Species Identification of Seafood

Applewhite, LeeAnn; Rasmussen, Rosalee S.; Morrissey, Michael T.

doi:10.1002/9781118229491.ch16

Cited by 3 publications

(2 citation statements)

References 132 publications

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“…Various studies were carried out to investigate and implement the use of these rRNA genes as molecular markers for species identification. Variants belonging to 12S and 16S molecular markers have been used to identify a wide variety of flatfish, eel, cardinalfish, cephalopods, mackerel, hairtail species, crab and several others [ 39 , 128 , 130 , 131 ]. Worldwide, 12S and 16S rDNAs have become valuable barcoding tools to assess seafood fraud verification cases.…”

Section: Dna Barcode Regionsmentioning

confidence: 99%

Molecular Barcoding: A Tool to Guarantee Correct Seafood Labelling and Quality and Preserve the Conservation of Endangered Species

Filonzi

Ardenghi

Rontani

et al. 2023

Foods

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The recent increase in international fish trade leads to the need for improving the traceability of fishery products. In relation to this, consistent monitoring of the production chain focusing on technological developments, handling, processing and distribution via global networks is necessary. Molecular barcoding has therefore been suggested as the gold standard in seafood species traceability and labelling. This review describes the DNA barcoding methodology for preventing food fraud and adulteration in fish. In particular, attention has been focused on the application of molecular techniques to determine the identity and authenticity of fish products, to discriminate the presence of different species in processed seafood and to characterize raw materials undergoing food industry processes. In this regard, we herein present a large number of studies performed in different countries, showing the most reliable DNA barcodes for species identification based on both mitochondrial (COI, cytb, 16S rDNA and 12S rDNA) and nuclear genes. Results are discussed considering the advantages and disadvantages of the different techniques in relation to different scientific issues. Special regard has been dedicated to a dual approach referring to both the consumer’s health and the conservation of threatened species, with a special focus on the feasibility of the different genetic and genomic approaches in relation to both scientific objectives and permissible costs to obtain reliable traceability.

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Section: Dna Barcode Regionsmentioning

confidence: 99%

Molecular Barcoding: A Tool to Guarantee Correct Seafood Labelling and Quality and Preserve the Conservation of Endangered Species

Filonzi

Ardenghi

Rontani

et al. 2023

Foods

View full text Add to dashboard Cite

show abstract

“…While this method has proved somewhat dependable when testing unprocessed seafood under cold storage, it has been described as unreliable when analyzing cooked tissues or fillets packaged with acidic sauces or marinades to increase value (Applewhite, Rasmussen, & Morrissey, 2012;Rasmussen & Morrissey, 2008). Furthermore, IEF procedures are tedious, often require several days, and interpretation of results can be subjective (Applewhite et al, 2012).…”

Section: Introductionmentioning

confidence: 99%