DNA-binding, antioxidant, H₂O₂ sensing and photocatalytic properties of biogenic silver nanoparticles using Ageratum conyzoides L. leaf extract

Abstract

Green nanotechnology is gaining widespread interest owing to the elimination of harmful reagents and offers a cost-effective synthesis of expected products. In the present study, silver nanoparticles (AgNPs) were synthesized from Ageratum conyzoides leaf extract (ACLE). UV-visible spectrophotometry showing a characteristic SPR peak at 443 nm verified the phytosynthesis of AC-AgNPs. The FTIR spectrum was examined to identify the efficient functional molecules responsible for the reduction of Ag + to metallic silver (Ag 0 ). SEM, TEM and XRD illustrated the formation of crystalline and spherical NPs with a size range of 14-48 nm. EDX data showed the presence of elemental silver with an energy peak at 3 eV. CT-DNA interaction with AC-AgNPs was investigated and the UV absorption spectra revealed a bathochromic effect indicating groove binding. AC-AgNPs showed a strong antioxidant property in a concentrationdependent manner when analyzed by DPPH and ABTS radical scavenging assay. AC-AgNPs were investigated as a SPR-based H 2 O 2 sensor, which can provide promising opportunities in medical and environmental fields to detect reactive oxygen species such as H 2 O 2 . The catalytic effectiveness of phytosynthesized NPs was also examined within 2 h of exposure for methylene blue degradation under sunlight. There is thus a reasonable potential application of green synthesized AC-AgNPs for the degradation of hazardous synthetic dyes.

Cytotoxic, Antimitotic, DNA Binding, Photocatalytic, H2O2 Sensing, and Antioxidant Properties of Biofabricated Silver Nanoparticles Using Leaf Extract of Bryophyllum pinnatum (Lam.) Oken

“…Studies of DNA interaction were performed by the method described by Chandraker et al ( 2019b ), Different concentrations of CT-DNA (20–260 μl) in Tris-HCl buffer (pH 7.2) were treated with BP-AgNPs (10 μM) in 1 % aqueous DMSO. UV-visible spectrophotometer further examined the specific concentration of the combined solution of CT-DNA and BP-AgNPs.…”

“…The physical methods utilize costly equipments and produce unstable larger nanoparticles, while hydrazine and sodium borohydride like hazardous materials are used as reducing agents in the chemical approaches (Mohanta et al, 2017 ; Chandraker et al, 2019a ). Likewise, cautious isolation of bacteria, fungi, and the management of their non-contaminated culture or their byproducts are tedious issues with the microbial synthesis of NPs (Chandraker et al, 2019b ).…”

Green synthesis of copper nanoparticles using leaf extract of Ageratum houstonianum Mill. and study of their photocatalytic and antibacterial activities

“…A control set of experiment was also designed for the comparative study to determine the changes in color of the dye solutions in absence of AH-CuNPs. The absorption of the dye solutions was recorded at constant time intervals (every 20 min) [45]. The Langmuir-Hinshelwood kinetic model was used to determine the rate of photocatalytic reactions [48,49].…”

Therapeutic potential of biogenic and optimized silver nanoparticles using Rubia cordifolia L. leaf extract

“…The antioxidant properties of RC@AgNPs were determined using the DPPH and ABTS free radical scavenging tests described by Chandraker et al 25 . Using UV–visible spectroscopy, the antioxidant potential of RC@AgNPs was tested in the presence of DPPH and ABTS free radicals, and ascorbic acid was used as a standard reference.…”