DNA barcoding of snakeskin gourami Trichogaster pectoralis and blue bourami Trichogaster trichopterus based on cythocrome c oxidase subunit I (COI) gene

Abstract

Snakeskin gourami Trichogaster pectoralis and blue gourami Trichogaster trichopterus are the species from the genus of Trichogaster. There are three species of the genus Trichogaster in Indonesia. This research aimed to identify the sequence of cytochrome c oxidase subunit I (COI) gene of mtDNA, and construct the phylogenetic trees among species. The stage of research consisted of DNA isolation, DNA amplification using PCR (Polymerase Chain Reaction) and sequencing COI gene in mtDNA region. Sequencing of the COI gene produced 604 bp for snakeskin gourami and 628bp for blue gourami. The result of BLASTn (Basic Local Aligment Search Tool-nucleotide) analysis showed that snakeskin gourami from Kelekar River had 100% similarity of snakeskin from Indonesia and Philippines and farthest (88%) with snakeskin gourami from France. Blue gourami from Inderalaya had 100% similarity with three spot gourami from Australia, South Africa and India and farthest (88%) with snakeskin gourami from Japan. The phylogenetic tree indicated that snakeskin and blue gourami were clustered in separate branches, where snakeskin gourami had 100% similarity to the same species from Indonesia and the Philippines, and blue gourami had 100% identity to the same species from Japan.

Markers of Genetic Variation in Blue Gourami (Trichogaster trichopterus) as a Model for Labyrinth Fish

“…Prior to these studies, C. damascina was not a well-defined species. Likewise, the COI gene is widely used in assessing genetic variation in both geographical distribution and aquaculture contexts [ 51 , 52 ]. This gene is suitable for the separation of different species belonging to the genus Trichogaster, but not of the order Anabantiformes ( Figure 23 and Figure 24 ).…”

Study Identification of Some Species of Fish Using the Partial Fragment of Mitochondrial Cytochrome Oxidase Subunit-1Gene (COI) in Danau Panggang, South Borneo

“…The COI gene sequence produced in the T. pectoralis sample in the flooded swamps of Danau Panggang ranges from 510 bp-585 bp. Research by Syaifudin et al (2019) states that the resulting T. pectoralis COI gene sequence is at 604 bp and has 100% similarity to the same species in Indonesia and the Philippines. Gene sequencing (DNA sequencing) for phylogenetic analysis is rarely performed because the cloning of homologous genes from several samples still needs high cost (Kocher and Stepien, 1997).…”

DNA Authentication of Indonesian Leaffish <i>Pristolepis grooti</i> from Kelekar River and Ogan River in South Sumatra Based on <i>Cytochrome C Oxidase Subunit I (COI)</i> Gene

“…This molecular technique can be used for determining genetic analysis systematically and at the taxonomic level of species, populations and also plays a role in the selection process of fish genetic diversity. The application of DNA barcoding has been conducted on several aquatic organisms i.e Hemibagrus nemurus (Syaifudin et al 2017), flatfish from Vietnam (Truong et al 2020), snake-skin gourami, blue gourami (Syaifudin et al 2019), Pristolepis fasciata from Malaysia (Noikotr 2019) striped snakehead, catfish Mystus singaringan (Pramono et al 2019), and ocellated snakehead (Syaifudin et al 2020). In this study, the application of DNA barcodes has an important role in determining COI gene sequences as a taxonomic tool to reveal the genetics authentication of species among Indonesian leaffish from the Kelekar and the Ogan River in South Sumatra.…”