DNA Base Composition and Taxonomy of Some Micrococci

Boháček, J.; Kocur, M.; Martinec, T.

doi:10.1099/00221287-46-3-369

Cited by 75 publications

(24 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The serological examination Q f the 10 flagellated strains 1:hus substantiates 'the conclusion drawn by BohBCek et al (2)(3)(4) and Kocur et al (9) that these strains do not belong to the genus Micro co ccus.…”

Section: Discussionsupporting

confidence: 55%

Serological Investigations of Planococcus Strains

Oeding

1971

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

BohBCek et al. have proposed that the flagellated, gram-positive cocci be included in the genus PZanococcus Migula, particularly on the basis of their per cent guanine plus cytosine content. A serological examination of these organisms (10 strains) by the author revealed no antigenic relationship to staphylococci or micrococci, thus substantiating the conclusion of BohaCek et al. that they do not belong to the genus Micrococcus.Antigenically , the group of strains was rather heterogeneous. Most authors have placed the flagellated, The double diffusion in agar technique was the gram-positive cocci of marine origin in the same as that used by Haukenes and Oeding (7). Thick genus Micrococcus Cohn. BohACek et ale (2-4) suspensions Of live 18-hr cultures were tested against and Kocur et al. (9) demonstrated that these muted immune sera. Polysaccharide A (6), polycoccus aureus, polysaccharide B from S. epidermidis, cytosine (GC) content and suggested that included as references. they be in the genus Pzanococcus Cell walls were prepared from the two flagellated Migula, 1894. Schleifer and Kandler ( 13) found strains 3 16 and 1849. that the 10 strains studied by BohaCek et al. and Kocur et al. were uniform with respect to bacteria differ considerably from micrococci and staphylococci in their per cent guanine plus saccharide 263 (81, and Protein A (5 I from Staphyloand polysacchaide AC from ~j c r o c o c c u s (11) wereRESULTS the type of inurein present in their cell walls and that this murein was different from the mureins found in the cell walls of members of the genera Micrococcus and Staphylococcus. Their results thus supported the taxonomic suggestion of BohiCek et al. (2, 3).The purpose of the study reported here was to examine serologically the 10 strains of flagellated cocci used by the above-mentioned authors to determine (i) whether they are serologically related to staphylococci and micrococci and (ii) what serological similarities or dissimilarities exist within the group. MATERIALS AND METHODS Bacterial strains. The 10 strains of flagellated, marine cocci were kindly furnished by M. Kocur, Czechoslovak Collection of Microorganisms (CCM), Brno. Their cu.ltural, morphological, and biochemical characteristics have been described by BohiCek et al. (9).Sera. Preirnmune sera and immune sera were obtained from the Gade Institute's breed of New Zealand White rabbits. Immune sera were produced by intravenous injections of formalin-killed bacteria (1 2).Methods. Agglutination was carried out on slides by using live bacteria (12) grown on nutrient agar at 30 C for 18 hr.

show abstract

Section: Discussionsupporting

confidence: 55%

Serological Investigations of Planococcus Strains

Oeding

1971

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

show abstract

“…Many authors continued to place motile cocci in the genus Micrococcus , arguing that motility was not sufficient grounds to place the organisms in a completely different genus. However, it was demonstrated subsequently that the mol% G + C content of micrococci covered a large range (Bohácêk et al . 1967 1968), and that those with a mol % G + C content in the range 40–50% should be considered as members of the genus Planococcus (Bohácêk et al .…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a novel hydrocarbon-degrading, Gram-positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. nov.

Engelhardt¹,

Daly²,

Swannell³

et al. 2001

J Appl Microbiol

121

View full text Add to dashboard Cite

Aims: Characterization of a bacterial isolate (strain MAE2) from intertidal beach sediment capable of degrading linear and branched alkanes. Methods and Results: A Gram-positive, aerobic, heterotrophic bacterium (strain MAE2), that was capable of extensive degradation of alkanes in crude oil but had a limited capacity for the utilization of other organic compounds, was isolated from intertidal beach sediment. MAE2 had an obligate requirement for NaCl but could not tolerate high salt concentrations. It was capable of degrading branched and n-alkanes in crude oil from C11 to C33, but was unable to degrade aromatic hydrocarbons. Comparative 16S rRNA sequence analysis placed the isolate with members of the genus Planococcus. That ®nding was corroborated by chemotaxonomic and physiological data. The fatty acid composition of strain MAE2 was very similar to the type species of the genus Planococcus, P. citreus (NCIMB 1493 T ) and P. kocurii (NCIMB 629 T ), and was dominated by branched acids, mainly a15:0. However, the 16S rRNA of strain MAE2 had less than 97% sequence identity with the type strains of P. citreus (NCIMB 1439 T ), P. kocurii (NCIMB 629 T ) and two Planococcus spp. (strain MB6-16 and strain ICO24) isolated from Antarctic sea ice. This indicated that strain MAE2 represented a separate species from these planococci. Morphologically, the isolate resembled P. okeanokoites (NCIMB 561 T ) and P. mcmeekinii S23F2 (ATCC 700539 T ). The cellular fatty acid composition of P. okeanokoites and P. mcmeekinii was considerably different from strain MAE2, and the mol % G + C content of P. mcmeekinii was far lower than that of MAE2. Conclusions: On the basis of phenotypic and genotypic data, it is proposed that strain MAE2 is a new species of Planococcus, Planococcus alkanoclasticus sp. nov., for which the type strain is P. alkanoclasticus MAE2 (NCIMB 13489 T ). Signi®cance and Impact of the Study: Planococcus species are abundant members of the bacterial community in a variety of marine environments, including some in sensitive Antarctic ecosystems. The occurrence of hydrocarbon-degrading Planococcus spp. is potentially of importance in controlling the impact of hydrocarbon contamination in sensitive marine environments.

show abstract

“…Prototrophy with respect to adenine, L-tryptophan, and L-histidine is uncertain in these strains. Bohacek, Kocur & Martinec, 1967;Jeffries et al, 1968;Rosypal et al 1966). Transformation was not expected by strains of Staphylococcus aureus or St. epidermidis either, as members of the genus Staphylococcus as recommended by Evans et al (1955) are facultative anaerobes and differ in numerous characteristics, including a very low GC ratio (32 to 35%) (Auletta & Kennedy, 1966;Baird-Parker, 1965 …”

Section: Nutritional Characterization Of Donor Strainsmentioning

confidence: 99%