel in the Indonesian Archipelago [30] in julung-julung fish (Hemirampus sp) [31] . Substitution probability values were found to have similarities in all nucleotide elements (Table 1).…”

Section: Phylogeneticmentioning

confidence: 99%

Phylogenetic and structure genetic of Rastrelliger sp in north Maluku Sea, Indonesia

Akbar,

Wibowo,

Baksir

et al. 2023

Trends Geneti. Evolu.

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Mackerel is a small pelagic fish that has potential value and can be found throughout Indonesian waters. It is feared that high exploration activities will have an impact on the population. Sampling was carried out at the fish landing port and fish auction place (Bacan, Morotai and Ternate). The sample was photographed and the 3 cm swimming fin was taken. The sample is then stored in a tube that has been filled with 96% ethanol solution. The next stage is the process of extraction, amplification, electrophoresis and DNA sequencing. Phylogenetic reconstruction of comparison of primary data (Bacan, Morotai and Ternate) and secondary data (Indian) clarified to form two different populations (clades). Amova population pairwaise (Fst) showed the genetic flow of R. kanagurta population in the waters of the north Maluku Sea (Bacan, Morotai and Ternate). On the other hand, genetic distance shows that populations (Bacan, Morotai and Ternate) were closely related and have strong genetic connectivity.

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DNA barcoding application of garfish (Hemirhampus sp.) in North Maluku Sea

Cited by 3 publications

References 6 publications

Record of Hemiramphus lutkei Valenciennes, 1847 from Seram Sea, Maluku, Indonesia with its molecular characterization

Record of Hemiramphus lutkei Valenciennes, 1847 from Seram Sea, Maluku, Indonesia with its molecular characterization

DNA Barcoding of Red Algae (Rhodophyta) in Ternate Island Sea, North Maluku, Indonesia

Phylogenetic and structure genetic of Rastrelliger sp in north Maluku Sea, Indonesia

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