DNA‐binding proteins of the Whirly family in <i>Arabidopsis thaliana</i> are targeted to the organelles

Krause, Kirsten; Kilbienski, Isabell; Mulisch, Maria; Rödiger, Anja; Schäfer, Anke; Krupinska, Karin

doi:10.1016/j.febslet.2005.05.059

Cited by 110 publications

(135 citation statements)

References 22 publications

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“…Furthermore, pTAC1 and pTAC11 exhibit striking similarities to p24 proteins, which are members of the Whirly proteins of nuclear transcription factors (Desveaux et al, 2002(Desveaux et al, , 2004. All Whirly proteins have target sequences for organelles (Krause et al, 2005); thus, they might be dually targeted to the nucleus and the plastid. The exact functions of the pTAC proteins remain to be determined; however, the striking similarities of the observed phenotypes of the knockout lines for three of these proteins with those of Drpo mutants and other mutants impaired in PEP function suggests that they are also involved in plastid gene expression.…”

Section: Discussionmentioning

confidence: 99%

pTAC2, -6, and -12 Are Components of the Transcriptionally Active Plastid Chromosome That Are Required for Plastid Gene Expression

et al. 2005

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Transcription in plastids is mediated by a plastid-encoded multimeric (PEP) and a nuclear-encoded single-subunit RNA polymerase (NEP) and a still unknown number of nuclear-encoded factors. By combining gel filtration and affinity chromatography purification steps, we isolated transcriptionally active chromosomes from Arabidopsis thaliana and mustard (Sinapis alba) chloroplasts and identified 35 components by electrospray ionization ion trap tandem mass spectrometry. Eighteen components, called plastid transcriptionally active chromosome proteins (pTACs), have not yet been described. T-DNA insertions in three corresponding genes, ptac2, -6, and -12, are lethal without exogenous carbon sources. Expression patterns of the plastid-encoded genes in the corresponding knockout lines resemble those of Drpo mutants. For instance, expression of plastid genes with PEP promoters is downregulated, while expression of genes with NEP promoters is either not affected or upregulated in the mutants. All three components might also be involved in posttranscriptional processes, such as RNA processing and/or mRNA stability. Thus, pTAC2, -6, and -12 are clearly involved in plastid gene expression.

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Section: Discussionmentioning

confidence: 99%

pTAC2, -6, and -12 Are Components of the Transcriptionally Active Plastid Chromosome That Are Required for Plastid Gene Expression

et al. 2005

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“…Examples are PEND, CND41, PD1, PD3 (Kodama 2007) as well as Whirly1 (Krause et al 2005;Grabowski et al 2008) and the newly identified ptNAP SWIB-4 (Melonek et al 2012). It has been suggested that these proteins have changed their subcellular localization during evolution and became plastid or dually targeted proteins.…”

Section: Bifunctionality and Dual Targeting Of Ptnapsmentioning

confidence: 99%

New insights into plastid nucleoid structure and functionality

Krupinska¹,

Melonek²,

Krause

2012

Planta

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Investigations over many decades have revealed that nucleoids of higher plant plastids are highly dynamic with regard to their number, their structural organization and protein composition. Membrane attachment and environmental cues seem to determine the activity and functionality of the nucleoids and point to a highly regulated structure-function relationship. The heterogeneous composition and the many functions that are seemingly associated with the plastid nucleoids could be related to the high number of chromosomes per plastid. Recent proteomic studies have brought novel nucleoidassociated proteins into the spotlight and indicated that plastid nucleoids are an evolutionary hybrid possessing prokaryotic nucleoid features and eukaryotic (nuclear) chromatin components, several of which are dually targeted to the nucleus and chloroplasts. Future studies need to unravel if and how plastid-nucleus communication depends on nucleoid structure and plastid gene expression.

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“…van Wijk, unpublished data). Plastid pTAC11 is a member of the Whirly family of multifunctional RNA-and DNA-binding proteins located in mitochondria and plastids (Krause et al, 2005). Members of the Whirly family are involved in organelle genome stability and quality control and, perhaps, RNA metabolism (Prikryl et al, 2008;Maréchal et al, 2009;Cappadocia et al, 2012).…”

Section: Effects On Plastid Gene Expression and Protein Homeostasismentioning

confidence: 99%

Modified Clp Protease Complex in the ClpP3 Null Mutant and Consequences for Chloroplast Development and Function in Arabidopsis

Kim

Olinares

et al. 2013

Plant Physiology

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The plastid ClpPRT protease consists of two heptameric rings of ClpP1/ClpR1/ClpR2/ClpR3/ClpR4 (the R-ring) and ClpP3/ ClpP4/ClpP5/ClpP6 (the P-ring) and peripherally associated ClpT1/ClpT2 subunits. Here, we address the contributions of ClpP3 and ClpP4 to ClpPRT core organization and function in Arabidopsis (Arabidopsis thaliana). ClpP4 is strictly required for embryogenesis, similar to ClpP5. In contrast, loss of ClpP3 (clpp3-1) leads to arrest at the hypocotyl stage; this developmental arrest can be removed by supplementation with sucrose or glucose. Heterotrophically grown clpp3-1 can be transferred to soil and generate viable seed, which is surprising, since we previously showed that CLPR2 and CLPR4 null alleles are always sterile and die on soil. Based on native gels and mass spectrometry-based quantification, we show that despite the loss of ClpP3, modified ClpPR core(s) could be formed, albeit at strongly reduced levels. A large portion of ClpPR subunits accumulated in heptameric rings, with overaccumulation of ClpP1/ClpP5/ClpP6 and ClpR3. Remarkably, the association of ClpT1 to the modified Clp core was unchanged. Large-scale quantitative proteomics assays of clpp3-1 showed a 50% loss of photosynthetic capacity and the up-regulation of plastoglobules and all chloroplast stromal chaperone systems. Specific chloroplast proteases were significantly up-regulated, whereas the major thylakoid protease (FtsH1/FtsH2/FtsH5/FtsH8) was clearly unchanged, indicating a controlled protease network response. clpp3-1 showed a systematic decrease of chloroplast-encoded proteins that are part of the photosynthetic apparatus but not of chloroplast-encoded proteins with other functions. Candidate substrates and an explanation for the differential phenotypes between the CLPP3, CLPP4, and CLPP5 null mutants are discussed.

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DNA‐binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles

Cited by 110 publications

References 22 publications

pTAC2, -6, and -12 Are Components of the Transcriptionally Active Plastid Chromosome That Are Required for Plastid Gene Expression

pTAC2, -6, and -12 Are Components of the Transcriptionally Active Plastid Chromosome That Are Required for Plastid Gene Expression

New insights into plastid nucleoid structure and functionality

Modified Clp Protease Complex in the ClpP3 Null Mutant and Consequences for Chloroplast Development and Function in Arabidopsis

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