DNA-based nanocomposite as electrochemical chiral sensing platform for the enantioselective interaction with quinine and quinidine

Zhang, Qing; Huang, Yihan; Guo, Lingyu; Cui, Chen; Guo, Dongmei; Ya, Chen; Fu, Yingzi

doi:10.1039/c3nj01559a

Cited by 26 publications

(20 citation statements)

References 38 publications

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“…11 Importantly, acute and subchronic adult neurotoxicity also resulted from long-term exposure to RES. 13 It is quite often the main molecular target of drugs and harmful substances. 13 It is quite often the main molecular target of drugs and harmful substances.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the interaction between resmethrin and calf thymus DNA in vitro

et al. 2015

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Resmethrin (RES) is a synthetic pyrethroid insecticide widely used to control pests in agriculture, but it may cause potential hazards to human health. The characteristics of the binding of RES with calf thymus DNA (ctDNA) were investigated in a physiological buffer (pH 7.4) by multiple spectroscopic methods combined with ctDNA melting and viscosity measurements, multivariate curve resolution-alternating least-squares (MCR-ALS) chemometrics and the molecular docking technique. The concentration profiles and the pure spectra for the reactive species (RES, ctDNA and RES-ctDNA complex), obtained through decomposing the augmented UV-vis spectral data matrix by the MCR-ALS approach, indicated that RES could bind to ctDNA and the reaction process could be quantitatively monitored. The RES molecules bound to ctDNA by groove binding, as evidenced by the negligible changes in the ctDNA melting temperature, viscosity and iodide quenching effect and the increase in the single-stranded DNA quenching effect. Fluorescence titration data indicated that the complexation of RES with ctDNA was mainly driven by hydrogen bonds and van der Waals forces, and had strong affinity. The changes in the Fourier transform infrared spectra of ctDNA suggested that RES molecules preferentially bound to the G-C region of ctDNA, which was consistent with the prediction of the molecular docking. The circular dichroism spectral analysis indicated that RES induced a decrease in the right-handed helicity of ctDNA.The DNA cleavage assay showed that RES did not cleave the pUC18 plasmid DNA. This study offers a comprehensive picture of RES-ctDNA interaction, which may provide insights into the toxicological effect of the insecticide.

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Section: Introductionmentioning

confidence: 99%

Characterization of the interaction between resmethrin and calf thymus DNA in vitro

et al. 2015

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“…59 As compared to Tween-20 and SDBS, addition of CTAB to the solution containing Qn exhibited appreciable enhancement in reduction peak current, a greater shift in cathodic peak potential and a lower detection limit. 61 The electrode was simple to prepare, stable as well as reproducible. The method displayed excellent accuracy, precision and reproducibility.…”

Section: Quininementioning

confidence: 99%

“…100,106 Enzymes are also used as labels attached to antigens, antibodies and oligonucleotides with a specific sequence providing affinity-based sensors. 46,61,80,118 polypyrrole modified electrodes were formed on PGE and successfully used to determine the drug. 134 The drug was quantified by competitive binding between DC and a HRP-labeled specific tracer for binding sites of the captured antibodies immobilized on the surface of protein-functionalized magnetic beads.…”

Section: Recognition Receptors Based Electrochemical Analysis Of Antimentioning

confidence: 99%

Research progress in electroanalytical techniques for determination of antimalarial drugs in pharmaceutical and biological samples

et al. 2016

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“…However, these methods require expensive chiral columns, relatively long analysis times and complicated sample pre-treatments. Recently, electrochemical approaches based on various chemically modified electrodes have been widely used in chiral recognition of propranolol [4], tyrosine [10], quinine and quinidine [11] due to their speed, simplicity, low cost, high sensitivity and enantioselective electrochemical signals.…”

Section: Introductionmentioning

confidence: 99%

Novel N-Doped Carbon Dots/β-Cyclodextrin Nanocomposites for Enantioselective Recognition of Tryptophan Enantiomers

Xiao¹,

Lu²,

Huang³

et al. 2016

Sensors

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Based on N-doped carbon dots/β-cyclodextrin nanocomposites modified glassy carbon electrodes (N-CDs/β-CD/GCE), an effective electrochemical sensor for enantioselective recognition of tryptophan (Trp) enantiomers was developed by differential pulse voltammograms (DPVs). Fluorescent N-CDs were synthesized through a hydrothermal method and characterized by spectroscopic approaches. The N-CDs/β-CD nanocomposites were efficiently electrodeposited on the surface of GCE through C–N bond formation between N-CDs and electrode. The obtained N-CDs/β-CD/GCE was characterized by multispectroscopic and electrochemical methods. Such N-CDs/β-CD/GCE generated a significantly lower Ip and more negative Ep in the presence of l-Trp in DPVs, which was used for the enantioselective recognition of Trp enantiomers. The N-CDs/β-CD nanocomposites showed different binding constants for tryptophan enantiomers, and they further selectively bonded with l-Trp to form inclusion complexes. This N-CDs/β-CD/GCE combined advantages of N-CDs with strong C–N binding ability and β-CD with specific recognition of Trp enantiomers to fabricate a novel sensing platform for enantioselective recognition of Trp enantiomers. This strategy provided the possibility of using a nanostructured sensor to discriminate the chiral molecules in bio-electroanalytical applications.

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DNA-based nanocomposite as electrochemical chiral sensing platform for the enantioselective interaction with quinine and quinidine

Abstract: A DNA-based nanocomposite was prepared to develop a simple strategy for electrochemical chiral analysis.

Cited by 26 publications

References 38 publications

Characterization of the interaction between resmethrin and calf thymus DNA in vitro

Characterization of the interaction between resmethrin and calf thymus DNA in vitro

Research progress in electroanalytical techniques for determination of antimalarial drugs in pharmaceutical and biological samples

Novel N-Doped Carbon Dots/β-Cyclodextrin Nanocomposites for Enantioselective Recognition of Tryptophan Enantiomers

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