DNA‐Based Methods for the Identification of Commercial Fish and Seafood Species

Rasmussen, Rosalee S.; Morrissey, Michael T.

doi:10.1111/j.1541-4337.2008.00046.x

Cited by 295 publications

(209 citation statements)

References 140 publications

(306 reference statements)

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“…However, these methods are not suitable for routine analysis because proteins lose their biological characteristics or become degraded in processed foods (Rasmussen et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

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A Comparison of Eight Methods for DNA Etraction from Processed Seafood Products

Jiang

et al. 2016

FSTR

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DNA extraction is critical for seafood authentication based on DNA techniques. In this study, eight extraction methods, including commercial kit, from three types of seafood (quick frozen, roasted, and canned products) were compared in terms of DNA yield and purity. The quantity and quality of extracted DNA were evaluated using the ratio A260/A280. Quality was also evaluated by two pairs of primers that could amplify two different sizes of DNA fragments respectively. For quick frozen samples, the results showed that the top three methods for DNA yield were the SDS method, TIANGEN GMO food DNA Extraction Kit, and TIANamp Marine Animals DNA Kit.For roasted samples, the top two methods for DNA yield were the SDS and improved CTAB methods. For canned samples, the top two methods for DNA yield were the SDS and phenol/chloroform/isoamylic alcohol methods. The differences between these aforementioned methods and the remaining methods were highly significant (P < 0.01).The DNA extracted from roasted and canned seafood could not amplify the 650 bp but amplified the 358 bp target fragment. This study determined the proper DNA extraction method for three types of seafood to facilitate seafood authentication.

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“…However, these methods are not suitable for routine analysis because proteins lose their biological characteristics or become degraded in processed foods (Rasmussen et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…The authentication of fish and seafood species has become an important issue in the seafood industry (Rasmussen et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

A Comparison of Eight Methods for DNA Etraction from Processed Seafood Products

Jiang

et al. 2016

FSTR

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“…Food authenticity and food safety testing is carried out on an international scale by a number of government and private testing laboratories [15,16,17]. Of recent concern is fisheries food fraud of which there are 7 distinct forms: species substitution; fishery substitution; illegal, unreported and unregulated (IUU) substitution; species adulteration; chain of custody abuse; catch method fraud; and undeclared product extension [14].…”

Section: Introductionmentioning

confidence: 99%

“…Of recent concern is fisheries food fraud of which there are 7 distinct forms: species substitution; fishery substitution; illegal, unreported and unregulated (IUU) substitution; species adulteration; chain of custody abuse; catch method fraud; and undeclared product extension [14]. An increase in global seafood consumption has led to a rise in product mislabelling, and its effects are far reaching [17]. In 2009, a fifth of the annual global seafood catch came from IUU fishing [18].…”

Section: Introductionmentioning

confidence: 99%

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Species detection using HyBeacon ® probe technology: Working towards rapid onsite testing in non-human forensic and food authentication applications

Dawnay

Hughes

Court

et al. 2016

Forensic Science International: Genetics

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26Identifying individual species or determining species' composition in an unknown sample is important 27 for a variety of forensic applications. Food authentication, monitoring illegal trade in endangered 28 species, forensic entomology, sexual assault case work and counter terrorism are just some of the fields 29 that can require the detection of the biological species present. Traditional laboratory based approaches 30 employ a wide variety of tools and technologies and exploit a number of different species specific traits 31 including morphology, molecular differences and immuno-chemical analyses. A large number of these 32 approaches require laboratory based apparatus and results can take a number of days to be returned to 33 investigating authorities. Having a presumptive test for rapid identification could lead to savings in terms 34 of cost and time and allow sample prioritisation if confirmatory testing in a laboratory is required later. 35This model study describes the development of an assay using a single HyBeacon ® probe and melt curve 36 analyses allowing rapid screening and authentication of food products labelled as Atlantic cod (Gadus 37 morhua). Exploiting melt curve detection of species specific SNP sites on the COI gene the test allows 38 detection of a target species (Atlantic cod) and closely related species which may be used as substitutes. 39The assay has been designed for use with the Field Portable ParaDNA system, a molecular detection 40 platform for non-expert users. The entire process from sampling to result takes approximately 75 41 minutes. Validation studies were performed on both single source genomic DNA, mixed genomic DNA 42 and commercial samples. Data suggests the assay has a lower limit of detection of 31 pg DNA. The 43 specificity of the assay to Atlantic cod was measured by testing highly processed food samples including 44 frozen, defrosted and cooked fish fillets as well as fish fingers, battered fish fillet and fish pie Ninety-45 six (92.7 %) of all Atlantic cod food products tested provided a correct single species result with the 46 remaining samples erroneously identified as containing non-target species. The data shows that the assay 47 was quick to design and characterise and is also capable of yielding results that would be beneficial in 48 a variety of fields, not least the authentication of food. 49 50 3

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