The current status of DNA barcoding technology for species identification in fish value chains

Clark, Lisa F.

doi:10.1016/j.foodpol.2015.05.005

Cited by 51 publications

(21 citation statements)

References 25 publications

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“…Probes or scanners that analyse genetic markers, proteins, chemicals or hormones to determine the species and sex of the catch (Angers et al, ; Ashoor & Knox, ; Devlin & Nagahama, ; Hyde et al, ; Knuutinen & Harjula, ; Liu et al, ) could be developed and integrated into EM systems. Chromatography, various DNA‐based analyses and protein‐based analyses can be used to identify fish species (Angers et al, ; Ashoor & Knox, ; Clark, ; Galimberti et al, ; Knuutinen &Harjula, 1998). Depending on the species‐specific sex determination system, analyses of hormones, proteins and genes could be used to determine the sex of the catch (Devlin & Nagahama, ; Liu et al, ).…”

Section: Results: Methods To Expand Data Fields and Improve The Accurmentioning

confidence: 99%

Increasing the functionalities and accuracy of fisheries electronic monitoring systems

Gilman

Legorburu

Fedoruk³

et al. 2019

Aquatic Conservation

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1. Fisheries-dependent data underpin essential scientific and management applications. Electronic monitoring (EM) systems are increasingly being used to supplement human observer programmes and provide coverage where none previously existed.2. Candidate methods were identified to expand EM functionalities to collect data fields of human observer programmes that contemporary EM systems either cannot collect or require improved accuracy. Options were also identified to enable EM systems to collect new data fields that human observers cannot collect, prioritized by scientists, managers and the catch sector. 3. Many EM limitations could be resolved through simple changes of repositioning existing or adding new cameras with suitable fields of view and resolution, integrating additional sensors, and making minor modifications to fishers' practices. Research, development and trials, however, are required for possible EM integration of some existing and emerging technologies. 4. Whether an EM improvement method should be pursued requires consideration of: the relative importance for meeting monitoring objectives; the accuracy and cost of alternative monitoring methods, such as data collection by fishers with EM auditing, and by dockside inspections; impacts on fishing operations and crew safety; and net costs.5. Active support from fishers is necessary for EM collection of some data fields, where EM enables auditing fisher compliance with required procedures. Having EM systems supply data desired by the seafood industry could augment their support for EM and incentivize fisher cooperation.6. EM systems have the capacity to collect most data fields collected by human observer programmes with high precision and in some cases improved accuracy, to supply information of interest to seafood companies, and to meet expanding data requirements as fisheries management frameworks continue to transition to implementing elements of ecosystem-based fisheries management.

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Section: Results: Methods To Expand Data Fields and Improve The Accurmentioning

confidence: 99%

Increasing the functionalities and accuracy of fisheries electronic monitoring systems

Gilman

Legorburu

Fedoruk³

et al. 2019

Aquatic Conservation

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“…Though COI gene is widely used as a genetic marker for fish species authentication, it is not an appropriate marker for all aspects of species identification (Clark 2015). The COI gene sequences only could not determine a hybrid because mtDNA exhibits maternal inheritance.…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnostic markers of Tachysurus fulvidraco and Leiocassis longirostris and their hybrids

et al. 2016

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BackgroundBagridae is an important family of catfishes and has a high market demand. Recently, more cultivable Bagridae fishes are being exploited in China, and hybridization of some species has been carried out to achieve better growth performance, favorable sex ratios and better disease resistance. Yet, these hybrids have further increased the difficulties of taxonomy identification due to morphological indistinguishableness.ResultsIn this study, the molecular identification technologies for Tachysurus fulvidraco, Leiocassis longirostris sand their hybrids were successfully established by using mitochondrial COI and nuclear ITS sequences to identify the maternal and paternal lineage, respectively.ConclusionThese molecular diagnostic methods could also be used to manage breeding plans of hybrids, monitor and minimize the negative impacts of hybridization programs in aquaculture. Furthermore, our study could also provide a reference for establishing detection technique for hybrids in other groups of fishes.

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“…Consumer safety is put at risk by allergens, interactions with medication or supplements (in the case of mislabelled herb or plant products), and toxicity (Clark 2015). A lack of confidence in industry can result in behaviour change and damage to the revenue and reputation of suppliers, with trickle-down effects to retailers, restaurants, and other food outlets (Barnett et al 2016).…”

Section: Preventing the Mislabelling Of Food Productsmentioning

confidence: 99%

“…Procedures developed in ancient DNA laboratories mean that even very small amounts of degraded DNA can be used in combination with HTS techniques, whereas reasonably high-quality DNA is needed for Sanger sequencing. Identification by third parties can be auditable, and a subjective element of identification is removed due to its reliance on a reference library (Clark 2015), once these libraries are built and validated to obtain accurate identifications. Open access tools will increase reproducibility of barcode data further-bioinformatics scripts can be uploaded to github, and data can be stored in online repositories such as EBI, NCBI, DataDryad, or Figshare, although long-term accessible storage space for large datasets is under pressure.…”

Section: Issues and Implementationmentioning

confidence: 99%

“…Agri-food supply chains are becoming elongated as they are globalized, which exposes consumers to the risks of greater potential for contamination, misidentification, and even food fraud (Lee et al 2012). Although the application of molecular techniques has been reviewed for certain aspects of the food industry and the ecosystems on which it depends (e.g., food fraud (Clark 2015), host-parasitoid interactions (Hrcek and Godfray 2015)), a review of the applications of DNA barcoding throughout the entire food chain from farm to fork, with a particular emphasis on the use of the new technology of metabarcoding, is lacking. In this article, we will review the potential applications of DNA barcoding in agriculture, from monitoring communities under threat in agro-ecosystems, ensuring food security against attack from pests and parasites, to providing safe and traceable food to the kitchen table (Fig.…”

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confidence: 99%

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Barcoding the food chain: from Sanger to high-throughput sequencing

Littlefair

Clare

2016

Genome

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Society faces the complex challenge of supporting biodiversity and ecosystem functioning, while ensuring food security by providing safe traceable food through an ever-more-complex global food chain. The increase in human mobility brings the added threat of pests, parasites, and invaders that further complicate our agro-industrial efforts. DNA barcoding technologies allow researchers to identify both individual species, and, when combined with universal primers and high-throughput sequencing techniques, the diversity within mixed samples (metabarcoding). These tools are already being employed to detect market substitutions, trace pests through the forensic evaluation of trace "environmental DNA", and to track parasitic infections in livestock. The potential of DNA barcoding to contribute to increased security of the food chain is clear, but challenges remain in regulation and the need for validation of experimental analysis. Here, we present an overview of the current uses and challenges of applied DNA barcoding in agriculture, from agro-ecosystems within farmland to the kitchen table.

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The current status of DNA barcoding technology for species identification in fish value chains

Cited by 51 publications

References 25 publications

Increasing the functionalities and accuracy of fisheries electronic monitoring systems

Increasing the functionalities and accuracy of fisheries electronic monitoring systems

Molecular diagnostic markers of Tachysurus fulvidraco and Leiocassis longirostris and their hybrids

Barcoding the food chain: from Sanger to high-throughput sequencing

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