DNA barcoding of invasive species

Cross, Hugh; Lowe, Andrew J.; Gurgel, Carlos Frederico D.

doi:10.1002/9781444329988.ch22

Cited by 10 publications

(7 citation statements)

References 62 publications

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“…Reliable identification of plant material by regulatory/enforcement authorities is a widespread need, including identification of pests, pathogens and invasive species to inform control [ 33 , 34 ], detecting protected species being illegally traded ( figure 1 g , [ 35 , 36 ]), through to identifying food or herbal medicine labelling errors/fraud ( figure 1 h , [ 37 ]).…”

Section: Standard Plant Barcodesmentioning

confidence: 99%

Telling plant species apart with DNA: from barcodes to genomes

Hollingsworth

Bank

et al. 2016

Phil. Trans. R. Soc. B

250

272

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Land plants underpin a multitude of ecosystem functions, support human livelihoods and represent a critically important component of terrestrial biodiversity—yet many tens of thousands of species await discovery, and plant identification remains a substantial challenge, especially where material is juvenile, fragmented or processed. In this opinion article, we tackle two main topics. Firstly, we provide a short summary of the strengths and limitations of plant DNA barcoding for addressing these issues. Secondly, we discuss options for enhancing current plant barcodes, focusing on increasing discriminatory power via either gene capture of nuclear markers or genome skimming. The former has the advantage of establishing a defined set of target loci maximizing efficiency of sequencing effort, data storage and analysis. The challenge is developing a probe set for large numbers of nuclear markers that works over sufficient phylogenetic breadth. Genome skimming has the advantage of using existing protocols and being backward compatible with existing barcodes; and the depth of sequence coverage can be increased as sequencing costs fall. Its non-targeted nature does, however, present a major informatics challenge for upscaling to large sample sets.This article is part of the themed issue ‘From DNA barcodes to biomes’.

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Section: Standard Plant Barcodesmentioning

confidence: 99%

Telling plant species apart with DNA: from barcodes to genomes

Hollingsworth

Bank

et al. 2016

Phil. Trans. R. Soc. B

250

272

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“…Although DNA barcoding offers exciting new research possibilities ( Cross et al . 2011 ), it is no panacea.…”

Section: Conclusion: the Way Forwardmentioning

confidence: 99%

Hitting the right target: taxonomic challenges for, and of, plant invasions

et al. 2013

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Taxonomic resources are essential for the effective management of invasive plants because biosecurity strategies, legislation dealing with invasive species, quarantine, weed surveillance and monitoring all depend on accurate and rapid identification of non-native taxa, and incorrect identifications can impede ecological studies. On the other hand, biological invasions have provided important tests of basic theories about species concepts. Modern taxonomy therefore needs to integrate both classical and new concepts and approaches to improve the accuracy of species identification and further refine taxonomic classification at the level of populations and genotypes in the field and laboratory.

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“…Environmental conditions differ to a greater extent between populations than between individuals in separate populations. Higher differences between populations according to the amount of polymorphic DNA loci might reflect bigger differences in site features [15][16][17][18]. For I. glandulifera, studied by us in parallel, genetic differentiation between populations was much smaller (Φ PT =0.51) [32].…”

Section: Ignssr101 -Ef025990) or Longer (Ignssr240 -Ef025997mentioning

confidence: 99%

“…Reviewed by Cross et al [17] and Dormontt et al [18], populations of various alien species might be distinguished by amplified fragment length polymorphism (AFLP) [19][20][21], by random amplified polymorphic DNA (RAPD) [22][23][24], by microsatellite/simple sequence repeat (SSR) [25][26][27][28][29] and by inter simple sequence repeat (ISSR) [24,30] loci. Among Balsaminaceae, the biggest variety of molecular markers, including nuclear microsatellites [25,31], chloroplast microsatellites [25] and RAPD [32] were applied to Impatiens glandulifera.…”

Section: Introductionmentioning

confidence: 99%

Molecular diversity of small balsam populations in relation to site characteristics

et al. 2013

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Climatic shifts within recent decades created favorable conditions for invasive species flourishing in more Northern parts of Europe. Our study was aimed at evaluation of genetic variability and habitat features of Impatiens parviflora populations growing in Lithuania. Twenty one populations were selected and analysed using simple sequence repeat (SSR) and randomly amplified polymorphic DNA (RAPD) assays. Evaluated by SSRs, 315 individuals were all monomorphic and homozygous at 4 loci and heterozygous at 1 locus. RAPD analyses revealed that the percentage of polymorphic DNA loci (% P) per population ranged from 7 to 39% and genetic differentiation between populations was ΦPT=0.790 (P<0.01). Genetic distances among populations (0.135–0.426) correlated significantly with geographical distances (r=0.183; P<0.008). Populations in overmoistured soil contained higher % P (28.3) when compared to drier soil (18.7; P<0.05). All recorded populations were close to roads; their % P did not depend on proximity to buildings, light intensity or population size. Our RAPD analyses indicate multiple introductions of this species in Lithuania. Analyses of I. parviflora at SSR and RAPD loci show that the invasion process is reflected in genetic structure.

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DNA barcoding of invasive species

Cited by 10 publications

References 62 publications

Telling plant species apart with DNA: from barcodes to genomes

Telling plant species apart with DNA: from barcodes to genomes

Hitting the right target: taxonomic challenges for, and of, plant invasions

Molecular diversity of small balsam populations in relation to site characteristics

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