DNA barcoding of some medicinally important plant species of Lamiaceae family in India

Thakur, Vishwa Vijay; Tripathi, Niraj; Tiwari, Sharad

doi:10.1007/s11033-021-06356-3

Cited by 12 publications

(5 citation statements)

References 46 publications

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“…DNA barcoding can help the administrative specialists to devise an instrument for quality control and can to a great extent support the home grown drug enterprises (Zahra et al, 2016). Thakur et al, (2021) shows the results represent that among all the DNA barcoding loci, rbcL locus can precisely and productively recognize every one of the concentrated on species followed by trnH-psbA and trnL-F to reestablish the dissolved purchaser certainty. Anbazhagan et al, (2014) performed a study where in young leaves of the plant were collected and genomic DNA was isolated from the leaves using CTAB extraction method.…”

Section: Discussionmentioning

confidence: 82%

Pollen Morphological and Molecular identification, and deposition of Ocimum species

Alex

Senthilkumar

2022

Preprint

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Ocimum species is an exceptionally exchanged and broadly utilized species of Ayurvedic medication inferable from its colossal helpful potential, which has prompted defilement of the two definitions as well as exchanged crude medications. To fulfill the guideline worldwide market necessities, fast and proficient strategies for confirmation are required which can stay up with the rising interest in the spice. In 2019, four Ocimum species were collected from the Tiruchirappalli districts and identified based on morphological and molecular levels. Pollen morphological studies were carried out using Scanning electron Microscopy. The shape of the studied, plants are prolate, oblate-spherical and percolate Morphological characters are identified based on the physical characters tod the herbarium sheet was submitted in St Joseph’s College. Molecular identification is carried out by PCR with help of three primers rbcL, trnH-psbA, and trnL-F. The conserved region is identified and constructed in a phylogenetic tree based on interspecific and intraspecific molecular markers with help of The Molecular Evolutionary Genetics Analysis (MEGA) software. Finally, sequences are submitted to NCBI and got an accession number from Genbank.

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Section: Discussionmentioning

confidence: 82%

Pollen Morphological and Molecular identification, and deposition of Ocimum species

Alex

Senthilkumar

2022

Preprint

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“…Therefore, it is necessary to establish a rapid, inexpensive, efficient, and accurate method by which to identify chrysanthemum ‘Daboju’ and distinguish it from other cultivars or from adulterated preparations. In recent years, DNA barcoding has gained popularity in species identification [ 48 , 49 , 50 , 51 ]. Its advantage is that the target sequence comes from a known region of the genome, which helps in evolutionary studies.…”

Section: Discussionmentioning

confidence: 99%

Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Cai

Gao

Zhang

et al. 2022

Plants

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Chrysanthemummorifolium Ramat. ‘Daboju’ is a C. morifolium cultivar with important ornamental and medicinal values, and is often used in the treatment of colds, blurred vision, dizziness, and itchy skin. As the morphological characteristics of C. morifolium ‘Daboju’ are very similar to those of other C. morifolium cultivars, they are often confused in practice. However, the medicinal value and practical use of C. morifolium depends on using the correct rapid and accurate identification of C. morifolium ‘Daboju’ and its differentiation from other, morphologically similar C. × morifolium cultivars. Twenty-one polymorphic start codon-targeted (SCoT) primers were amplified in 21 distinct C. morifolium cultivars. One cultivar-specific DNA marker was developed with the aim of the rapid and accurate identification of C. morifolium ‘Daboju’ and its differentiation from other, similar C. morifolium cultivars. Twenty-one polymorphic start codon-targeted (SCoT) primers were amplified in 21 distinct C. morifolium cultivars. One cultivar-specific 385-bp amplicon (named SCoT36-385), amplified only in C. morifolium ‘Daboju’ (and in all samples of this cultivar), was identified, cloned, and sequenced. Subsequently, a sequence-characterized amplified region (SCAR) marker (named DBJF/DBJR), generating a 360-bp amplicon, was developed from SCoT36-385 and tested for amplification in all 21 C. morifolium cultivars, ten C. morifolium ‘Daboju’ populations, and different simulated adulterations of ‘Daboju’ with other cultivars. The primers amplified the specific 360-bp-long DNA fragment in all the tested C. morifolium ‘Daboju’ samples but failed in the absence of ‘Daboju’. The detection limit of the SCAR primer pair (DBJF/DBJR) was 100 pg of DNA extracted from C. morifolium ‘Daboju’. Hence, this SCAR marker has a very high detection sensitivity, and can be used for accurate and rapid identification of C. morifolium ‘Daboju’. It can play an important role in ensuring the quality of medicinal preparations and protecting C. morifolium ‘Daboju’ germplasm resources in breeding programs and in identifying lines generated from this cultivar.

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“…Chen et al (2010) in a series of publications, reported that the efficiency of ITS2 and trnH-psbA markers aided easy identification of medicinal and herbal materials (Chen et al, 2013. Since then, many studies have examined several gene regions which helped in the identification of species, including accD (He et al, 2014a;Mao et al, 2014), atpF-atpH (Ran et al, 2010;Zuo et al, 2011;Thakur et al, 2019Thakur et al, , 2021, rpoB (Zuo et al, 2011;Singh et al, 2012;Liu et al, 2016), ndhJ (He et al, 2014a;2014b), ycf1 and ycf5 (Luo et al, 2010;Dong et al, 2015), rpoC1 (Luo et al, 2010;Shi et al, 2011;Khan et al, 2012;Singh et al, 2012;Aziz et al, 2015;Naim and Mahboob, 2020).…”

Section: Single-locus Dna Barcode Markersmentioning

confidence: 99%

Advancements and future prospective of DNA barcodes in the herbal drug industry

Mahima

Kumar²,

Rakhesh³

et al. 2022

Front. Pharmacol.

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Ethnopharmacological relevance: The past couple of decades have witnessed the global resurgence of medicinal plants in the field of herbal-based health care. Increased consumption of medicinal plants and their derivative products is the major cause of the adulteration issues in herbal industries. As a result, the quality of herbal products is affected by spurious and unauthorized raw materials. Recent development in molecular plant identification using DNA barcodes has become a robust methodology to identify and authenticate the adulterants in herbal samples. Hence, rapid and accurate identification of medicinal plants is the key to success for the herbal industry. Aim of the study: This paper provides a comprehensive review of the application of DNA barcoding and advanced technologies that have emerged over the past 10 years related to medicinal plant identification and authentication and the future prospects of this technology.Materials and methods: Information on DNA barcodes was compiled from scientific databases (Google Scholar, Web of Science, SciFinder and PubMed). Additional information was obtained from books, Ph.D. thesis and MSc. Dissertations.Results: Working out an appropriate DNA barcode for plants is challenging; the single locus-based DNA barcodes (rbcL, ITS, ITS2, matK, rpoB, rpoC, trnH-psbA) to multi-locus DNA barcodes have become the successful species-level identification among herbal plants. Additionally, multi-loci have become efficient in the authentication of herbal products. Emerging advances in DNA barcoding and related technologies such as next-generation sequencing, high-resolution melting curve analysis, meta barcodes and mini barcodes have paved the way for successful herbal plant/samples identification.Conclusion: DNA barcoding needs to be employed together with other techniques to check and rationally and effectively quality control the herbal drugs. It is suggested that DNA barcoding techniques combined with metabolomics, transcriptomics, and proteomics could authenticate the herbal products. The invention of simple, cost-effective and improved DNA barcoding techniques to identify herbal drugs and their associated products of medicinal value in a fool-proof manner will be the future thrust of Pharmacopoeial monograph development for herbal drugs.

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DNA barcoding of some medicinally important plant species of Lamiaceae family in India

Cited by 12 publications

References 46 publications

Pollen Morphological and Molecular identification, and deposition of Ocimum species

Pollen Morphological and Molecular identification, and deposition of Ocimum species

Development and Application of a Cultivar-Specific Sequence-Characterized Amplified Region (SCAR) Marker for the Detection of Chrysanthemum morifolium Ramat. ‘Daboju’

Advancements and future prospective of DNA barcodes in the herbal drug industry

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