DNA binding and cleavage studies of copper(II) complex containing N2O2 Schiff base ligand

Thi

Bioinorganic Chemistry and Applications

2021

Unsymmetrical tetradentate Schiff base Fe(III) and Cu(II) complexes were prepared by the coordination of some unsymmetrical tetradentate Schiff base ligands with CuCl2·2H2O or FeCl3·6H2O. The obtained complexes were characterized by ESI-MS, IR, and UV-Vis. The spectroscopic data with typical signals are in agreement with the suggested molecular formulae of the complexes. Their cyclic voltammetric studies in acetonitrile solutions showed that the Cu(II)/Cu(I) and Fe(III)/Fe(II) reduction processes are at (−)1.882–(−) 1.782 V and at (−) 1.317–(−) 1.164 V, respectively. The in vitro cytotoxicity of obtained complexes was screened for KB and Hep-G2 human cancer cell lines. The results showed that almost unsymmetrical tetradentate Schiff base complexes have good cytotoxicity. The synthetic complexes bearing the unsymmetrical tetradentate Schiff base ligands with different substituted groups in the salicyl ring indicate different cytotoxicity. The obtained Fe(III) complexes are more cytotoxic than Cu(II) complexes and relative unsymmetric Schiff base ligands.

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and In Vitro Cytotoxicity of Unsymmetrical Tetradentate Schiff Base Cu(II) and Fe(III) Complexes

Thi

Bioinorganic Chemistry and Applications

2021

“…In addition, copper ions may interact directly with the DNA or affect the electrophoretic mobility of the molecule [ 12 ]. Copper ligands are known to disturb DNA helix resulting in DNA conformational changes and DNA breakages [ 13 ] as indicated by docking studies [ 14 ]. They are also considered as putative drugs against cancer [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants

Bednarek

Orłowska

2020

Plants

Plant anther culture allows for the regeneration of uniform and homozygous double haploids. However, off-type regenerants may appear as a result of so-called tissue culture-induced variation (TCIV). In addition, the presence of Cu2+ and Ag+ ions in the culture medium might influence the number of green plants. The regenerants were obtained via anther cultures of barley under varying Cu2+ and Ag+ ion concentrations in the induction medium during distinct time conditions. DArTseqMet markers were evaluated based on regenerants and donor plants and delivering data on DNA demethylation (DM) and de novo methylation (DNM) and changes in methylation (Delta). The number of green regenerated plants per 100 anthers (GPs) was evaluated. The Cu2+ and Ag+ ion concentrations moderated relationships between Delta and the number of green plants conditional on time of tissue cultures. Depending on the ions, moderated moderation is valid within the different time of anther culture. When the highest concentration of copper is analyzed, plant regeneration is possible under short ‘Time’ (21 days) of anther culture wherein Delta is negative or under elongated Time when Delta is positive. Under 21 days of culture, the highest concentration of silver ions and when Delta is negative, some regenerants could be evaluated. However, under high Ag+ concentration when Time of culture is long and Delta positive, the highest number of green plants could be obtained.

“…K b values were found out to be 1.351 × 10 4 and 1.361 × 10 4 M −1 , respectively, for complexes 1 and 2 . These values are comparable to those observed for other copper(II) complexes calculated through absorption and florescence measurements techniques …”

Section: Resultsmentioning

confidence: 76%

“…The decrease in the current of the complexes with DNA addition was used to calculate the binding constant of the complexes with DNA using the plot of current and concentration of DNA shown in Figures b and b. The K b values determined in this manner were 1.820 × 10 4 M −1 and 2.426 × 10 4 M −1 , respectively, for complexes 1 and 2 , which are comparable to that of other copper complexes …”

Section: Resultsmentioning

confidence: 99%

Mononuclear vs. binuclear carboxylates of copper(II) with 2,2′‐bipyridine: Synthesis, characterization, structural description, and properties

Iqbal

Ali

Sohail³

et al. 2019

J Chinese Chemical Soc

Two new copper(II) carboxylate complexes with 2,2′‐bipyridine and para‐nitrophenyl acetate (complex 1) and phenyl acetate (complex 2) have been synthesized; isolated in quantitative yield; and characterized using fourier‐transform infrared spectroscopy (FT‐IR), electron paramagnetic resonance, absorption spectroscopy, electrochemistry, and powder and single crystal X‐ray diffraction (XRD) techniques. Being mononuclear, the geometry around copper in complex 1 is a Jahn–Teller distorted octahedral, while complex 2 is binuclear with slightly distorted square pyramidal geometry around both copper ions. Powder XRD indicated several peaks in spectra of both complexes, which coincided with their theoretical spectra. FT‐IR results of the carboxylate stretching frequency were in accordance with the single crystal structure data. Electron paramagnetic resonance spectra of complexes 1 and 2 yielded g┴ values of 2.06161 and 2.24623 and 1.94959, respectively, indicating a localized electron in b1 (d x2–y2‐orbital). Ultra‐violet (UV)–visible spectroscopy and electrochemistry helped in characterization, as well as in deoxyribonucleic acid (DNA)‐binding ability of the complexes, yielding DNA‐binding constant values = 1.351 × 104 and 1.361 × 104 and 1.820 × 104 and 2.426 × 104 M−1, respectively, for complexes 1 and 2. The complexes demonstrate good biological potential.