DNA-bound lipids of normal and tumor cells: retrospective and outlooks for functional genomics

Struchkov, V. A.; Strazhevskaya, N. B.; Жданов, Р. И.

doi:10.1016/s1567-5394(02)00129-9

Cited by 24 publications

(13 citation statements)

References 25 publications

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“…The assay of the assimilatory pigments extracts (in 80% acetone) was performed following the Lichtenthaler and Welburn's method, 16 while the assay of nucleic acid level (in perchloric acid 6% extracts) was carried out accordingly to modified Spirin's method. 17,18 Spectrophotometric measurements were performed at the wavelengths of: 663 nm, 646 nm and 470 nm (versus acetone 80%) for the assay of chlorophylls (Chla, Chlb) and carotenoids pigments (Car) from green tissues and, at 260 nm and 280 nm (versus perchloric acid 6%) in the case of nucleic acids. For the calculation of photosynthetic pigments, the formulae from Lichtenthaler and Welburn 16 were applied while in the case of nucleic acids calibration curves (based on the spectral readings to the mentioned wavelengths) were used.…”

Section: Analysis Methodsmentioning

confidence: 99%

Iron oxide nanoparticles coated with β-cyclodextrin polluted of Zea mays plantlets

Racuciu

2012

Nanotechnol Dev

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The present experimental investigation is focused on the study of assimilatory pigments and nucleic acid levels in young plants intended for agricultural use (Zea mays) in presence of water based magnetic fluid added in culture medium. The magnetic fluid was constituted by coating the nanosized magnetic nanoparticles (with 10.55 nm average value of the physical diameter) with β-cyclodextrin (C 42 H 70 O 35 ) and further dispersion in water. After germination, various volume fractions (between 10 mL/L and 500 mL/L) of the magnetic fluid was added daily in the culture medium of Zea mays plants still at their early ontogenetic stages. Toxicity symptoms leaded to brown spots covering the leaf surface for the highest magnetic fluid volume fractions used, a putative oxidative stress generated by iron excess treatment. Relatively small volume fraction of magnetic fluid solutions induced the increase of chlorophyll a level (up to 38%), the main photosynthesis pigment, as well that the nucleic acid level (up to 57%) in Zea mays plantlets. All volume fractions of magnetic fluid solutions analyzed may have severe disruptive effects such as the ratio chlorophyll a/chlorophyll b (about 50% decreasing).

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Section: Analysis Methodsmentioning

confidence: 99%

Iron oxide nanoparticles coated with β-cyclodextrin polluted of Zea mays plantlets

Racuciu

2012

Nanotechnol Dev

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“…The genomic DNA was isolated from cells by the phenol method, yielding high molecular mass DNA (method 1) (Balint, 1987;Zhdanov et al, 2001;Struchkov et al, 2002) or a detergent extraction-based procedure (method 2) (Cunha et al, 2001) (Fig. 1).…”

Section: Isolation Of Genomic Dnamentioning

confidence: 99%

“…Although DNA-bound lipids isolated from prokaryotic and eukaryotic cells were reported to represent an integral part of nucleoid and chromatin (Balint, 1987;Struchkov & Strazhevskaya, 1993), this fact has not yet been proven and commonly accepted. The origin and functions of specific DNA-bound lipids remain unclear (Struchkov et al, 2002). However, some reports indicate that cellular lipids, fatty acids, in particular, play an important metabolic role in cells as signaling molecules involved in the regulation of gene expression (Duplus et al, 2000;Albi & Viola Magni, 2004;Jump, 2004;Ledeen & Wu, 2004).…”

Section: Introductionmentioning

confidence: 99%

Nature of DNA-bound fatty acids inPseudomonas aurantiaca

Жданов

Shmyrina

Mulyukin

et al. 2006

FEMS Microbiology Letters

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The existence of Pseudomonas aurantiaca DNA-bound fatty acids and lipids is presented in this work. The isolation of DNA was carried out by two different procedures, namely, phenol and detergent-based phenol isolation in order to prove the presence of DNA-bound lipids. The lipid content of DNA is expressed in terms of fatty acid profile. A high level of 16:0, 18:0 and 18:1 is characteristic for tightly bound DNA lipids. On the other hand, the fatty acids such as 14:1, iso14:0 and iso16:0 are found in trace amounts only in DNA lipid fraction, but these fatty acids are not found in the whole-cell lipids. Absolutely no 3-hydroxy fatty acids were found in DNA lipids. However, both C16 and C18 species represent the main fatty acids of whole-cell and DNA-bound lipids. The presence of DNA-bound lipids even under tough treatment of DNA allows to conclude that these lipids represent a special pool among cellular lipids.

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“…This must not mean, however, that the interactions of anionic phospholipids and Z-DNA with nuclear matrix-associated proteins, like cIF proteins (this study) and annexins (Krishna et al, 1990), are functionally mutually exclusive at the cellular level. On the contrary, considering the substantial pool and metabolism of nonmembrane lipids, especially members of the phosphatidylinositol signal transduction cascade, in the nucleus and the influence of lipids on nuclear and chromatin structure as well as on DNA and RNA metabolism (Maraldi et al, 1999;Martelli et al, 2002;Struchkov et al, 2002), it is conceivable that anionic phospholipids control, among other things, the interaction of Z-DNA with Z-DNA-binding proteins as an essential component of the DNA's potential involvement in gene regulation. Such a possibility would rest on the assumption that, occurring in the regulatory regions of many eukaryotic genes (Schroth et al, 1992) and, at the same time, flanking MAR motifs (Boulikas, 1993(Boulikas, , 1995, Z-DNA tracts come under the influence of anionic phospholipids that are known to mediate the hydrophobic attachment of the MAR elements of chromatin loops to the protein constituents of the nuclear matrix (Maraldi et al, 1984;Cocco et al, 1987).…”

Section: Mode Of Interaction Of Cif Proteins With Z-dnamentioning

confidence: 99%

InteractionIn Vitroof Type III Intermediate Filament Proteins with Z-DNA and B-Z-DNA Junctions

Tolstonog

Traub

2003

DNA and Cell Biology

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The selection of DNA fragments containing simple d(GT)(n) and composite d(GT)(m). d(GA)(n) microsatellites during affinity binding of mouse genomic DNA to type III cytoplasmic intermediate filaments (cIFs) in vitro, and the detection of such repeats, often as parts of nuclear matrix attachment region (MAR)-like DNA, in SDS-stable DNA-vimentin crosslinkage products isolated from intact fibroblasts, prompted a detailed study of the interaction of type III cIF proteins with left-handed Z-DNA formed from d(GT)(17) and d(CG)(17) repeats under the topological tension of negatively supercoiled plasmids. Although d(GT)(n) tracts possess a distinctly lower Z-DNA-forming potential than d(CG)(n) tracts, the filament proteins produced a stronger electrophoretic mobility shift with a plasmid carrying a d(GT)(17) insert than with plasmids containing different d(CG)(n) inserts, consistent with the facts that the B-Z transition of d(GT)(n) repeats requires a higher negative superhelical density than that of d(CG)(n) repeats and the affinity of cIF proteins for plasmid DNA increases with its superhelical tension. That both types of dinucleotide repeat had indeed undergone B-Z transition was confirmed by S1 nuclease and chemical footprinting analysis of the plasmids, which also demonstrated efficient protection by cIF proteins from nucleolytic and chemical attack of the Z-DNA helices as such, as well as of the flanking B-Z junctions. The analysis also revealed sensibilization of nucleotides in the center of one of the two strands of a perfect d(CG)(17) insert toward S1 nuclease, indicating cIF protein-induced bending of the repeat. In all these assays, vimentin and glial fibrillary acidic protein (GFAP) showed comparable activities, versus desmin, which was almost inactive. In addition, vimentin and GFAP exhibited much higher affinities for the Z-DNA conformation of brominated, linear d(CG)(25) repeats than for the B-DNA configuration of the unmodified oligonucleotides. While double-stranded DNA was incapable of chasing the Z-DNA from its protein complexes, and Holliday junction and single-stranded (ss)DNA were distinguished by reasonable competitiveness, phosphatidylinositol (PI) and, particularly, phosphatidylinositol 4,5-diphosphate (PIP(2)) turned out to be extremely potent competitors. Because PIP(2) is an important member of the nuclear PI signal transduction cascade, it might exert a regulatory influence on the binding of cIF proteins to Z- and other DNA conformations. From this interaction of cIF proteins with Z- and bent DNA and their previously detected affinities for MAR-like, ss, triple helical, and four-way junction DNA, it may be concluded that the filament proteins play a general role in such nuclear matrix-associated processes as DNA replication, recombination, repair, and transcription.

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DNA-bound lipids of normal and tumor cells: retrospective and outlooks for functional genomics

Cited by 24 publications

References 25 publications

Iron oxide nanoparticles coated with β-cyclodextrin polluted of Zea mays plantlets

Iron oxide nanoparticles coated with β-cyclodextrin polluted of Zea mays plantlets

Nature of DNA-bound fatty acids inPseudomonas aurantiaca

InteractionIn Vitroof Type III Intermediate Filament Proteins with Z-DNA and B-Z-DNA Junctions

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