DNA barcoding as a new tool for food traceability

Galimberti, Andrea; Mattia, Fabrizio De; Losa, Alessia; Bruni, Ilaria; Federici, Silvia; Casiraghi, Maurizio; Martellos, Stefano; Labra, Massimo

doi:10.1016/j.foodres.2012.09.036

Cited by 367 publications

(236 citation statements)

References 140 publications

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“…As reviewed by Galimberti et al (2013) and Madesis, Ganopoulos, Sakaridis, Argiriou, and Tsaftaris (2014), this hypothesis has led to the successful development of DNA-based food authenticity tests. Because DNA is not subject to environmental influence, it directly reflects the species/varietal composition in a food product.…”

Section: Introductionmentioning

confidence: 99%

Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil

Uncu

Frary

et al. 2017

Food Chemistry

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a b s t r a c tThe aim of this study was to compare the performance of a DNA-barcode assay with fatty acid profile analysis to authenticate the botanical origin of olive oil. To achieve this aim, we performed a PCRcapillary electrophoresis (PCR-CE) approach on olive oil: seed oil blends using the plastid trnL (UAA) intron barcode. In parallel to genomic analysis, we subjected the samples to gas chromatography analysis of fatty acid composition. While the PCR-CE assay proved equally efficient as gas chromatography analysis in detecting adulteration with soybean, palm, rapeseed, sunflower, sesame, cottonseed and peanut oils, it was superior to the widely utilized analytical chemistry approach in revealing the adulterant species and detecting small quantities of corn and safflower oils in olive oil. Moreover, the DNA-based test correctly identified all tested olive oil: hazelnut oil blends whereas it was not feasible to detect hazelnut oil adulteration through fatty acid profile analysis. Thus, the present research has shown the feasibility of a PCR-CE barcode assay to detect adulteration in olive oil.

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Section: Introductionmentioning

confidence: 99%

Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil

Uncu

Frary

et al. 2017

Food Chemistry

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“…12S rRNA, 16S rRNA, cytochrome b, and cox1 gene) can be detected in matrices containing a pool of heterogeneous genomic DNA, such as milk (Galimberti et al, 2013;Mafra et al, 2008). Molecular methods may be the solution as the DNA from somatic cells persists even in the ripened cheese (Plath et al, 1997) and it is also possible to extract amplifiable DNA from pasteurized, filtered, and ultrafiltered milk (Bottero et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products

et al. 2016

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A B S T R A C TFor a rapid, specific and sensitive identification of cows', ewes' and goats' milk in mono-species Sicilian dairy products, species-specific duplex-PCR protocol was applied. DNA samples from blood and experimental cheeses of Sicilian autochthonous breeds were extracted to amplify the 12S rRNA (and part of 16S rRNA in case of Ovis aries) mitochondrial species-specific gene fragment. The use of species-specific primers for Bos taurus, Capra hircus and Ovis aries species, after electrophoresis on agarose gel, yielded fragments of 256 bp, 326 bp and 172 bp, respectively. Amplification by duplex-PCR of DNA pools from two species showed detection thresholds of 0.1% of "contaminant" DNA in each mixture. Finally, duplex-PCR assay was applied to experimental cheeses in order to detect the minimum threshold of DNA belonging to one species in cheese made with milk of two species. The results showed a sensitive threshold of 0.1% of ewes' milk in cows' and goats' cheeses, 0.1% of cows' milk in ewes' and goats' cheeses, and finally 0.1% of goats' milk in cows' and ewes' cheeses. The proposed assay represents a rapid and straightforward method of species traceability for the detections of adulteration in Sicilian mono-species dairy products.

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“…The rate of sequence submission has recently intensified for three primary reasons: the numerous and successful DNA barcoding projects [2,3], the advent of Next Generation Sequencing [4] and the subsequent decrease in prices for molecular sequencing services [5].As a consequence, genetic data repositories such as GenBank have been doubling in size every 18 months [6], rising from 606 sequences in the first 1982 release to close to 200 million sequences in the 218 th release in February 2017. Molecular data from more than 260 thousand nominal species is now widely accepted as a paramount source of biological information in all life sciences [7].…”

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Selecting Molecular Markers for a Specific Phylogenetic Problem

Russo¹,

Aguiar²,

Àlex³

et al. 2017

MOJPB

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Submit Manuscript | http://medcraveonline.com of new technologies and the subsequent accessibility of refined methods due to cost reduction contributed to an immeasurable expansion of molecular facilities worldwide [1]. The rate of sequence submission has recently intensified for three primary reasons: the numerous and successful DNA barcoding projects [2,3], the advent of Next Generation Sequencing [4] and the subsequent decrease in prices for molecular sequencing services [5].As a consequence, genetic data repositories such as GenBank have been doubling in size every 18 months [6], rising from 606 sequences in the first 1982 release to close to 200 million sequences in the 218 th release in February 2017. Molecular data from more than 260 thousand nominal species is now widely accepted as a paramount source of biological information in all life sciences [7]. This is an exciting time. Many long existing controversies are in the process of being resolved by an unparalleled amount of data [6,8,9]. Phylogenomics, a dream just a few decades ago, is now changing the face of molecular phylogenetics. It is a revolution second only to the introduction of molecules in the field of phylogenetics in the 1960's.However, the availability of large number of sequences is not necessarily associated with an accurate estimation of phylogenies, due to analytical errors associated with very large sets of sequence data [10][11][12]. Hence, the contentious matter of molecular marker sampling is inhibiting this new breakthrough. Different genes may yield strikingly contradictory topological patterns for a given diversity group [13,14]. Thus, the selection of suitable markers is critical in obtaining accurate estimates, but it is not a straightforward task. In this review, we aim to provide some guidelines for newcomers weighing the suitability of particular molecular markers for a given phylogenetic problem. Homology in Molecular MarkersIn phylogenetic reconstruction, as in comparative biology studies, the single most important concern lies in the matter of homology, a concept that occupies a central position in evolutionary biology [15]. Homology is a qualitative term, defined by equivalence of parts due to inherited common origin [16][17][18].Homology has been more recently defined as the relationship that binds all states of a single character and sets them apart from the states of other characters, supporting the logical equivalence of the notions of homology and synapomorphy (for review see [19]). The comparison of homologous sequences is critical in a phylogenetic analysis, because only homologous characters may reveal the actual phylogenetic pattern.Nevertheless, a number of authors erroneously refer to homology as a synonym for similarity. Molecular biologists are particularly prone to this error, as they assert that 'two sequences share 70% homology' (for reviews on this problem see [15,20]). Two sequences might show 70% similarity, if 7 out of 10 aligned base pairs are identical between them.In molecular sequences, the high...

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DNA barcoding as a new tool for food traceability

Cited by 367 publications

References 140 publications

Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil

Barcode DNA length polymorphisms vs fatty acid profiling for adulteration detection in olive oil

12S rRNA mitochondrial gene as marker to trace Sicilian mono-species dairy products

Selecting Molecular Markers for a Specific Phylogenetic Problem

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