DNA‐Based Methods

Evaluation of a Rapid PCR-Based Method for Species Identification of Raw and Processed Fish and Shrimps

“…The amount of muscle tissue should not be <10 mg, as fish fillet has a high water content and low concentration of DNA, especially in white muscle (Rehbein, 2009). We found 10−20 mg of muscle tissue suitable to release enough DNA for PCR of mitochondrial gene segments.…”

“…For many years, RFLP analysis was the method of choice for identification of fish and shrimp species (Rehbein, 2009). In many applications it was not necessary to purify the PCR products before being digested by restriction endonucleases.…”

The current status of DNA barcoding technology for species identification in fish value chains

“…Though various DNA techniques have been used to identify intentional cases of market substitution and misbranding (Bossier, 1999;Martinez et al, 2005;Wong and Hanner, 2008;Rasmussen and Morrissey, 2008;Cohen et al, 2009;Rehbein, 2009b;Miller and Mariani, 2010;Handy et al, 2011), DNA barcoding is seen as an innovation in authentication techniques because of its breadth of application and its reliance on an expert-identified reference library (see Herbert et al, 2003;Hanner et al, 2011;Handy et al, 2011). Compared to existing methods, DNA barcoding is argued to be superior because of the uniformity of data collection and organization methods in barcode databases.…”