DNA-Based Genetic Markers for Rapid Cycling Brassica Rapa (Fast Plants Type) Designed for the Teaching Laboratory

Abstract

We have developed DNA-based genetic markers for rapid cycling Brassica rapa (RCBr), also known as Fast Plants. Although markers for B. rapa already exist, ours were intentionally designed for use in a teaching laboratory environment. The qualities we selected for were robust amplification in PCR, polymorphism in RCBr strains, and alleles that can be easily resolved in simple agarose slab gels. We have developed two single nucleotide polymorphism (SNP) based markers and 14 variable number tandem repeat (VNTR)-type markers spread over four chromosomes. The DNA sequences of these markers represent variation in a wide range of genomic features. Among the VNTR-type markers, there are examples of variation in a non-genic region, variation within an intron, and variation in the coding sequence of a gene. Among the SNP-based markers there are examples of polymorphism in intronic DNA and synonymous substitution in a coding sequence. Thus these markers can serve laboratory exercises in both transmission genetics and molecular biology.

The Gene Encoding Dihydroflavonol 4-Reductase Is a Candidate for the anthocyaninless Locus of Rapid Cycling Brassica rapa (Fast Plants Type)

“…The rapid cycling Brassica rapa (Fast Plants type) strains Purple Stem, Hairy ( ANL / ANL ) and Non-Purple Stem, Yellow-Green Leaf ( anl / anl ) were obtained from Carolina Biological Supply (Burlington, NC). Purple ( ANL / ANL ) strain DWRCBr76 was reported previously [ 10 ]. Non-purple ( anl / anl ) strain DWRCBr57 was derived from DWRCBr53 [ 10 ] with selection for homozygosity of DNA markers on chromosome A09.…”

“…Purple ( ANL / ANL ) strain DWRCBr76 was reported previously [ 10 ]. Non-purple ( anl / anl ) strain DWRCBr57 was derived from DWRCBr53 [ 10 ] with selection for homozygosity of DNA markers on chromosome A09.…”

“… 1 Position of 5’ end of forward primer in Brassica rapa FPsc v1.3, DOE-JGI, http://phytozome.jgi.doe.gov/ [ 12 ]. 2 Source Slankster et al [ 10 ] 3 Developed by Kresovich and applied to RCBr as reported by Slankster et al [ 10 ] D9BrapaS5 and D9BrapaS12 are dominant markers which give a PCR product in DWRCBr76 but not in DWRCBr57. …”

Effects of Phosphorus on In-vitro Seed Germination and Seedling Growth of Brassica rapa L. in Arsenic Condition

“…Wild forms of the hot turnip and its relatives are found over west Asia and Europe, suggesting their domestication took place somewhere in that area (Susan, 2010;Daniel et al, 2012;Rdnld, 2018). Small, tender varieties of this crop are grown for human consumption, while larger varieties are grown as feed for livestock (Vogl-Lukasser et al, 2007;Slankster et al, 2012). Boiled turnip is 94% water, 5% carbohydrates, and 1% protein, with negligible fat.…”

“…Boiled turnip is 94% water, 5% carbohydrates, and 1% protein, with negligible fat. It also contains iron, calcium, potassium, sodium, and significant amount of vitamin A, B, C and K. It is grown in well drained loam and sandy soils, and needs constant irrigation during the growing season (Susan, 2010;Slankster et al, 2012;Rdnld, 2018).…”