The featherback Chitala sp in Indonesia inhabit riverine of Sumatra, Java and Kalimantan. Featherbacks have been protected as threatened species. This research aims to identify the sequence of Cytochrome C Oxidase Subunit I (COI) gene of mtDNA, and construct the phylogenetic trees between species of featherbacks in South Sumatra. This research was conducted in March–August 2020. The methods used in barcoding species and determining phylogenetic i.e., DNA isolation, DNA amplification using PCR and sequencing of COI gene regions of mtDNA. The COI gene was sequenced by PCR (Polymerase Chain Reaction) with optimum annealing temperature 500C for 30s with 35 cycles. After editting, sequence length of the COI gene of featherbacks was 621 base pairs (bp) nucleotide. Based on BLASTanalysis, specimen CLP2 from PT PLN Indralaya had the highest similarity (99.28%) to C. lopis (Malaysia), then 98.88% to the same species from Kampar River, Riau. Speciemens from Musi River (CLS1, CLS 3) and PT PLN (CLP3) indicated the highest similarity of 95.19% with C. chitala from India. The phylogenetic trees showed that Chitala formed four sub-clusters and it was clearly distant between species C. lopis and C. chitala (bootstrap value = 73).

Snakeskin gourami Trichogaster pectoralis and blue gourami Trichogaster trichopterus are the species from the genus of Trichogaster. There are three species of the genus Trichogaster in Indonesia. This research aimed to identify the sequence of cytochrome c oxidase subunit I (COI) gene of mtDNA, and construct the phylogenetic trees among species. The stage of research consisted of DNA isolation, DNA amplification using PCR (Polymerase Chain Reaction) and sequencing COI gene in mtDNA region. Sequencing of the COI gene produced 604 bp for snakeskin gourami and 628bp for blue gourami. The result of BLASTn (Basic Local Aligment Search Tool-nucleotide) analysis showed that snakeskin gourami from Kelekar River had 100% similarity of snakeskin from Indonesia and Philippines and farthest (88%) with snakeskin gourami from France. Blue gourami from Inderalaya had 100% similarity with three spot gourami from Australia, South Africa and India and farthest (88%) with snakeskin gourami from Japan. The phylogenetic tree indicated that snakeskin and blue gourami were clustered in separate branches, where snakeskin gourami had 100% similarity to the same species from Indonesia and the Philippines, and blue gourami had 100% identity to the same species from Japan.

The feed is one of the components that support the growth and survival in fish culture. One of the alternative feed ingredients which could use as other feed ingredient was Indigofera zollingeriana leaves. The aims of this research was to determine the use of Indigofera zollingeriana leaves on the growth performance of Patin (Pangasius sp.). This research consisted of five treatments and three replications. Indigofera zollingeriana leaves were used in the diet amount of P0 (0%) (without Indigofera zollingeriana leaves addition), P1 (10% Indigofera zollingeriana leaves addition), P2 (20% Indigofera zollingeriana leaves addition), P3 (30% Indigofera zollingeriana leaves addition), and P4 (40% Indigofera zollingeriana leaves addition). The result showed that the use of Indigofera zollingeriana leaves had a significant effect on the fish growth performance. The use of 20% zollingeriana Indigofera produced the best results of the absolute weight of 2.15 g, 1.00% daily growth rate and feed efficiency of 44.60%.

Bahan kimia yang digunakan sebagai bahan anastesi ikan dan senyawa antimikroba sudah harus diminimalisir untuk keamanan pangan. Bahan kimia dapat membawa dampak negatif karena dapat terakumulasi dalam tubuh ikan dan membawa pengaruh buruk terhadap kesehatan manusia yang mengkonsumsinya. Pendekatan yang dapat dilakukan  untuk mengatasi hal tersebut dengan penggunaan bahan alami yaitu ekstrak akar tuba. Tujuan dari penelitian ini untuk mengetahui pengaruh berbagai konsentrasi ekstrak akar tuba terhadap status kesehatan ikan nila. Penelitian  ini digunakan Rancangan Acak Lengkap (RAL) dimana digunakan 5 perlakuan pada uji toksisitas letal dan 6 perlakuan pada uji toksisitas subletal dengan masing-masing perlakuan dilakukan  3 kali ulangan. Konsentrasi perlakuan ekstrak akar tuba yang digunakan pada pengujian toksisitas letal adalah 0 ml/l, 0,001 ml/l, 0,002 ml/l, 0,003 ml/l, 0,004 ml/l. Hasil pengujian toksisitas letal digunakan pada pengujian toksisitas subletal untuk mendapatkan nilai LC50 96 Jam. Hasil pengujian darah ikan nila pada uji toksisitas letal berturut-turut yaitu  jumlah total eritrosit PO : 11,3 x 105 sel/mm3, P1 : 2,6 x 105 sel/mm3, P3 : 2,2 x 105 sel/mm3, P4 : 1,7 x 105 sel/mm3, P5 :1,9 x 105 sel/mm3. Kadar Hematokrit PO : 25%,  P1 : 23%, P2 : 19 %, P3 : 15%, P4 :17%. Kadar Hemoglobin PO : 10,13 g/dl,  P1 : 7,7 g/dl,  P2 : 6,4 g/dl,  P3 : 5,0 g/dl, P4 : 5,7 g/dl . Hasil pengujian pada uji toksisitas subletal menunjukkan angka mortalitas ikan nila yaitu PO : 0%,  P1 : 6,27%,  P2 : 13,77%,  P3 : 14,17%, P4 : 37,10%, P5 : 77,10%. Hasil Pengujian toksisitas subletal menunjukkan  bahwa  LC50-96 jam ekstrak akar tuba  diperoleh nilai sebesar 0,003 ml/ L.

Biofloc Technology (BFT) menjadi salah satu teknologi alternatif dalam akuakultur yang bertujuan untuk memperbaiki kualitas air dan meningkatkan efesiensi pemanfaatan nutrisi. Tujuan dari Pengabdian Kepada Masyarakat ini adalah untuk memperkenalkan dan memberikan informasi kepada masyarakat dan petani tentang budidaya ikan Lele dengan sistem bioflok. Kegiatan ini dilakukan di Desa Pandan Arang, Kabupaten Ogan Ilir dengan menggunakan metode pemberdayaan partisipati aktif kelompok pembudidaya ikan pada mitra yang diajak kerjasama dengan transfer ilmu dan teknologi, meliput beberapa tahapan yaitu: 1) Persiapan; 2) Penyuluhan pembuatan pakan dan budidaya ikan; 3) Pembuatan demplot dan pendampingan; 4) Evaluasi dan pembuatan laporan hasil kegiatan. Hasil yang didapat bahwa kegiatan budidaya ikan lele dengan menerapkan sistem bioflok memberikan hasil dapat memperbaiki kualitas air pada budidaya ikan lele, terutama dalam menurunkan nilai ammonia dalam media budidaya. Serta memberikan pertumbuhan optimal pada ikan lele dengan bobot mutlak 8,4 gram/ekor dan pertumbuhan panjang mutlak 8,6 cm/ekor. Kata kunci: Biofloc Technology, Ikan Lele, Desa Pandan Arang, Budidaya Ikan ABSTRACT Biofloc Technology (BFT) is an alternative technology in aquaculture that aims to improve water quality and increase the efficiency of nutrient utilization. The purpose of this social dedication are to introduce and provide information to the public and farmers with biofloc system. This activity is carried out in Pandan Arang Village, Ogan Ilir District with an active participatory empowerment method for farmers fish in partners who are invited to collaborate with the transfer of knowledge and technology, covering several stages, 1) preparation; 2) counseling for feed and fish cultivation; creating demonstration plots and assistance; 4) evaluation and making reports on the results of activities. The results showed that catfish culture using the biofloc technology could improve water quality in catfish aquaculture, particularly in reducing the value of ammonia in the culture medium. As well as providing optimal growth in catfish witn an absolute weight of 8,4 gr and absolute length growth of 8,6 cm. Keywords: Biofloc Technology, Catfish, Pandan Arang Village, Aquaculture